EFFECTS OF ANTISENSE OLIGODEOXYNUCLEOTIDES ON EXPRESSION OF CASPASE-3 IN Γ-RADIATION INDUCED APOPTOTIC HL-60 CELLS

Apoptosisisaprogrammedcelldeaththatis regulatedbyanintracellularpathwayinvolving caspaseenzymes.Apoptosisisessentialtonormalde velopmentandensuresdamagedordysfunctional cellsdestroythemselvesinacontrolledfashion.Dysfunctionofapoptosisresultsinmanyhumandis…     

兔海水淹溺肺水肿肺Na^＋—K^＋—ATP酶和细胞色素氧化酶活性变化？…

目的：探究海水淹溺肺水肿（ＰＥ－ＳＷＤ）的典型症状及其病生理变化，方法：通过向兔肺进行海水灌注诱导ＰＥ－ＳＷＤ，用细胞化学和图像定量分析方法测定兔肺组织Ｎａ＾＋－Ｋ＾＋－ＡＴＰ酶和细胞色素氧化酶（ＣＹＴＯ）活性。结果：ＰＥ－ＳＷＤ组兔肺中，以上两种酶活性显著降低，结论；酶活性的降低可能与淹溺造成肺组织缺氧有关，同时缺氧引起ＡＴＰ减少导致一系列肺组织代谢，功能与结构的变化，造成肺不张和ＰＥ－ＳＷＤ。     

胃肠癌的MHC抗原表达及其与局部免疫应答的关系

利用一组单克隆抗体对62例胃肠癌组织进行免疫组化研究。结果发现:HLA-ABC抗原缺失率在胃癌,结、直肠癌和局部淋巴结转移灶分别为26.92%、13.89%和33.33%;HLA-DR抗原表达率分别为34.62%、22.22%、和40.0%,而HLA-DQ抗原仅表达于2例HLA-DR胃癌的少数癌细胞。且HLA-ABC抗原缺失率在早期癌(60.0%)明显高于中晚期癌(15.79%);胃肠癌的HLA抗原表达与局部单个核细胞各亚群浸润的程度间也有一定的相关。这些发现提示HLA抗原可能在肿瘤发生发展过程中具有重要的免疫调节功能。     

绞股蓝总皂甙对P388细胞的作用及机制探讨

用单层平皿软琼脂克隆法证明绞股蓝总皂甙对体外培养的P388细胞克隆生长有明显的抑制作用，其IC50为（22．5±1．0）mg·L-1。为进一步探讨其抑制P388细胞生长机理，用放射性同位素掺入法观察了绞股蓝总皂成对P388细胞DNA、RNA及蛋白质合成的影响。结果表明，在绞股蓝总皂甙作用24h内，其主要抑制P88细胞DNA合成，轻度抑制RNA合成，而对蛋白质合成几乎无影响。     

Interference and Spectral Cellular Network with Efficiency Analysis in Two-hop Fixed Relays in FDD Mode

This paper presented ascheme of two-hop cellular network with fixed relay nodes （FRN）. Based on this scheme, co-channel interference and signal interference ratio（SIR） received by base station（BS） and FRN were analyzed. Both the theoretical analysis and simulation results show that the SIR can be improved significantly when relays are employed in the network. The higher spectral efficiency can be obtained due to the improved two-hop link quality through the use of adaptive modulation and coding （AMC）. The antenna height of FRN and the cell radius of BS and that of FRN influence SIR received by BS and FRN and the system spectral efficiency greatly. The proper antenna height of FRN and cell radius of BS and that of FRN were also given to get the highest spectral efficiency.     

EPO对早期糖尿病大鼠肾脏的保护作用

目的观察亚临床剂量EPO对糖尿病大鼠早期肾损伤的影响,并探讨其作用机制。方法将大鼠随机分为正常对照组(NC)、糖尿病组(DM)及糖尿病EPO干预的两个剂量亚组(DMTA和DMTB)。采用链脲佐菌素诱导1型糖尿病大鼠模型,腹腔注射EPO10周后,测定血糖、血压、血红蛋白、肾功能;光镜及电镜下观察肾组织形态学变化;免疫组化分析肾组织细胞凋亡相关蛋白Bcl-2/Bax及细胞因子VEGF的表达。结果实验剂量EPO干预后,大鼠肾功能及其形态学指标得到显著改善;Bcl-2/Bax比值及VEGF表达升高;均呈剂量依赖性。血糖、血压、血红蛋白值与对照组相比无明显变化。结论亚临床剂量EPO可延缓早期糖尿病肾病发展进程,可能与上调Bcl-2/Bax蛋白表达比值,抑制肾脏细胞凋亡有关;在本实验条件下,肾组织VEGF的表达上调未对糖尿病肾病造成不良影响。     

Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.     

Mammalin cochlear supporting cells transdifferentiation into outer hair cells

Objective To study the recovery of the outer hair cells in the bat cochlea after gentamicin exposure.Methods Bats were injected with a daily dose of gentamicin for 15 consecutive days and bromodeoxyuridine (BrdU)was given from day 16 to day 40 of this recovery phase. Hearing was assessed by overt acoustic behavior and auditory brainstem responses analysis, which was performed one day prior to the first injection and a day after the last injection (day 16). On day 40 animals were sacrificed for detection of cells that could take up BrdU. Results After 15 days of gentamicin treatment, all of the animals were proved to be deafened with significant increases of ABR thresholds,compared with control group. The findings in immunocytochemical stained samples and scanning electron microscopy revealed that BrdU labeled nuclei were observed in the cochlea in all of the deafened animals most commonly in the regions of the first-row and second-row Deiter's cells (DCs) and occasionally in the regions of the third-ruw DCs.Conclusion We suggest that, under sufficient drug and enough time, the bat cochlear supporting cells can directly transdifferentiate into the outer hair cells after aminoglycoside exposure. This transdifferentation process is essential for repair of outer hair cells and recovery of normal function after gentamicin exposure.