从大公交的战略和战术提出的时间交叉桥之我见

清晨,我们从家中走来,就急匆匆、身不由己地汇入川流不息的车流中.当我们要通过某一道口时,都是拼命地往前挤,每个人都努力站在离停止线最近的地方.而停止线靠近道口设置,信号基本上是红灯停、绿灯行,黄灯工作时间短暂,作用不明显.在这样拥挤的状况下,车辆零速起动,运行迟缓,经常出现东西方向车辆未走完,南北方向绿灯已亮.现在问题出来了:     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

不同纤维对沥青的吸附稳定作用研究

在沥青混合料中加入纤维加筋材料是改善混合料性能的重要研究方向之一,通过在室内对3种不同纤维对于沥青的吸持性试验研究,可以表征不同纤维对沥青吸附稳定作用的大小。     

梁式桥地震设防应注意的问题

对常规梁桥下部结构、上部结构、梁体及支座在地震作用下的结构反应进行了论述,并对采取的相应梁桥抗震设防措施进行了归纳总结,对梁桥的设计施工有一定的参考借鉴意义。     

Influence of Ca Element on Microstructure and Mechanical Properties of AZ61-1.2Y

The effects of Ca element on the microstructure and mechanical properties of AZ61-1.2Y alloy were investigated by microstructure observation, tensile tests and fracture analysis. The results show that, with the addition of Ca element, the microstructure of AZ61-1.2Y was obviously refined, high melting point Al2Ca intermetallic compound formed, and β-Mg17Al12 phase disappeared. With 1.0% Ca addition, the values of tensile strength at 25, 150 and 175 ℃ reached their maxima simultaneously, 225, 182, and 175 MP...     

对季冻区路基强度及稳定性的试验研究

以松北区低液限粉质粘土路基为研究对象,由于该区处于季冻土分布区域内,因此,在研究填筑材料的物理力学基本性质的基础上,着重进行了冻融作用对路基填土力学性质的研究初探,试验结果为进一步的深入研究打下了基础,并对该区的道路建设提供了基本的理论参考。     

震后公路重建关键技术研究全面启动

近日,“抗震重大科技专项”第一次工作会议在北京召开。据了解,“抗震重大科技专项”已于日前全面展开。该项研究暂定名为“汶川地震灾后公路重建抗震减灾关键技术研究”,将进一步发挥科技对交通基础设施灾后恢复重建的支撑作用,进一步提升交通基础设施抵御地震灾害的能力。     

朝阳市大凌河滨水景观生态环境因子特征分析

本研究对朝阳市大凌河滨水景区与市区环境因子进行了观察与分析,并以速生杨、珍珠梅为例,研究了滨水景区与市区植物光合作用的日变化,对大凌河景观的生态作用进行了科学的探讨。     

基于二次调节技术的公交汽车传动系统性能研究

阐述了双作用叶片式二次元件的结构和工作原理，分析了基于二次调节技术的公交汽车传动系统的节能机理，建立了二次调节传动系统的数学模型，提出了采用恒扭矩控制节能制动的能量回收方式，对非恒压网络下二次调节传动系统的动态性能进行了仿真研究，得出了应用二次调节技术能够回收和重新利用系统的制动动能，减小了系统的装机容量，实现了节能降耗．     

添加剂剂量对改善沥青紫外线老化作用的影响分析

利用室内紫外线加速老化系统,在确定紫外线对沥青薄膜的老化作用后,采用不同种类的紫外线吸收剂对沥青的抗紫外老化性能进行改善。室内试验结果表明,同一种添加剂在不同剂量时对沥青性能的改善程度各不相同,且某些添加剂在合适剂量时对沥青抗紫外线老化性能有着显著的改善效果。