Ⅰ型胶原蛋白基因在反流性食管炎、Barrett食管及食管腺癌中的表达

目的探讨反流性食管炎、Barrett食管及食管腺癌黏膜组织中Ⅰ型胶原蛋白(COL1A1)基因mRNA的表达水平及意义。方法经胃镜取材,采用荧光定量PCR方法检测反流性食管炎、Barrett食管、食管腺癌组织中COL1A1基因mRNA的表达水平。结果 COL1A1mRNA在正常组和反流性食管炎阴性组食管黏膜中呈低表达(0.154±0.119,0.152±0.105),在Barrett食管与食管腺癌食管黏膜中呈明显的高表达(0.396±0.170,0.726±0.561)。并且在从正常→反流性食管炎→Barrett食管→食管腺癌的发生、发展过程中,COL1A1mRNA的表达呈现渐进性增强,并与病期有关。结论 COL1A1基因的异常表达与反流性食管炎、Barrett食管和食管腺癌的发生、发展密切相关;COL1A1mRNA的表达水平可用作监测Barrett食管和食管腺癌早期发生及干预治疗效果的有用指标。     

心脏传导阻滞SCN5A基因L1001Q突变体构建和功能研究

目的对作者发现的一个中国3代人心脏传导阻滞家系SCN5A基因新突变L1001Q,构建突变表达载体,利用分子生物学方法和全细胞膜片钳技术观察L1001Q突变的功能。方法一步定点诱变法构建pEGFP-L1001Q-SCN5A突变体;激光共聚焦和Western blotting技术检测突变蛋白定位和表达;全细胞膜片钳技术观察突变体钠电流。结果野生型和L1001Q突变均表达在细胞膜上,且表达量无明显变化。二者最大激活电流分别为(148.34±0.77)pA/pF和(132.59±0.96)pA/pF(P>0.05),L1001Q突变半失活电压V1/2为(-73.25±0.87)mV,较野生型正向转移约3.5mV[V1/2为(-76.71±0.73)mV,P<0.05],L1001Q突变失活后恢复时间常数较野生型稍减小,差异无统计学意义(WT tau=16.8ms,L1001Qtau=14.1ms,P>0.05)。结论 L1001Q突变未影响钠通道蛋白的表达和转运,主要通过改变钠通道门控特性引起钠通道功能减弱进而导致心脏传导阻滞。     

广东平兴高速公路河头互通立交方案设计

介绍了广东平兴高速公路河头互通立交方案设计。本次方案设计根据河头互通立交的地形、交通量,在保证行车安全、舒适的前提下,在不同的互通立交选形、布设方案、不同的位置、不同的交叉方式等方面进行全方位比较,最终灵活采用变异A型单喇叭,充分体现了山区高速互通立交的设计特点。     

帕杰罗V97自动变速器电控系统元件功用和检修

简要介绍新型帕杰罗V97装用的INVECS—ⅡV5A5自动变速器电控系统的工作原理,详细论述电控元件的功用、工作原理和检测方法。     

重汽HOWO和A7空调系统原理分析及故障检测

以中国重汽主要车型——HOWO和A7为例,介绍车载空调系统的基本组成、线路连接,详细阐述空调控制原理和HOWO、A7空调控制的异同点,最后对空调系统的故障检测进行详细的说明。     

奥迪A4转角传感器故障解决办法

分析奥迪A4 1.8T轿车正常行驶时,ABS和ESP报警灯间歇性报警产生的原因;介绍利用VAS5051对转向盘转角传感器进行校零和对ABS控制单元进行编码等两种解决方法,并对故障导航操作过程进行说明。     

基于GSM技术和GPS技术的车用远程跟踪报警器设计

利用GSM通讯技术可以克服现有常规车用声光报警器报警距离短、报警形式单一等不足。GPS技术的采用可以提供车辆的地理坐标。设计了以SPCE061A为核心的车用远程跟踪报警器,论述了硬件的工作原理,并给出了相应的软件流程图。样机的试用效果良好、可靠性高,可推广应用到多种需要进行位置监测的场合。     

Preliminary study on Salvia miltiorrhiza bung endophytic fungus

Objective To select the strains which can produce tanshinone ⅡA like its host plant Salvia miltiorrhiza bung. Methods A total of 50 strains of endophytic fungi were isolated from healthy, living and symptomless tissues of Salvia miltiorrhiza bung, among which 29 strains were obtained from the root, 14 from the stem, 3 from the leaf, 3 from the flower and 1 from the seed. Their antimicrobial activities against nine different bacteria, including both Gram-negative and Gram-positive bacteria, were measured by Oxford plate agar diffusion bioassay. Results Our data showed that all but four strains had significant antibacterial activities on at least one indicator bacterium to some extent, and five strains (DR1, DR4, DR16, DR18 and DF2) manifested quite prominent antibacterial activities against certain pathogenic bacteria. In some degree, it might indicate that this endophytic fungus isolated from the tissues of Salvia miltiorrhiza bung has a potential value as a natural antibacterial medicine as well. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were carried out to test selected strains, both inside and outside of the cell to see if any strain can produce tanshinone ⅡA. The result showed that extracts from three strains, labeled as DR12 (outside cell), DR21 (inside cell) and DF3 (inside cell), had a component with the same Rf value in TLC assay as that of authentic tanshinone ⅡA. The extract from DR12 (outside cell) and DR21 (inside cell) had a peak at retention time identical to that of authentic tanshinone ⅡA in HPLC. Conclusion The fungi appear to produce the bioactive compound tanshinone ⅡA, and they could be used to produce tanshinone ⅡA by fermentation. It provides a new way to synthesize this natural medicine.     

列车运行控制仿真系统-轨道电路模块的设计与实现

ZPW-2000A无绝缘轨道电路由较为完备的轨道电路传输安全性技术及参数优化的传输系统构成,为"机车信号做为主体信号"创造了必备的安全基础条件.本文对轨道电路状态和轨道码序仿真为CTCS2级列控系统的实现创造了必要条件.     

预应力度对简支梁桥上拱的影响

对于预应力混凝土(PC)受弯构件,施加预应力将使其产生向上的反拱挠度,预应力度的大小直接影响着预应力混凝土构件上拱值。依托出现明显上拱问题的渝黔高速公路K44平摊子大桥,利用其设计参数,分析部分预应力A类构件和B类构件2种预应力度时所产生的不同上拱度值,得出预应力度对PC简支梁桥上拱的影响。分析表明,预应力度过大将导致梁体产生过大的上拱值。