人鼻咽癌放射抗拒性肿瘤干细胞的分离、筛选与鉴定

目的分离、筛选、鉴定人鼻咽癌CNE-2及放射耐受CNE-2R的肿瘤干细胞。方法免疫磁珠技术分选CNE-2及CNE-2R中鼻咽癌CD133+干细胞,分别用无血清培养法、平板克隆法、裸鼠成瘤试验检测分选的CD133+细胞体外增殖能力、克隆形成能力及成瘤能力,HE染色和电镜超微结构观察形态学变化。结果 CNE-2及CNE-2R细胞中CD133+表达分别为(1.79±0.16)%和(2.63±0.72)%;CNE-2-CD133+细胞及CNE-2R-CD133+细胞形成肿瘤干细胞球及体外增殖能力远高于CD133-表达者;CNE-2R-CD133+细胞克隆形成能力较CNE-2-CD133+细胞明显升高,而且CNE-2R-CD133+细胞的克隆形态与CNE-2-CD133+细胞相比较明显增大;形态学发现CNE-2R-CD133+细胞核大,异型,核仁大,细胞器肥大。结论分离、筛选出的鼻咽CNE-2R-CD133+肿瘤干细胞能形成干细胞球,其增殖能力、裸鼠成瘤能力更强,能为进一步探讨鼻咽癌放射抗拒的机制提供研究基础。     

126例涎腺肿瘤的免疫组织化学研究

利用S-100蛋白抗体对126例涎腺肿瘤（多形性腺瘤、恶性多形性腺瘤、粘液表皮样癌、腺样囊性癌、基底细胞腺瘤和乳头状囊腺瘤等）进行了免疫组化研究。结果发现:5种肿瘤中都有瘤细胞或部分瘤细胞表达S-100蛋白;但乳头状囊腺瘤的肿瘤性上皮则呈S-100蛋白阴性反应。提示:S-100蛋白抗原在涎腺肿瘤性肌上皮细胞中均有表达。     

TSH RECEPTOR GENETIC ALTERATIONS IN THE AUTONOMOUSLYFUNCTIONING THYROID ADENOMAS

Objective To determine the relationship between TSH receptor gene mutations and autonomously functioning thyroid adenomas (AY‘]rAs). Methods The thyroid samples from 14 cases of diagnosed AFTAs were analyzed, with normal thyroid specimens adjacent to the tumors as controls. The 155 base pairs DNA fragments which encompassed the third cytoplasmic loop and the sixth transmembrane segments in the TSH receptor gene exon 10 were amplified by Polymerase chain reaction (PCR) and analyzed by the single-strand conformation polymorphism (SSCP). Direct sequencing of the PCR products was performed with Prism Dye Terminator Cycle Sequencing Core Kit.Results 6 of 14 AFTA specimens displayed abnormal migration in SSCP analysis. In sequence analysis of 3 abnormally migrated samples, one base substitution at nucleotide 1957 (A to C) and two same insertion mutations of one adenosine nucleotide between nucleotide 1972 and 1973 were identified. No mutations were found in controls. Conclusion This study confirmed the presence of TSH receptor gene mutations in AFTAs; both one-point substitution mutation and onebase insertion mutation were found to be responsible for the pathogenesis of AFTAs.     

肝动脉,门静脉双介入化疗治疗肝癌的研究

对４７例原发性肝癌患者采用皮下泵行双介入治疗，即对肝动脉栓塞化疗和门静脉灌注化疗，肝动脉门静脉双灌注化疗，临床结果表明：无论在临床症状改善，肿瘤缩小程度，治疗的有效还是延长生存期方面，栓塞组均比化疗组显著，而肝癌切除后局部预防组对肝癌复发的预防比周围预防组疗效显著，该方法操作简便，易于掌握，并发症少，提供了一条治疗肝癌的有效途径。     

