国际海事卫星系统-F站模拟器设计

GMDSS全球海上遇险与安全系统（Global Maritime Distress and Safety System）是在现代无线电通信技术的基础上，为适应海上搜救与安全通信，满足海上通信的需要而建立起来的遇险和安全通信系统。而INMARSAT-F站便是一种增强型的海用全球区域网络，它使用增强型新一代信令系统，确保兼容INMARSAT第4代卫星以及新型呼叫优先级划分计划，改善遇险呼叫处理功能。鉴于使用真实的INMARSAT-F费用高而无法进行培训等原因，我们研究设计了INMARSAT-F站模拟器。     

Air–sea CO2 fluxes in the Caribbean Sea from 2002–2004

Air–sea fluxes in the Caribbean Sea are presented based on measurements of partial pressure of CO₂ in surface seawater, pCO_2sw, from an automated system onboard the cruise ship Explorer of the Seas for 2002 through 2004. The pCO_2sw values are used to develop algorithms of pCO_2sw based on sea surface temperature (SST) and position. The algorithms are applied to assimilated SST data and remotely sensed winds on a 1° by 1° grid to estimate the fluxes on weekly timescales in the region. The positive relationship between pCO_2sw and SST is lower than the isochemical trend suggesting counteracting effects from biological processes. The relationship varies systematically with location with a stronger dependence further south. Furthermore, the southern area shows significantly lower pCO_2sw in the fall compared to the spring at the same SST, which is attributed to differences in salinity. The annual algorithms for the entire region show a slight trend between 2002 and 2004 suggesting an increase of pCO_2sw over time. This is in accord with the increasing pCO_2sw due the invasion of anthropogenic CO₂. The annual fluxes of CO₂ yield a net invasion of CO₂ to the ocean that ranges from − 0.04 to − 1.2 mol m^− 2 year^− 1 over the 3 years. There is a seasonal reversal in the direction of the flux with CO₂ entering into the ocean during the winter and an evasion during the summer. Year-to-year differences in flux are primarily caused by temperature anomalies in the late winter and spring period resulting in changes in invasion during these seasons. An analysis of pCO_2sw before and after hurricane Frances (September 4–6, 2004), and wind records during the storm suggest a large local enhancement of the flux but minimal influence on annual fluxes in the region.     

浙江省B2C电子商务企业物流模式探讨

B2C电子商务企业能取得多大的成功很大程度上依赖于实际物流的操作,B2C电子商务企业必须根据自身情况,正确选择和采用企业自营物流模式或是第三方物流模式。本文根据实地调研的数据,就浙江省B2C电子商务企业的物流模式存在的问题进行分析探讨,寻求成本低廉、需求响应快速、安全可靠且适宜的物流模式。     

汽车制动系统试验装置的开发及应用

研究和开发了基于GB12676－1999标准的汽车制动系统试验装置，该试验装置所测量的参数有：车轮的转速、转矩，整车速度，制动管路压力，踏板力，制动蹄片的温度等。为具备这些功能，本装置由传感器部分、信号处理部分、数据采集部分构成。对汽车制动时车轮转矩的测量进行了深入分析，并编制了相应的采集处理软件，进行了系统的实车试验。     

沿河公路丁坝群水毁防护平面二维水流数值模拟研究

从平面二维水动力学方程组出发,根据有限元Galerkin加权余量法离散模型方程组,并利用Newton-Raphson迭代法求解,建立丁坝绕流平面二维水流数学模型。利用水槽试验数据对模型进行了验证,模型计算结果与实测值吻合良好。将模型应用到河龙高速清溪河段水毁防护工程,对丁坝群护岸进行了模拟分析,结果表明,该模型在丁坝群护岸工程的水流模拟中具有推广价值。     

电力机车电子式劈相机起动继电器的改进设计

为了提高电力机车劈相机起动继电器的运用可靠性,分析了目前广泛使用的TJQ2S劈相机起动继电器,在此基础上改进设计了一种双路型劈相机起动继电器,现场测试表明其功能完善,操作简便,有较高的应用价值。     

