D-山梨醇清除率法评估肝储备功能

目的探讨D-山梨醇肝脏清除率(CLh-s)法补充吲哚箐绿15min潴留率(ICGR15)和Child-Pugh分级相结合评估肝硬化门脉高压患者肝储备功能的可行性。方法41名肝硬化门脉高压患者分别给予Child-Pugh分级、ICGR15、CLh-s检测评估肝储备功能,对前两种评估方法出现交叉重叠的患者,进一步分析其CLh-s值与手术并发症的相关性。结果Child-Pugh分级与ICGR15值(以40%为界)交错重叠主要集中在B级和C级之间。在Child-Pugh分级B级的18例患者中,>40%的患者有5例,术后3例出现明显并发症(CLh-s分别为629.52、509.79、450.71 mL/min),2例未出现明显并发症(CLh-s分别为784.49、779.36 mL/min);在Child-Pugh分级C级的13例患者中,有4例ICGR15<40%的患者,2例行手术治疗,其中1例出现并发症(CLh-s为484.57 mL/min)。1例未出现明显并发症(CLh-s为723.36 mL/min)。概括之,CLh-s>700 mL/min者,手术并发症少;CLh-s<700 mL/min,者手术并发症多。结论CLh-s与肝硬化门脉高压患者手术并发症有关。该法可对ICGR15和Child-Pugh分级联合评估肝储备功能时出现交叉重叠者进一步分类,使肝储备功能评估更为准确可靠。     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

siRNA AGAINST SURVIVIN SUPPRESSES THE PROLIFERATION OF PANCREATIC CANCER CELL PC-2 AND INDUCES ITS APOPTOSIS

Survivinis a member of the inhibitor of apop-tosis family,whichinhibits cell apoptosis and regu-lates cell division.Survivin is expressed in embry-onic tissues as well as in the majority of humancancers,but is not expressed in most nor mal adulttissues.The highly cancer-specific expression ofSurvivin,coupled withits i mportance in inhibitingcell apoptosis and in regulating cell division,makesit a useful diagnostic marker and ideal target forcancer treat ment[1].RNAi is a process of post trans…     

清热祛湿方对肝纤维化大鼠TGF-β1蛋白表达的影响

清热祛湿方是我们长期应用于临床治疗慢性肝病的有效方剂。既往通过实验研究发现清热祛湿方有较好的防治肝纤维化作用。为了进一步了解该方防治肝纤维化的作用机制 ,我们采用四氯化碳法建立肝纤维化大鼠模型 ,观察该方对大鼠肝组织中转化生长因子 β1(transforminggrowthfactorbetal,TGF β1)蛋白表达的影响。1　材料与方法1.1　材料　动物 :雄性Sprague Dawley大鼠 4 8只 ,清洁级 ,体重 (2 4 0± 30 ) g ,由西安交通大学医学院实验动物中心提供 ,陕医动证字 :0 8 0 0 5号。药品与试剂 :清热祛湿方 (由飞天蜈蚣七 30g、汉防己 15 g组成 ) …     

白藜芦醇治疗重症急性胰腺炎的实验研究

目的　探讨白藜芦醇对大鼠重症急性胰腺炎炎症介质的作用。方法　应用逆行胰胆管穿刺注射牛黄胆酸钠制备重症急性胰腺炎大鼠模型。90 只大鼠随机分为假手术组、重症急性胰腺炎模型组(SAP)、白藜芦醇治疗组I(RES1,5mg/kg)、白藜芦醇治疗组II(RES2,10mg/kg)、白藜芦醇治疗组III(RES3,20mg/kg)等5 组,每组18 只。术后3、6、12h各剖杀6只,观察重症急性胰腺炎大鼠模型腹水的性质、量,测定血清肿瘤坏死因子、白细胞介素6 的水平,观察治疗组和模型组生存率的变化。结果　白藜芦醇治疗组与重症急性胰腺炎模型组相比,血清中肿瘤坏死因子、白细胞介素6的水平明显下降,死亡率降低,腹水量减少(P<0.05)。结论　白藜芦醇降低重症急性胰腺炎大鼠死亡率,减少腹水量可能与抑制血清肿瘤坏死因子及白细胞介素6的水平有关。     

Survivin、MMP-2、TIMP-2在宫颈癌中的表达及其与侵袭、转移的关系

目的　探讨生存素(Survivin)、基质金属蛋白酶2(MMP 2)、基质金属蛋白酶抑制剂2(TIMP 2)蛋白在人宫颈癌中的表达及其与宫颈癌组织侵袭、转移的关系。方法　采用免疫组织化学S P法和图像分析系统检测Survivin、MMP 2及TIMP 2在10例正常宫颈组织、10例宫颈原位癌、40例宫颈鳞癌和11 例宫颈腺癌中的表达,分析表达结果与临床病理特征的关系。结果　从正常宫颈上皮→原位癌→浸润癌,Survivin、MMP 2 阳性表达量显著升高(P<0.05),TIMP 2的表达与病理分级无关。宫颈癌Survivin、MMP 2、TIMP 2表达量与盆腔淋巴结转移、局部侵袭、组织学类型及患者年龄有关(P<0.05),而与FIGO分期、组织学分级无关(P>0.05)。有盆腔淋巴结转移、有脉管或/和间质侵袭、年龄小于35岁者Survivin、MMP 2阳性表达量增多,而TIMP 2阳性表达量减少(P<0.05);腺癌Survivin阳性表达量高于鳞癌,而其MMP 2、TIMP 2的表达则低于鳞癌(P<0.05)。结论　Survivin、MMP 2和TIMP 2蛋白异常表达在宫颈癌恶化、侵袭和转移中起重要作用, 联合检测这些指标可以预测宫颈癌组织的侵袭和转移能力。     

