Fas、FasL在非小细胞肺癌中的表达及其与肿瘤免疫和细胞凋亡的关系

目的为探讨Fas/FasL系统在非小细胞肺癌(NSCLC)发生发展中所起的免疫逃逸作用,为临床早期诊断肺癌、判断预后、设计新的治疗策略提供依据。方法采用免疫组织化学方法检测Fas、FasL在NSCLC组织及正常肺组织中的表达,同时采用抗CD45RO抗体检测肿瘤组织中浸润淋巴细胞(TIL)的数量,应用TUNEL技术检测肺癌细胞凋亡。结果 Fas在肺癌组织低表达并存在异位表达,表达率与细胞分化有关;FasL在肺癌组织中高表达,表达率和细胞类型及淋巴结转移相关,与TIL浸润程度呈负相关,Fas表达与肺癌细胞凋亡水平无关。结论 NSCLC肺癌组织中普遍存在Fas表达的下调、异位表达及FasL高表达,导致癌细胞的免疫逃逸,参与NSCLC的发生和发展。     

谈高职院校图书馆的读者咨询服务

本文从高职院校图书馆工作性质出发,提出读者咨询活动在图书馆工作中的重要作用。再通过分析高职院校图书馆咨询服务存在的问题,从而提出怎样才能更好地完成高职院校图书馆的读者咨询活动。     

Analysis and determination of diterpenoids in unprocessed and processed Euphorbia lathyris seeds by HPLC-ESI-MS

Euphorbia lathyris （Caper spurge） is a toxic and potent Chinese materia medica （T/PCMM）. This study sought a method for identifying five diterpenoids （Euphorbia factors LI-L3, L7a, and Ls） with the spectra of UV and mass, quantifying three diterpenoids L1, L2, and L8 in crude extracts of unprocessed and processed E. lathyris seeds by liquid chromatography/ electrospray ionization mass spectrometry （LC-ESI-MS）. The analysis was achieved on an Agilent Eclipse XDB-C18 column （4.6 mm× 150mm i.d., 5 μm） with an isocratic elution with a mobile phase consisting of water and acetonitrile at a flow rate of 0.25 mL/min at column temperature of 30 ℃ and UV detection was set at 272 nm. An ESI source was used with a positive ionization mode. The calibration curve was linear in the ranges of 9.9-79 μg/mL for Euphorbia factor Lb 3.8-30.5μg/mL for Euphorbia factor L2, and 1.0-20.6 μg/mL for Euphorbia factor LB. The average recoveries （n=6） of three diterpenoids were 98.39%, 91.10% and 96.94%, respectively, with RSD of 2.5%, 2.4% and 2.1%, respectively. The contents of the three diterpenoids in processed E. lathyris seeds were 3.435, 1.367 and 0.286 mg/g, respectively, which decreased more sharply than those in unprocessed E. lathyris seeds which were 4.915, 1.944 and 0.425 mg/g, respectively. The method is simple, accurate, reliable and reproducible, and it can be applied to control the quality of unprocessed and processed E. lathyris seeds.     

“STCW78/10修正案”对我国航海英语教育的影响及其对策

文章针对"STCW 78/10修正案"对海员有效沟通和交际能力提出的新标准,剖析了新标准对我国航海英语教育的影响,在对比分析新旧标准的基础上,探讨了新标准下航海英语教育的对策,以提高我国航海英语教育整体质量。     

基于模糊控制的车辆侧倾稳定性联合仿真

利用ADAMS/Car建立起车辆虚拟样机模型,通过试验验证了模型的正确性;在模糊控制方法的基础上,设计出横摆角速度反馈控制器和质心侧偏角反馈模糊控制器,以及两者联合反馈控制的模糊控制器;最后通过ADAMS/Car与MATLAB/Simulink联合仿真对这3种控制器进行研究.为车辆侧倾稳定性控制提供了一种有效的方法.     

Changing accessibility, dwelling price and the spatial distribution of socio-economic activities

In this paper we present an activities-location choice model with endogenous price which simulates, based on Expected Random Utility principle, the behaviour of several agents of the urban system (e.g. the workers distinguished by income, the firms by economic sector) to estimate the spatial distribution of socio-economic activities within the study area as well as the impact of differential changes in accessibility on the dwelling price. The study area for this research is the metropolitan area of Napoli (South Italy), for which we show the results of the model estimation and the results of a “backcasting” analysis.     

LNG船液货装卸系统分析软件平台研究

提出了一种基于VC＋＋平台和数据库技术的LNG船液货装卸系统设计及分析的软件平台系统,详细介绍了其内容、结构和实现方法。该系统配有与管路阻力计算相关的管道、阀件、液货舱、附件等的数据库,能够按目标船的装卸要求,直接组态设计液货装卸系统管网系统。设计人员可以在每一个模型设计完成后直接进行优化并确定液货泵的排量、压头、液货管路的管径、长度等设计信息,仿真计算装卸过程中各时间段上的的流量、节点压力、剩余舱内液体容积等各项参数。不仅简化了液货装卸系统的设计过程和计算校核的时间,而且更加方便灵活。     

双向DC-DC变换器软开关技术研究

本文分析了移相全桥ZVS变换技术的工作原理,给出了谐振电感的选取条件,采用双向DC-DC变换的移相控制策略对设计电路进行了仿真,仿真结果验证了理论分析的正确性.     

半潜船总体性能设计

论述了我国首艘拥有自主知识产权、多个技术指标达到或超过国际水平、自主研发的自航半潜船"WISHWAY"的船型特点及主要总体性能设计。     

三电平SVPWM变频调速系统的PSCAD/EMTDC仿真

本文分析了三电平空间矢量脉宽调制的基本原理,应用电磁暂态分析软件PSCAD/EMTDC搭建了基于三电平逆变器的感应电机调速系统模型,实现了电机的矢量控制。仿真结果证明了该方法的有效性。