SURVEY SUBAXIAL CERVICAL VERTEBRAE FOR TRANSPEDICULAR SCREW FIXATION

Therearemanycausesofsubaxialcervicalinstabilitysuch astrauma,degenerativedisease,neoplasm,andinfection.Therearenumerousinternalfixationproceduresthathavebeen usedforone stageposteriordecompressionandstabilizationof thecervicalspine.Recentlydevelopedposteriorplatescrew fixationprocedureshavebeenperformedinthecervicalspine byusingalateralmassscreworapediclescrewformal stage posteriordecompressionandstabilizationofthecervical spine[1].Theseveralpotentialrisksofneurovascularcomplica tions,whichare…     

人参皂甙20(R)-Rg_3治疗肺癌的临床疗效观察

~~人参皂甙20(R)-Rg_3治疗肺癌的临床疗效观察@陈明伟$西安交通大学第一医院呼吸内科!陕西西安710061 @陈健$西安交通大学第一医院呼吸内科!陕西西安710061 @姚煜$西安交通大学第一医院呼吸内科!陕西西安710061 @石志红$西安交通大学第一医院呼吸内科!陕西西安710061新药研究;;人参皂甙;;肺肿瘤~~     

THE INHIBITORY EFFECT OF MELATONIN ON THE GROWTH OF HUMAN BLADDER CARCINOMA T24 CELL LINE

Melatonin(Mel) ,anindoleamineprimarilyderivedfromthepinealgland ,issecretedduringthehoursofdarkness.Melactsasahormonalconduc tionof photoperiodinfluencedbytheseasonanddailyphysiologicalrhythms.Thefunctionofmelinhumansisobscure .However ,melhasattainedprominenceasatreatmentfordisturbedcircadianrhythmsandsleeppatterns ,hasenhancedthefunc tionofimmunitysystem[1] andhasinhibitedtumorgrowth[2 ] .Thepreviousstudieshaveindicatedthatmelcombinedwithotheranticancerdrugscanin creasetheefficiencyanddecre…     

中药内异消对大鼠子宫内膜异位症血管生成因子的影响

目的　探讨血管内皮生长因子 (VEGF)、肿瘤坏死因子 (TNF α )在子宫内膜异位症 (EM)发病机制中的作用及相互关系 ,观察中药内异消对两因子的影响及对EM的治疗作用。方法　采用种植内膜方法建立EM动物模型 ,内异消治疗 ,丹哪唑对照 ,ELISA法测定TNF α水平 ,免疫组化SP法检测VEGF表达。结果　EM病模组异位内膜组织中VEGF的表达及腹腔液TNF α含量显著增高 ,与假模型组比较有统计学差异 (P <0 .0 1) ;内异消能降低VEGF表达及TNF α含量 ,与病模组比较有统计学差异 (P <0 .0 1)。结论　VEGF、TNF α在EM发生发展中起重要作用 ,内异消可通过调节两因子的表达来抑制异位内膜的生长     

P53和Sialyl Lewis-X表达与甲状腺乳头状癌侵袭转移的关系

目的　探讨甲状腺乳头状癌(PTC)组织中P53和唾液酸化路易斯- X(SLeX)蛋白表达与肿瘤侵袭、转移的关系。方法　应用免疫组织化学催化信号放大(CSA)方法,对46例PTC、20例结节性甲状腺肿(NG)、20例慢性淋巴细胞甲状腺炎(CLT)和15例正常甲状腺组织(NT)进行了 P53 和 SLeX基因蛋白检测。结果　在 PTC中,P53 和 SLeX表达阳性率分别为65.2%(30/46)和69.6%(32/46),显著高于 NG、CLT和 NT(P<0.05);P53 和 SLeX表达阳性与PTC的侵袭、转移呈显著正相关(P<0.05)。结论　P53 和 SLeX阳性表达与 PTC侵袭、转移密切相关,检测 P53 和SLeX蛋白表达可作为判断PTC预后的参考指标。