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61.
目的观察大鼠肢体缺血再灌注(LIR)后骨骼肌细胞凋亡情况,探讨内皮素A(ETA)受体拮抗剂BQ123对其的保护机制。方法雄性Wistar大鼠随机分为3组(n=8):对照组、肢体缺血再灌注组和选择性ETA受体阻断剂BQ123处理组,缺血4 h再灌注4 h后检测血浆一氧化氮(NO)、内皮素(ET-1)、血栓素B2(TXB2)、前列环素(6-Keto-PGF1α)的含量以及NO/ET-1比值、TXB2/6-Keto-PGF1α比值的变化;采用免疫组织化学方法检测腓肠肌组织中凋亡相关基因Bcl-2、Bax和凋亡蛋白酶(Caspase-3)的蛋白表达情况,利用原位脱氧核糖核甘酸末端转移酶介导的缺口末端标记法(TUNEL)检测各组腓肠肌细胞的凋亡情况。结果再灌注组与对照组相比较,血浆NO、ET-1、TXB2、6-Keto-PGF1α含量均增加,但TXB2/6-Keto-PGF1α比值增大,NO/ET-1比值减小,凋亡指数(AI)明显增高,Bax、Bcl-2、Caspase-3蛋白表达明显较对照组增强,但Bcl-2/Bax比值减小;与再灌注组比较,BQ123处理组血浆NO、6-Keto-PGF1α含量升高,ET-1、TXB2下降,NO/ET-1比值升高,TXB2/6-Keto-PGF1α比值下降;腓肠肌组织Bcl-2/Bax比值升高,Caspase-3表达水平明显减弱,AI下降。结论 ETA受体拮抗剂BQ123可减轻大鼠肢体缺血再灌注后骨骼肌细胞凋亡。 相似文献
62.
夏长凤 《南通航运职业技术学院学报》2011,(4):117-120
文章以南通航运职业技术学院机电系电气自动化专业(楼宇方向)的课程体系改革为例,深入分析了"3+2+1"教学模式的内涵,指出该专业课程体系的构建将传统教学的优势与以项目为主体、以工作过程为导向的教学方式有机地结合,实现了模块化教学,激发了教师教学和学生学习的积极性。 相似文献
63.
侏秀华 《大连铁道学院学报》2000,21(1):82-84
研究了烃类化合物在OV-1和SE-54固定相间的焓变关系,该研究结果为设计烃类化合物在相同温度程序下的双柱谱图自动识别程序提供了科学依据。 相似文献
64.
文章针对高职院校三年制教学模式下公路运输与管理人才培养的弊端,结合公路运输行业的发展实际,提出公路运输与管理专业推行“2+1”实训教学模式改革的必要性,阐述了分阶段、循环递进式的实训教学模式及实施流程,并评述了该教学模式取得的成效与存在的不足. 相似文献
65.
当前时期,针对复合型人才教学体系的创新,应当有对性地构建起更加完善的人才培养体系,并做好对于学生的专业性知识技能和专业性职业技能优化培养。结合现阶段的新能源汽车技术专业培养,要针对把握性教学标准和教学要求,做好有效性的更新和完善,本文主要结合基于"1+3"的复合型人才课程体系的探索与实践展开了研究分析。 相似文献
66.
The Arabian Sea exhibits a complex pattern of biogeochemical and ecological dynamics, which vary both seasonally and spatially. These dynamics have been studied using a one-dimensional vertical hydrodynamic model coupled to a complex ecosystem model, simulating the annual cycle at three contrasting stations. These stations are characterised by seasonally upwelling, mixed-layer-deepening and a-seasonal oligotrophic conditions, respectively, and coincide with extensively measured stations on the two JGOFS ARABESQUE cruises in 1994. The model reproduces many spatial and temporal trends in production, biomass, physical and chemical properties, both qualitatively and quantitatively and so gives insight into the main mechanisms responsible for the biogeochemical and ecological complexity. Monsoonal systems are typified by classical food web dynamics, whilst intermonsoonal and oligotrophic systems are dominated by the microbial loop. The ecosystem model (ERSEM), developed for temperate regions, is found to be applicable to the Arabian Sea system with little reparameterisation. Differences in in-situ physical forcing are sufficient to recreate contrasting eutrophic and oligotrophic systems, although the lack of lateral terms are probably the greatest source of error in the model. Physics, nutrients, light and grazing are all shown to play a role in controlling production and community structure. Small-celled phytoplanktons are predicted to be dominant and sub-surface chlorophyll maxima are robust centers of production during intermonsoon periods. Analysis of carbon fluxes indicate that physically driven outgassing of CO2 predominates in monsoonal upwelling systems but ecological activity may significantly moderate CO2 outgassing in the Arabian Sea interior. 相似文献
67.
铁路旅客周转量的改进等维新息模型预测 总被引:1,自引:1,他引:0
铁路旅客周转量预测不仅影响铁路客运计划组织工作,它还体现了客运市场中铁路运输的市场控制力。针对灰色模型对数据的要求,采用滑动平均处理和对数化处理原始数据,并采用等维新息模型对铁路旅客周转量进行预测,通过与实际情况和GM(1,1)模型的预测效果的对比可以发现,该模型可以通过检验并且预测效果良好。 相似文献
68.
69.
本设计提出了一种基于CPLD的0.01S计时器的设计方案。应用高速集成电路以硬件描述语言VHDL语言描述,采用Altera公司的可编程逻辑器件(CPLD)EPM7064SL84实现的现代智能计时仪表,为广大用户提供了方便。系统具有可靠性高,便于功能扩展,成本低,易普及等优点。 相似文献
70.
Using CalCOFI data for coastal shallow stations (above 100 m depth), higher than expected nitrate concentrations were detected in near-surface high-temperature waters off of Central Baja California during some El Niño winters. Though recent data are not available for Central Baja California, past El Niño data, though limited, showed nitrate concentrations above 16 μM at temperatures above 16 °C, and nitrate concentrations between 1 and 2 μM at 19 °C, while the previously established relationship of temperature and nitrate for California Current waters predicts nitrate depletion above 14 or 15 °C. The anomalous, high temperature–high nitrate enrichment events documented in Central Baja California were detected as shallow as 9 m and as deep as 73 m, were associated with low-oxygen (between 2 and 4 ml/l) and high-salinity (between 33.8 and 34.3 psu) waters, and occurred during the winter months of an El Niño year. Using recent data for San Diego, CA, similar but weaker enrichment events were detected for the El Niño winter of 1997–1998. The periodic shoaling of a subsurface subtropical water mass of high temperature, high salinity, low oxygen and high nutrients during some El Niño winters is proposed to cause periodic enrichment and to maintain productivity during warming events in this area. Enrichment events were not detected off Ensenada, in Northern Baja California, possibly due to the amplification of the onshore flow during El Niño there, or due to the Ensenada front. The proposed mechanism of periodic enrichment of nutrient-depleted surface waters during some El Niño winters by subsurface waters from the California Undercurrent may explain the following: (1) survival of giant kelp forests at their southern limit in Central Baja California documented during past El Niño events in warm waters, (2) the rapid recovery and high carrying capacity of giant kelp documented after the mass disappearance during El Niño 1997–1998, and (3) the increase in the extent of mesotrophic chlorophyll detected in the area during the 1997–1998 and 1982–1983 El Niño events. 相似文献