5羟色胺受体可塑性在大鼠肠易激综合征中的作用

目的探讨5羟色胺(5-HT)以及5-HT3、5-HT4受体可塑性在肠易激综合征(IBS)不同亚型发病中的作用。方法成年雄性SD大鼠27只,随机分为IBS合并腹泻组(IBS-D)I、BS合并便秘组(IBS-C)和空白对照组。分别采用乙酸灌肠、冰水灌胃的方法制备IBS-D和IBS-C大鼠模型。用5-HT以及5-HT3、5-HT4受体抗体的免疫组化方法检测各组大鼠肌间神经丛的5-HT及其受体数量的变化。结果①大鼠肌间神经丛5-HT阳性肠神经元数目IBS-D组(4.37±0.88)显著高于对照组(2.99±0.41,P<0.01),而IBS-C组(3.04±0.35)和对照组相比无差异(P>0.05);②大鼠肌间神经丛5-HT3受体免疫组化染色的灰度值各组间差异无统计学意义(F=0.082,P=0.922);③大鼠肌间神经丛5-TH4受体免疫组化染色的灰度值IBS-D组(141.98±6.96)显著低于对照组(159.90±2.55,P<0.01),而IBS-C组(155.62±2.03)与对照组差异无统计学意义(P>0.05)。结论大鼠肠肌间神经元内的5-HT含量及5-HT4受体数量的显著增多可能与IBS-D大鼠的发病相关。     

Toxicity to the normal hemocytes by ALA-PDT for the ex vivo purging of hematopoietic stem cell grafts

Objective To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control;Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results After ALA-PDT treatment the survival rate of PBMCs had no significant change;however in PBSCs and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA,except for PBMCs, intraceUuiar PplX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosts. Conclusion Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologons bone marrow or stem cell grafts.     

5000 DWT油船改装为硫磺船的改装工艺

本文主要从船体的角度出发,阐述5000DWT油船改装为硫磺船的改装工艺、难点、安全管理、质量管理、周期控制等。     

直线电机牵引方式在城轨交通选线中的优势

以广州市轨道交通5号线为例,通过对直线电机和常规轮轨两种运载系统在选线中的对比,特别在城市中心建筑物密集、地上地下建构筑物复杂、地质复杂和人口密集地区中的选线对比,阐述直线电机的优势所在,使一些采用常规轮轨系统无法实现站点的设置成为可能,同时展示直线电机运载系统在国内城市轨道交通中的应用前景。     

基于新基建背景下汽车专业教学改革

基于"新基建"的背景,论述新基建与汽车行业的紧密联系,提出高职院校汽车专教学改革,以培养具有数字技术的专业人员为目标,以5G技术与大数据为中心进行培养模式构建,为我国高职院校汽车专业人才培养提供一定的理论基础和实际指导意义.     

超薄磨耗层SMA-5沥青混合料的设计及性能研究

采用改进的主骨料空隙体积填充法进行了超薄磨耗层SMA-5沥青混合料的配合比设计,经室内试验验证SMA-5沥青混合料路面具有良好的路用性能.试验路检测结果表明,与密级配沥青混凝土路面相比,超薄磨耗层SMA-5沥青路面的表面构造深度提高38%,摩擦系数提高14%,路面噪音降低3～4 dB,具有构造深度大、抗滑性能好、降噪效果明显的特点.     

跨平台船用JLR-7700MK2型GPS仿真系统

由于大部分航海院校及培训机构采用真机设备进行船用GPS设备的教学培训,而采用真机培训成本较高,受场地因素制约,偏离航向和动态航行信息显示等功能无法在实验室环境内完成。基于大圆航线算法实现了仿真设备的航线设计和绘制功能,仿真结果更符合航海实践。采用CSS技术对GPS仿真系统界面进行布局,实现了仿真界面的自动适配功能。基于HTML5技术进行系统的跨平台开发,实现了PC端和移动端JLR-7700MK2型GPS设备的仿真。该系统实现了GPS真机的主要功能的基础上,有效的弥补了真机受场地约束的缺陷,并能够跨平台使用,可满足航海院校及培训机构学生的实操培训需求。     

The potential health,financial and environmental impacts of dieselgate in Ireland

ABSTRACT

The transportation sector is the greatest contributor to air pollution. With the booming demand for transportation, reducing the pollution has become one of the main concerns of researchers. EPA emission standards are designed to protect air quality and human health. Diesel Euro 5 NO_x has become a matter of disquiet since it has been found that NO_x emissions are significantly exceeding the standard limit. This paper presents a study to estimate the disparity in real-world NO_x emission levels resulted from all diesel Euro 5 passenger cars (PC) and light commercial vehicles (LCV) that are present in Ireland. NO_x emission levels calculated based on laboratory test results, on-road measurements and the COPERT 4 model were compared. Additionally, NO_x emission levels from the defective Volkswagen models have been calculated to quantify the effect of the Volkswagen scandal on Ireland. Impacts of excess NO_x emissions on health and cost have also been presented.     

CATIAV5在现代整车开发中的应用

CATIAV5可为数字化企业建立一个针对产品整个开发过程的工作环境,在现代汽车整车开发中具有重要作用。文章介绍了目前最新的CATIAV5软件,从A级曲面造型设计、结构工程设计到DMU装配分析、CAE仿真分析,多角度全方位地阐述了当前汽车开发的各个阶段及CATIAV5软件在各个阶段中所起的作用。指出CATIAV5作为汽车设计的核心工具．其可伸缩性和并行工程能力可显著缩短产品上市时间,已被各国的汽车零部件供应商认可。     

ANPC五电平逆变器SVPWM与SPWM的统一理论研究

目前多自由度多电平领域的空间矢量调制(SVPWM)和三角载波正弦脉宽调制(SPWM)的统一理论研究较少.本文在线电压坐标系下,对有源中点钳位型五电平逆变器(5L-ANPC)的SVPWM调制技术进行分析,研究开关序列L和复用矢量调节系数k对SVPWM等效调制波的影响.用SPWM调制波加零序电压的方式等效SVPWM,并推导...