TSH RECEPTOR GENETIC ALTERATIONS IN THE AUTONOMOUSLYFUNCTIONING THYROID ADENOMAS

Objective To determine the relationship between TSH receptor gene mutations and autonomously functioning thyroid adenomas (AY‘]rAs). Methods The thyroid samples from 14 cases of diagnosed AFTAs were analyzed, with normal thyroid specimens adjacent to the tumors as controls. The 155 base pairs DNA fragments which encompassed the third cytoplasmic loop and the sixth transmembrane segments in the TSH receptor gene exon 10 were amplified by Polymerase chain reaction (PCR) and analyzed by the single-strand conformation polymorphism (SSCP). Direct sequencing of the PCR products was performed with Prism Dye Terminator Cycle Sequencing Core Kit.Results 6 of 14 AFTA specimens displayed abnormal migration in SSCP analysis. In sequence analysis of 3 abnormally migrated samples, one base substitution at nucleotide 1957 (A to C) and two same insertion mutations of one adenosine nucleotide between nucleotide 1972 and 1973 were identified. No mutations were found in controls. Conclusion This study confirmed the presence of TSH receptor gene mutations in AFTAs; both one-point substitution mutation and onebase insertion mutation were found to be responsible for the pathogenesis of AFTAs.     

Clinical Detection and Feature Analysis on Neuro Signals

Research on neuro signals is challenging and significative in modern natural science. By clinical experiment, signals from three main nerves (median nerve, radial nerve and ulnar nerve) are successfully detected and recorded without any infection. Further analysis on their features under different movements, their mechanics and correlations in dominating actions are also performed. The original discovery and first-hand materials make it possible for developing practical neuro-prosthesis.     

神经模糊系统在船舶危险度中的应用

在海上交通工程学的最新研究成果的基础上，提出了用一种神经模糊系统计算船舶碰撞危险度的方法．利用人工神经网络的优势，将模糊系统构造成一种神经网络系统，可有效地解决船舶智能避碰系统中模糊规则难以获得的问题．通过实验仿真，系统获得了较为满意的数据．     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

重组人BDNF对Alzheimer病模型神经元作用的实验研究

目的　探讨重组人脑源性神经营养因子 (rhBDNF)对正常原代培养海马神经元及Alzheimer病 (AD)模型海马神经元的作用。方法　预先加入rhBDNF并将 2 0 μmol·L-1的Aβ2 5～ 3 5作用于培养的海马神经元 ,进行形态学观察、胆碱酯酶 (ACHE)组织化学染色、激光共聚焦显微镜及MTT自动比色微量分析。结果　实验对照组海马神经元存活率降至 5 9.5 % ,与空白对照组有显著性差异 (P <0 .0 1)。rhBDNF实验组海马神经元存活率达到 65 .7%～ 72 .6% ,各实验组与实验对照组有显著性差异 (P <0 .0 5 ) ,且 5 0ng·mL-1为rhBDNF最适浓度。实验组胆碱能神经元数量增多 ,胞体直径及突起长度较对照组明显增大 ,其 [Ca2 +]i 相对稳定。结论　rhBDNF对正常培养海马神经元有营养作用 ,能延长细胞生存时间 ;对AD模型海马神经元有保护作用 ,显著降低细胞死亡率 ,抑制了Aβ对神经元的毒性 ,有助于AD的防治     

肺癌患者神经肌肉传递功能的变化

通过重复神经刺激，检查了35例肺癌患者神经肌肉传递、运动神经传导速度（MCV）、感觉神经传导速度（SCV）及感觉神经电位（SNAP）幅度的改变。发现8例燕麦细胞癌患者在重复低频刺激时CMAP幅度减小，重复高频刺激时CMAP幅度增大，5例非小细胞肺癌患者也有同样变化。肺癌患者正中神经和腓肠神经的SNAP幅度明显降低。提示肺癌时既有神经肌肉传递功能的障碍，也有其它的周围神经系统病变。     

新乌鞘岭隧道岭脊段高地应力软岩大变形控制技术

针对新乌鞘岭隧道岭脊段高地应力软岩变形问题,采用加强初支、铣挖法施工在软岩大变形方面取得了良好的效果。通过调整仰拱曲率、加大变形预留量、加强初期支护、超前预加固等设计支护措施,结合铣挖法开挖可有效减少对周边围岩的扰动,有效控制软岩塑性区的发展。研究表明：相比钻爆法,采用铣挖法开挖易于控制超欠挖,并大幅度降低对围岩的扰动。轮廓平均线性超挖由37 cm缩减至17 cm,超挖率由121%缩减至61%,喷射混凝土与岩面密贴性好,有利于提升围岩自承能力和初期支护的承载能力,可有效改善变形控制效果,提高软岩施工的进度及安全性,可作为高地应力软岩大变形控制的有效方法。     

锰神经毒性的病理生理学研究

慢性锰暴露可能引起多巴胺能功能选择性障碍、谷氨酸能运输系统受损、活性氧产生、＂外周型＂苯二氮卓类受体结合位点增加、参与糖分解的三磷酸甘油醛脱氢酶及其它酶类代谢异常,导致能量代谢变化、细胞形态改变、神经元损伤及细胞外谷氨酸浓度增加,这对于从病理生理学角度阐明锰神经毒性机制具有十分重要的作用。     

OPTIMIZATIONS FOR 5-AMINOLEVULINIC ACID BASED PHOTODYNAMIC THERAPY IN PURGING LEUKEMIA CELL HL60

Photodynamictherapy(PDT),whichinvolves administrationofatumorlocalizing photosensiti zingagentthatmayrequiremetabolizingsynthesis(i.e.,aprodrug),followedbyactivationofthea gentbylightofaspecificwavelength.Thistherapy resultsinasequenceofphotochemicalandph…     

EXPRESSION OF MATRIX METALLOPROTEINASE-2 AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN HUMAN GLIOMA AND THEIR RELATION TO THE INVASION OF THE TUMOR

Neovascularizationcanbeobservedinthe courseoftumorformationandmalignantinvasion,whichwillpromotethemalignantinvasionofthe tumor[1].Vascularendothelialgrowthfactor(VEGF)andmatrixmetalloproteinases(MMPs)are theimportantadjustiveproteinsintheinvasionand neov…