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901.
902.
903.
在介绍中机车辆技术服务中心提出的三轴自卸汽车载质量利用系数和货箱栏板高度旧新规定的基础上,结合该类车型的设计生产实例进行深入分析,提出新规定对企业设计制造该类车型时的影响及其在实际运营中的意义。 相似文献
904.
Jiang-Fang Lian Xiao-Yan Huang Wei-Feng Xu Xi Yang Ying Wang Di Li Jian-Qing Zhou 《西安交通大学学报(英文版)》2010,22(3):188-191
Objective To identify the mutation of human ether-a-go-go-related gene (hERG) and analyze the clinical characteristics of a Chinese family with long ST syndrome (LQTS). Methods The electrocardiogram and DNA samples were obtained from a Chinese LQTS family of 26 members. Genotype was performed with polymorphic short tandem repeat (STR) markers at the known LQT1, LQT2, and LQT3 loci. SSCP analysis was used to find aberrant conformers. hERG mutation was confirmed by cloning and sequencing. Results Three gene carriers were linked to chromosome 7q35-36, where the potassium channel gene hERG was encoded. A 19-base pair deletion was identified. The mutation was located at nucleotide position 1 619-1 637 between transmembrane domains S4 and S5. Furthermore, A1692G polymorphism was found both in the normal control and patients. Conclusion A novel 19 bp deletion mutation of hERG is identified in a Chinese family. All gene carriers are demonstrated to be typical LQT2 ECG phenotype. 相似文献
905.
Hui Jin Hai-Tao Hu Wei-Xi Wang Gai-Feng Feng Zhao-Hui Liu Wei-Na Yang 《西安交通大学学报(英文版)》2010,22(2):105-110
Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity. 相似文献
906.
Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer. 相似文献
907.
更年期妇女潮热的相关因素分析 总被引:2,自引:0,他引:2
目的 探讨更年期妇女潮热发生的相关因素,进一步提高更年期妇女的生活质量.方法 对792名更年期妇女进行问卷调查,内容包括一般情况、月经情况、孕产史、潮热情况、日常生活方式和性生活情况,记录身高、体重、血压、腰围和臀围.运用Logistic回归分析,研究与更年期妇女潮热的相关因素.结果 单因素分析显示:年龄、文化程度、经济收入、目前月经情况、饮食习惯、豆制品食用、体格锻炼、性生活满意度、体重指数及收缩压与更年期潮热有关.多因素Logistic回归分析显示:月经不规律或绝经、体格锻炼频繁及收缩压较高为更年期妇女潮热的危险因素,文化程度较高、食用豆制品及性生活满意为保护因素.结论 指导更年期妇女进行适度的体育锻炼,了解更年期相关知识,多食用豆制品,控制收缩压也许可降低潮热的发生. 相似文献
908.
909.
目的 通过测定溃疡性结肠炎(UC)大鼠血清酪酪肽(PYY)水平及其空肠黏膜上皮细胞膜PYY受体的特性来观察UC时血清PYY水平及其受体的变化.方法 将大鼠随机分成UC组、腹泻型肠易激综合征(D-IBS)组及对照组,用同位素放射免疫法分别检测各组的PYY水平及反映受体特性的两个参数:平衡解离常数(Kd)和最大结合容量值(Bmax).结果 UC及D-IBS组血清PYY水平高于对照组(P<0.001),且UC组亦高于D-IBS组(P<0.001);但UC组Kd及Bmax值与正常组和D-IBS组比较均无明显差异(P>0.05).结论 UC组PYY水平高于正常及D-IBS组,推测UC时PYY水平的变化可能与其腹泻症状和炎症病变的产生有关;UC组的Kd及Bmax与正常组比较无显著变化,推测UC可能与PYY受体的变化无关. 相似文献
910.
本文讨论了产油作物脂肪酸相关基因的研究进展,以及克隆和鉴定产油作物脂肪酸脱饱和酶具有的重要理论价值和应用前景,为产油作物的进一步经济开发提供一定的理论依据。 相似文献