实验鼠脑组织对新型光敏剂NPe6吸收的荧光研究

目的 测量了新型剂ＮＰｅ６在白鼠（ＢＡＬＢ／Ｃ，雄性）脑组织中的吸收情况，为减少光动力学疗法对正常脑组织的损伤提供关键的实验数据，方法 首次采用了激光诱导荧光产额测量的方法，测得ＮＰｅ６在脑组织中的吸收情况，并观测激光照射下给予ＮＰｅ６和未给予组鼠的不同反应。结果 实验测量光敏剂ＮＰｅ６在鼠脑组织的浓度随时间变化的情况，与已报道的同位素标记方法所得结果一致，观测了激光照射下给药和未给药组鼠的不同反     

铁路电力牵引工频电磁场对人体的健康影响与防护对策

电气化铁路因27.5 kV电力牵引接触网周围形成工频（50 Hz）电磁场广泛存在于铁路运营的多个环节（场所）,对电气化铁路职工造成职业危害。本文就工频电磁场对人体免疫系统的影响、遗传毒作用及其与肿瘤和白血病的关系等进行综述,旨在为提出电气化铁路职工的工频电磁场防护对策、开展电气化铁路职业卫生工作提供科学依据。     

In situ absorption and metabolism of stilbene glycoside in rat

Stilbene glycoside (TSG) has been shown to have many beneficial properties. It is therefore essential to understand the absorption and metabolism of TSG in detail. We determined the recovery of TSG and its metabolites (TSG sulfate/glucuronides) in rat gastric contents, gastric mucosa, portal vein plasma, celiac arterial plasma, bile, and urine after administration of 15mg of TSG in 0.5mL physiological saline or incubation for 20min in situ in the stomach of rats. Within 20min, (64.0±9.8)% of the administered TSG disappeared from the stomach; later, TSG was recovered in both free and conjugated forms in plasma and bile, but not in urine. On the other hand, only free TSG was detected in the gastric contents and mucosa; it was also detected in the portal vein plasma as (48.1±3.5)% of the total TSG (all forms of TSG). However, the proportion of free TSG in the celiac arterial plasma and bile decreased to 4%-10%. In addition, the proportion of free TSG to total TSG in the liver microsome incubation mixture after TSG was incubated in liver microsome at 37℃ for 30min was very low [(10.6 ± 2.6)%]. These results indicate that TSG could be quickly absorbed from the rat stomach, conjugated in liver and excreted in bile. Such novel information would be helpful for the use of TSG as a beneficial natural product which may improve its proposed efficacy in preventing chronic diseases.     

热带风暴中落海后船员血清细胞因子的观察

目的 观察在热带风暴条件下落海１８小时后的船员血热酶含量（或活性）、钙磷浓度、ＴＮＦα、ＩＬ－１２＾２（Ｐ＾７０）含量的变化。方法 对３１名落水受海浸泡的船员于入院后第１天、第８天血清、尿液、外周血样本进行检测（称船员组）,另取３４史休检健康者的样本作为对照检测（称对照组）。血清ＣｕＺｎ－ＳＯＤ、ＴＮＦα及ＩＬ－２含量测定用ＲＩＡ法;ＣＫ、ＣＫ－ＭＢ、ＬＤＨ及α－ＨＢＤＨ活性测定用ＣＯＢＳＡ ＦＡ     

经颈内静脉肝内门体分流术治疗门脉高压

评价经颈内静脉肝内门体分流术治疗门脉高压症的应用价值。方法采用ＲＵＰ－１００穿刺导管系统对１０例门脉高压患者经右颈内静脉宽刺，建立静脉脉和肝静脉之间肝内通道后置入血管同人支架。     

斜坡肿瘤手术入路的选择

报告3例巨大斜坡肿瘤伴有球麻痹及不全高位截瘫,分别采用经颞枕底,经口咽,经额颅底斜坡入路施行全或次全切除肿瘤,效果良好,平均随访2.8年。如何根据每个病例选择好手术入路,是决定手术成功的关键。     

PKM2对肝内胆管癌细胞增殖及上皮间质化的影响

目的 检测M2-型丙酮酸激酶(pyruvate kinase M2,PKM2)在肝内胆管细胞癌(intrahepatic cholangiocarcinoma,ICC)组织中的表达情况,探讨PKM2对ICC细胞增殖及上皮间质化(EMT)的影响.方法 RT-PCR和免疫组化法检测ICC组织中PKM2的表达,慢病毒载体介导...     

甲状腺癌患者血清中SIL-2R测定的临床意义

测定 59例甲状腺良、恶性疾病患者血清中可溶性白介素 受体 (SIL- 2 R)。结果显示 :甲状腺癌患者血清中 SIL- 2 R明显高于良性疾病组及对照组 (P <0 .0 1 )。良性腺瘤及结节性甲状腺肿与对照组间无明显差异 (P >0 .0 5)。甲状腺癌术后 5～ 1 0 d血清中 SIL- 2 R较术前增高 ,可能与术前血清中 SIL- 2 R高水平及手术干扰机体免疫有关。术后约 2 0 d基本降至正常。甲状腺癌血清中 SIL- 2 R升高与甲状腺癌的病理类型无明显关系。提示检测血清中 SIL- 2 R对判别甲状腺疾病良、恶性 ,估计疗效及预后有一定临床意义     

HGV与HBV、HCA重叠感染病例的研究

探讨庚肝病毒 ( HGV)与 HBV、HCV重叠感染状况。方法　采用套式逆转录聚合酶链反应 ( RT- n PCR)技术检测了 2 2例乙肝病毒感染者及 48例丙肝病毒感染者血清 HGVRNA。结果　 HGV与 HBV、HCV重叠感染率分别为 31 .8% ( 7/ 2 2 )、31 .3% ( 1 5/ 48) ,重叠感染病例与单纯 HBV、HCV感染者在 ALT、SBIL水平及疾病的临床分型上无统计学差异。结论　HGV与 HBV、HCV重叠感染的几率均等 ,HGV似不加重乙肝及丙肝患者的病情     

自动水模体放射剂量测量装置电控系统的设计与实现

研究了一种新式的、基于数控技术的自动水模体测量装置电控系统,阐述了该电控系统的组成结构,重点介绍了系统硬件的实现方法以及软件的模块化设计.通过试验已经证明该系统具有良好的实际应用效果.