沥青混凝土路面早期病害成因分析及处治方法

从工程实际出发,对沥青路面早期病害成因进行分析,提出对裂缝处进行压浆处理、在基层松散处设置横向排水沟的措施,针对施工前、施工中的检测控制等提出在实际施工中的有效质量控制措施和注意事项,从而保证施工质量,以达到预防性养护的目的。     

简支T梁病害分析及加固技术

对简支T梁桥普遍存在病害进行总结和成因分析,阐述几种常见的实用性加固技术,介绍各自特点和加固原理,说明各加固方法可以有效地提高简支T梁承载能力。对我国现阶段T梁桥加固具有重要研究价值和指导意义。     

高速公路沥青路面质量通病及防治措施

沥青路面在高速公路中占有很大比例,路面质量直接关系到行车安全。结合工程实践,分析沥青路面中常见病害的产生原因及表现形式,针对病害提出防治措施。     

基于图像处理的高速公路路面损坏检测技术研究

通过介绍基于图像处理的数据采集设备、检测系统和数据处理方法,对图像处理的高速公路路面损坏检测技术进行深入研究,可为道路养护提供参考。     

运三高速公路路面病害调查及处治措施

通过对路面进行钻芯取样、车辙、强度、破损状况、平整度、线形、交通事故等调查,摸清了运三高速公路路面病害严重程度和分布情况,以及沿线交通安全设施的不足之处。根据调查结果,分别就路面工程、排水工程、交通安全设施等提出了相应的处治措施。通过处治方案的实施,认为方案合理、可行,可为同行提供参考。     

Graves病患者甲亢性肝损害及其相关因素分析

目的 探讨Graves病(GD)患者甲亢性肝损害的发生率及其相关因素.方法 GD患者280例,分为未治组(131例)和经治组(149例),检测肝功能指标、血清甲状腺激素水平、甲状腺自身抗体.对其肝功能损害的发生率、临床特点及相关因素进行分析.结果 在未治组甲亢性肝功能损害的发生率为70.99%,经治组为61.07%;甲亢性肝功能损害最常见的异常指标是碱性磷酸酶(ALP)增高,未治组、经治组分别为57.3%、51.0%;血清谷丙转氨酶(ALT)异常在两组的发生率分别为28.2%、21.5%;血清谷草转氨酶(AST)异常在两组的发生率分别为18.3%、15.4%;总胆红素异常在两组的发生率分别为21.4%、20.1%;谷酰胺转肽酶(GGT)异常的发生率未治组和经治组分别为10.7%、4.7%;甲亢性肝功能损害的发生与病程、甲状腺疾病家族史、性别、TGAb水平、TMAb水平无关,与年龄、甲状腺激素水平有关.结论 GD患者甲亢性肝损害较常见,肝功能损害最常见的异常指标是ALP、ALT,甲亢性肝功能损害与甲状腺激素水平、年龄有关.     

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.     

降低沥青路面温度的热反射涂层性能研究

采用一种新型路面热反射涂层涂布于沥青路面表面,在夏季高温季节时,可有效降低路面温度5~10℃,将该涂料使用在排水性大孔隙路面中降温效果更好;同时对该涂料进行了综合路用性能分析,研究表明,热反射涂层铺装具有良好的耐磨性;随着涂层用量的增加,涂层的抗滑性降低,在密级配试件中使用该涂层时必须添加防滑粒料。由于涂层的高反射率可降低路面温度,因此该涂层对路面车辙病害的预防及缓解具有良好的作用。     

桥梁板式橡胶支座病害分析及对策

支座是桥梁结构的一种特殊而重要的部件,板式橡胶支座广泛应用于各种形式的桥梁结构中。该文对板式橡胶支座的常见病害及原因进行了分析,并从施工过程和日常维护方面提出了相应的对策措施。     

广州市珠江东、西公铁两用桥公路桥维修加固

该文针对广州市珠江东、西公铁两用桥既有的病害状况,分析其病害原因,介绍其维修加固方案设计、关键施工工艺。