生存素和CD44V6蛋白在胃癌中的表达及其相关性

目的探讨生存素（survivin）和CD44v6蛋白在胃癌中的表达及意义。方珐应用免疫组化技术检测120例胃癌（GC）、30例异型增生（GED）和20例正常胃黏膜组织（NGM）中survivin和CD44v6蛋白表达，并结合肿瘤的病理学行为和临床随访资料进行分析。结杲在胃癌组织中，survivin和CD44v6阳性率分别为75．8％和81．7％，均显著高于GED和NGM（P〈O．05）；survivin和CD44v6表达与GC浆膜浸润、淋巴结转移和患者预后密切相关（P〈0．05）。结论Survivin和CD44v6表达与GC发生、转移和患者生存期有关，检测survivin和CD44v6蛋白表达可作为预测胃癌患者预后的参考指标。     

MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

Objective To examine the differentially expressed invasion-related genes in two anchorage-independent uterine cervical carcinoma cell lines derived from the same patient using a cDNA array. Methods Two human uterine cervical carcinoma subclonal cell lines CS03 and CS07 derived from a single donor line CS1213 were established by limited dilution procedure. The two cDNA samples retro-transcribed from total RNA derived from CS03 and CS07 cells were screened by a cDNA microarray carrying 234 human cell-cycle related genes and 1011 human signal transduction and membrane receptor -associated genes, scanned with a ScanArray 3000 laser scanner. Results The cDNA microarray analysis showed that ]2 genes in CS03 were up-regulated compared to CS07, and 24 genes in CS07 were upregulated. The function of a number of differentially expressed genes was consistently associated with cell-cycle, cell proliferation, migration, apoptusis, signal transduction and tumor metastasis, including p34^cdc2, TSC22, plasminogen activator inhibitor Ⅰ （PAI-1）and desmusome associated protein（Pinin）. Conclusion Multiple genes are differentially expressed in uterine cervical carcinoma cell lines even came from the same patient. It is suggested that these genes are involved in the different phenotypic characteristics and development of cervical carcinoma.     

DETERMINATION OF URINE TUMOR NECROSIS FACTOR, IL-6, IL-8 AND SERUM IL-6 IN PATIENTS WITH HEMORRHAGIC FEVERS WITH RENAL SYNDROME

Objective To explore the roles of cytokines in the pathogenesis of hemorrhagic fever with renal syndrome（HFRS）. Methods Double-antibody sandwich ELISA was used to determine serum interleukin （IL）-6, urine tumor necrosis factor （TNF）, IL-6 and IL-8 levels in 56 patients with HFRS. Results Serum IL-6, urine TNF, IL-6 and IL-8 concentrations in HFRS patients were significantly higher than those in control group, respectively （P〈0. 001）. The concentrations increased at fever stage, then continued to increase during hypotension stage and peaked at oliguria stage. The concentrations of serum IL-6, urine TNF, IL-6 and IL-8 increased in accord with the severity of the disease and differed greatly among different types of the disease. Serum IL-6 had remarkable relationships with serum specific antibodies. It was positively related to serum β2 -microglobulin （β2 -MG）, blood ureanitrogen （BUN） and creatinine （Cr）. Significant positive relationships were also found both between urine IL-6 and TNF, and between IL-6 and IL-8 （r=0.5768, P〈0.05; r=0. 3760, P〈0.01）. Conclusion TNF, IL-6 and IL-8 activated during the course of the disease. IL-6 is associated with the immunopathological lesions caused by the hyperfunction of humoral immune response. IL-6, IL-8 and TNF are involved in the renal immune impairment. Determining them might, in certain extent, be used in predicting the prognosis and outcome of patients with HFRS.     

蓖麻毒蛋白糖脂脂质体对肝癌细胞的杀伤作用及其 …

目的 探索一种疗效显著的新型肝癌介入治疗新药，对蓖麻毒蛋白糖脂脂质体包封物进行有关实验研究。方法 以中药蓖麻子的提取物－蓖麻毒蛋白与糖脂脂质体－半乳糖神经酰胺进行包封后形成的蓖麻毒蛋白糖脂脂质体包封物（Ｒ－ＧＣＬ）作为设计药物进行实验研究，了解包封后的蓖麻毒蛋白对肝癌细胞的杀伤力。结果 在体外实验中，当Ｒ为０．０５５μｇ／ｍｌ时，游离蓖麻毒蛋白作用后肝癌细胞的存活率为３８．５％，Ｒ－ＧＣＬ为２８．