PPAR-γ激活对血管平滑肌细胞外基质释放及结缔组织生长因子表达的影响

目的观察PPAR-γ激活对血管紧张素Ⅱ诱导的体外培养条件下大鼠血管平滑肌(VSMCs)的细胞外基质(ECM)释放及结缔组织生长因子(CTGF)表达的影响。方法原代培养大鼠VSMCs,用不同浓度PPAR-γ激动剂rosiglitzone和/或抑制剂GW9662、BADGE预处理后,加入0.1μmol/L血管紧张素Ⅱ(AngⅡ)刺激24h。比色法检测各组细胞培养液中羟脯氨酸含量,实时荧光定量RT-PCR方法检测各组三型胶原(ColⅢ)、层粘连蛋白(FN)及CTGFmRNA表达,Western blotting检测各组CTGF、PPAR-γ蛋白表达情况,基于ELISA的DNA-binding检测各组PPAR-γ活性。结果 AngⅡ可增加VSMCs PPAR-γ表达,但明显抑制其活性(P<0.05,vs.Control),PPAR-γ激动剂rosiglitazone可显著增加PPAR-γ蛋白表达及活化(P<0.05,vs.AngⅡ)。rosiglitazone可降低细胞培养液中羟脯氨酸含量,下调VSMCs中ColⅢ、FN、CTGF mRNA表达,抑制CTGF蛋白表达,而其他PPAR-γ激动剂(pioglitazone、15-d-PGJ2)均有相似作用,PPAR-γ拮抗剂GW9662及BADGE可拮抗这一作用。结论在VSMCs中,PPAR-γ激活可抑制AngⅡ诱导的CTGF表达,同时抑制ECM沉积。提示PPAR-γ可能在血管纤维化中起重要作用。     

乡村公路拓宽升级工程软基处理技术措施

拓宽路基的工后不均匀沉降是导致乡村公路升级扩建工程相关病害的主要原因之一,选择合理的路基处理方案可有效的减小拓宽路基工后差异沉降的产生。以蓟县一线穿路拓宽工程、下窝头洼区路拓宽工程为例,选取不同的典型断面,对不同的软基处理方式进行了阐述,并对各典型断面的处理措施成效进行了分析,发现拓宽后的路基固结效果良好、使用状况完全满足设计要求。     

大跨度混凝土梁桥的合理成桥状态设计方法

针对采用节段悬浇施工的大跨度混凝土梁桥普遍存在的持续下挠问题,提出基于荷载效应平衡的"合理成桥状态"概念以及相应的预应力配筋设计方法。通过合理的纵向预应力配置去适度平衡多种作用效应,使桥梁处于合理的成桥状态(弯曲状态、应力状态和挠曲状态),以便能够抑制跨中长期下挠。此外,还提出控制最大压应力水平和采用体内-体外混合配束的设计策略。结合持续下挠的机理讨论,认为合理成桥状态是一种能有效抑制桥梁下挠的实用设计方法。     

基于影响矩阵的桥梁合理设计状态的确定

在分析基于线性、非线性调值计算的影响矩阵法的基础上,深入剖析影响矩阵法在斜拉桥、系杆拱桥、自锚式悬索桥及预应力混凝土梁式桥合理成桥状态确定中的应用,提出一些较具实用价值的计算方法,可为桥梁设计提供有益的参考。     

IFN-γ对兔盆腔术中照射后直肠组织TGF-β1 mRNA含量的影响

目的 探讨IFN-γ对兔盆腔术中照射后直肠组织的病理变化和转化生长因子-β1(TGF-β1)含量的影响.方法 54只雌性新西兰大白兔随机分成单纯照射组、药物干预组和空白对照组,盆腔术中照射后当天开始每隔4 d给予药物干预组肌注IFN-γ 2.5×104u/(kg·f).共5次;于照射后第4、8、12、16、20周每组分别处死5只兔,空白对照组于实验完成时统一处死,光镜下分别观察直肠组织的病理变化,原位杂交法检测TGF-β1 mRNA的表达变化.结果 光镜下观察直肠组织病理改变及原位杂交TGF-β1 mRNA含量,对比药物干预组与单纯照射组和空白对照组间均有显著性差异(P<0.05).结论 IFN-γ能明显抑制TGF-β1 mRNA的表达,可减轻早期放射性炎症反应及后期放射性纤维化的程度.     

Construction of lentivirus vectors carrying alphastatin gene and its secretion expression in human umbilical vein endothelia cells

Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.     

土工合成材料及其在道路路基加固中的应用探讨

土工合成材料是以人工聚合物为原料制成的土工产品,是岩土工程中应用的合成材料的总称,也是当今岩土工程领域中被广泛采用的一种建筑材料,在道路工程中被广泛地应用于道路路基处理。该文针对土工合成材料的分类、特性、应用情况、设计计算进行了探讨与阐述。     

狮子洋隧道地下连续墙施工技术

狮子洋隧道的地下连续墙大部分处于砂层和淤泥质地层中,为了防止槽壁坍塌,通过在实践中的摸索与总结,找到合适性能指标的泥浆。1 000 mm厚的地下连续墙入岩深度较大,为了提高了成槽效率,采用液压抓斗成槽机与冲桩机结合的施工方案,效果明显。     

电针对糖尿病大鼠学习记忆及海马结缔组织生长因子表达的影响

目的 观察电针对糖尿病性认知功能障碍大鼠学习记忆的改善作用及其对海马结缔组织生长因子(connective tissue growth factor, CTGF)mRNA和蛋白表达的影响.方法 采用腹腔注射20g/L链脲佐菌素(streptozotocin, STZ)构建糖尿病大鼠模型,将模型大鼠随机分为电针组(EA)、模型组(DM)、正常组(CN).电针4周后测定大鼠血糖水平,应用Morris水迷宫法观察电针对糖尿病大鼠学习记忆的影响,并用RT-PCR和免疫组化方法检测海马CTGF mRNA和蛋白表达水平.结果 模型组血糖、上台潜伏期高于正常组和电针组(P＜0.01),模型组CTGM mRNA和蛋白表达明显低于正常组和电针组(P＜0.01).结论 电针能在一定程度上降低血糖,促进海马CTGF mRNA和蛋白表达,改善糖尿病认知功能障碍者的学习记忆和认知功能.     

Glutamate enhances the expression of vascular endothelial growth factor in cultured SD rat astrocytes

Objective To study the effect of glutamate on the expression of vascular endothelial growth factor (VEGF) mRNA and protein in cultured rat astrocytes. Methods Cultured rat astrocytes were randomly divided into 6 groups: control group (C), glutamate group (G), QA group (Q), DCG-IV group (D), L-AP4 group (L) and glutanmte-FMCPG gronp (G+M). Cells were cultured under nomoxic condition (95% air, 5% CO2). RT-PCR and ELISA methods were used to detect the expression of VEGF mRNA and protein in cultured astrocytes, respectively. G+ M group was preincubated with lmM MCPG for 30 min prior to the stimulation with glutamate. There were 7 time points at 0,4,8,12,16,24 and 48 h in each group except G+M group. Results The expression of VEGF mRNA and protein did not differ significantly among D group, L group and C group. Different from that in C group, the expression of VEGF mRNA and protein could be enhanced both in a dose-dependent and time-dependent manner in G group and Q group. Meanwhile, the enhanced expression of VEGF mRNA and protein in G group was completely suppressed by MCPG after 24 h. Conclusion Glutamate can increase the expression of VEGF mRNA and protein in cultured astrocytes, which may be due to the activation of group I metabotropic glutamate receptors in astrocytes.