Modelling Pseudocalanus elongatus stage-structured population dynamics embedded in a water column ecosystem model for the northern North Sea

This paper outlines an approach to couple a structured zooplankton population model with state variables for eggs, nauplii, two copepodites stages and adults adapted to Pseudocalanus elongatus into the complex marine ecosystem model ECOHAM2 with 13 state variables resolving the carbon and nitrogen cycle. Different temperature and food scenarios derived from laboratory culture studies were examined to improve the process parameterisation for copepod stage dependent development processes. To study annual cycles under realistic weather and hydrographic conditions, the coupled ecosystem–zooplankton model is applied to a water column in the northern North Sea. The main ecosystem state variables were validated against observed monthly mean values. Then vertical profiles of selected state variables were compared to the physical forcing to study differences between zooplankton as one biomass state variable or partitioned into five population state variables. Simulated generation times are more affected by temperature than food conditions except during the spring phytoplankton bloom. Up to six generations within the annual cycle can be discerned in the simulation.     

主断路器分合闸速度信号采集系统的设计

介绍了采用单片机和A/D转换器设计主断路器分、合闸速度信号采集系统的方案,同时详细描述了该系统的工作原理及硬件、应用程序的设计。     

新型抗ErbB2、抗CD16三价双特异性抗体的构建及功能鉴定

目的构建一种新型三价抗ErbB2、抗CD16 BsAb。方法构建重组载体pET22b( )/BsAb;转化大肠杆菌BL21(DE3),获得重组蛋白的表达,通过包涵体复性纯化蛋白。应用悬浮培养系统扩增稳定表达抗人CD16 scFv-Fc融合蛋白的CHO细胞株(CG5细胞)和稳定表达抗人ErbB2 scFv-Fc融合蛋白的CHO细胞株(HG2细胞),通过rProtein A Sepharose Fast Flow亲和层析柱纯化融合蛋白,应用流式细胞术分析BsAb的结合能力。结果该BsAb可在大肠杆菌BL21(DE3)中以包涵体形式高效表达,通过对重组蛋白的复性可获得高纯度的蛋白;复性后的BsAb具有与其亲本抗体相似性的结合靶抗原的能力。结论新型三价抗ErbB2、抗CD16 BsAb能与高表达ErbB2的乳腺癌细胞系SKBR3细胞高亲和力结合和表达CD16的人外周血单个核细胞低亲和力结合。     

抵抗素、C反应蛋白与早期2型糖尿病微血管病变的关系

目的 探讨2型糖尿病(T2DM)患者血浆抵抗素、血清C反应蛋白(CRP)水平与早期T2DM微血管病变的相关性.方法 选取60例临床确诊的T2DM患者,按有无微血管并发症分为微血管并发症组(DMA组)和无微血管并发症组(NDC组),每组30例.另选取24例同期体检的健康志愿者作为对照组(NC组).采用双抗体夹心酶联免疫吸附试验测定3组的血浆抵抗素含量,散射免疫比浊法测定血清CRP含量,放射免疫分析法测定胰岛素,氧化酶法测定血糖,全自动生化分析仪检测血脂.结果 ①DMA组血浆抵抗素、血清CRP含量均高于NDC组及NC组,差异有统计学意义(P＜0.05).②Pearson(或Spearman)相关分析显示,血浆抵抗素与空腹胰岛素(FIns)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA-IR)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)呈正相关(r分别为0.582、0.433、0.435、0.342、0.485,P均＜O.05),与空腹血浆葡萄糖(FPG)、甘油三酯(TG)、体质指数(BMI)、高密度脂蛋白胆固醇(HDL-C)均无相关性;血清CRP与尿微量白蛋白(UALb)、血浆抵抗素、HOMA-IR、FIns、HbA1c呈正相关(r分别为0.388、0.240、0.283、0.279、0.318,P＜O.05),与HDL-C呈负相关(r=0.235,P＜O.05).结论 血浆抵抗素、血清CRP可能与2型糖尿病微血管病变的发生、发展有密切关系.