实时荧光定量PCR检测宫颈癌组织中HGF mRNA和c-Met mRNA的表达

目的检测宫颈癌组织中肝细胞生长因子(HGF)及其受体原癌基因c-Met的分子表达水平,并探讨其临床意义。方法采用实时荧光定量PCR(QRT-PCR)技术检测43例宫颈癌,30例宫颈上皮内瘤变Ⅲ和27例正常宫颈组织标本中HGF和c-Met的分子表达水平。结果 HGF mRNA和c-Met mRNA在宫颈癌组织中均呈高表达,其与宫颈癌患者临床分期、肿瘤直径大小、浸润深度、淋巴结转移有关(P均<0.05),而与肿瘤的分化程度无关(P>0.05)。HGF mRNA和c-Met mRNA之间存在正相关(r=0.666,P<0.01)。结论 QRT-PCR技术可对宫颈癌组织中的HGF mRNA和c-Met mRNA表达进行定量检测;HGF mRNA和c-Met mRNA的高表达与宫颈癌的侵袭转移有关。两者联合监测有利于早期诊断宫颈癌,为宫颈癌的治疗和预后提供参考。     

Microfluidics-based assay on the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors

Abstract A new microfluidic system with four different microchambers （a circle and three equilateral concave polygons） was designed and fabricated using poly（dimethylsiloxane） （PDMS） and the soft lithography method. Using this microfluidic device at six flow rates （5, 10, 20, 30, 40, and 50 μL/h）, the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors were investigated. Escherichia coli HB101 pGLO, which could produce a green fluorescent protein induced by L-arabinose, was utilized as the model bacteria. The results demonstrated that bacterial adhesion was significantly related to culture time, microenvironment geometry, and aqueous flow rates. Adhered bacterial density increased with the culture time. Initially, the adhesion occurred at the microchamber sides, and then the entire chamber was gradually covered with increased culture time. Adhesion densities in the side zones were larger than those in the center zones because of the lower shearing force in the side zone. Also, the adhesion densities in the complex chambers were larger than those in the simple chambers. At low flow rates, the orientation of adhered bacteria was random and disorderly. At high flow rates, bacterial orientation became close to the streamline and oriented toward the flow direction; All these results implied that bacterial adhesion tended to occur in complicated aqueous flow areas.The present study provided an on-chip flow system for physiological behavior of biological cells, as well as provided a strategic cue for the prevention of bacterial infection and biofilm formation.     

Rapid europium-sensitized fluorescent determination of ulifloxacin,the active metabolite of prulifloxacin,in human serum

A new fluorescent method was developed based on the ulifloxacin-europium（Ⅲ）-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ul     

氙弧灯和荧光紫外灯对面漆漆膜破坏性的比较

依据2项国家标准,分别采用氙弧灯和荧光紫外灯对不同颜色面漆漆膜的破坏性开展试验研究.试验结果显示,2种光源对面漆漆膜破坏性的区别主要表现在失光、色差和粉化方面.从成膜物质和试验条件方面分析试验结果的形成原因,比较2种光源对漆膜破坏的等效性.     

HTK—90型荧光磁粉探伤机磁化参数的整定及相关的单片机中断和定时技术

简单说明ＨＴＫ－９０型荧光磁粉探伤机磁化电流幅度和作用时间的控制方法，介绍了幅度参数和时间参数的鉴定。     

汽油机喷油器光学油盘测试方法研究

在定容弹喷雾试验台上,利用平面激光诱导荧光原理搭建了光学油盘测试研究平台,试验研究了PFI及GDI汽油机喷油器的喷雾油束角度及落点特性,分析了喷雾记录时间段、时间间隔和喷孔数量对测试结果的影响规律。研究结果表明,光学油盘法对不同孔数的PFI喷油器和GDI喷油器都适用,其结果具有普适性。该方法测试数据与传统油盘法数据相近,结果具有较高的分辨率,且比油盘法测试结果更加直观。     

RD2型滚动轴承轮对防尘板座与轮座前肩过渡圆弧部位横裂纹的探伤工艺分析

1问题的提出2005年2月26日,某车辆段在给C62B4601787号敞车做段修时,探伤工用3000型荧光磁粉探伤机检查出该车3位车轴防尘板座与轮座前肩过渡圆弧处存在1条长42 mm、深1·0 mm的横向裂纹。该裂纹距车轴左端面272 mm,有10 mm与车轴轮座前肩重合(见图1)。该车轴由齐齐哈尔铁路车辆(集团)有限责任公司于1 9 8 8年1 2月制造,1 9 8 9年3月2 6日组装。本次段修是因左端轴承到期而将轴承退卸,在对车轴除锈后施行电磁探伤检查时发现了该处横裂纹的存在。然后,分别用轮轴微机控制超声波自动探伤机和图1裂纹部位示意图多通道超声波探伤仪进行对比检测…