全反式维甲酸对肾间质纤维化大鼠肾间质巨噬细胞浸润的影响

目的探讨全反式维甲酸(all-trans-retinoic acid,at-RA)对肾间质纤维化大鼠肾间质巨噬细胞浸润的影响。方法采用单侧输尿管结扎(unilateral ureteral obstructive,UUO)大鼠模型。40只大鼠随机分为假手术组、模型组、苯那普利组、维甲酸小剂量组、维甲酸大剂量组。于造模前1 d至造模后14 d分别给予维甲酸小剂量组、维甲酸大剂量组、苯那普利组全反式维甲酸10 mg/(kg.d)2、0 mg/(kg.d)和苯那普利10 mg/(kg.d)。假手术组、模型组给予等量的生理盐水。用免疫组化染色观察肾间质巨噬细胞数和Ⅲ型胶原(collagenⅢ,ColⅢ)的表达。并常规行HE和Masson染色观察肾小管间质损伤指数。结果维甲酸和苯那普利均能显著抑制肾间质巨噬细胞的浸润(P<0.01),并且减低肾小管间质损伤指数和ColⅢ的表达(P<0.05)。结论维甲酸及苯那普利可能通过抑制肾间质巨噬细胞浸润而减轻UUO大鼠的肾损害。     

“双骨胶囊”治疗大鼠实验性骨质疏松的效果观察

目的　观察“双骨胶囊”治疗骨质疏松的效果。方法　 5～ 6月龄SD雌性大鼠 ,以维甲酸诱导骨质疏松模型后 ,给予含硼中药“双骨胶囊”进行治疗。用骨病理组织学、形态计量学等实验技术 ,观察“双骨胶囊”的治疗效果。结果　“双骨胶囊”治疗后 ,大鼠的平均骨小梁数 (6 1 0±0 88)、平均骨小梁宽 (75 74± 1 8 3 9)、骨小梁面积百分比 (2 4 0 1± 6 1 4)等指标增加 ,明显高于骨质疏松组 (4 3 3± 0 82 ,41 96± 1 1 6 9,1 6 1 6± 3 0 3 ;P <0 0 5 )。结论　“双骨胶囊”可增加骨量 ,对维甲酸诱导的大鼠骨质疏松有治疗作用     

绞股蓝总皂甙对体外培养肝癌细胞核酸和蛋白质合成的影响

作者研究了绞股蓝总皂甙(GP)对人体肝癌SMMC—7721细胞的作用。结果证明,GP具有抑制肝癌细胞的生长及DNA、RNA和蛋白质合成,并观察到加GP组癌细胞中DNA、RNA含量明显减少。     

硒对大鼠肝核糖体含量及其聚集程度的影响

观察了低硒组和加硒组大鼠肝去线粒体上清液（ＰＭＳ）中总核糖体含量、多聚核糖体／总核糖体比值的变化。同时还测定了肝匀浆中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性、核酸含量及ＴＢＡ值。结果表明：低硒组大鼠肝ＰＭＳ中总核糖体含量及其聚集程度均显著低于加硒组，肝ＧＳＨ－Ｐｘ活性和ＲＮＡ含量也明显低于加硒组，而肝ＴＢＡ值升高。     

Cooperation of Science and Management for Harmful Algal Blooms: Domoic Acid and the Washington Coast Razor Clam Fishery

Harmful algal blooms (HABs) may be increasing in frequency and intensity worldwide. Coastal economies suffer significant income losses when fisheries or beaches are closed to protect human health and subsistence fishing communities are at risk. Despite these hardships, managers must often conservatively close harvests across a wide area or for long periods, because they lack scientific information that would allow them to predict HAB events. The outer coast of Washington State has experienced several closures of the razor clam (Siliqua patula) fishery starting in 1991, due to domoic acid (DA) contamination caused by toxic blooms of the diatom Pseudo-nitzschia. Improved science-based management was needed to minimize the impact of DA on this fishery and the coastal communities that relied on it for income, tourism, and subsistence. The Olympic Region Harmful Algal Bloom (ORHAB) Partnership, comprised of state and tribal managers, scientists, and local stakeholders, evolved in response to this need; it has been successful in its mission. Here we examine ORHAB through the lens of the Institutional Analysis and Development framework, in order to identify key factors contributing to its success. The relevance of our findings for other ORHAB-like institutions in the Pacific Northwest and elsewhere is discussed.     

新型斜拉索冷铸锚灌注料试验研究

通过新型液态酸酐灌注料试验,测试新型酸酐灌注料的性能,验证其完全满足斜拉索冷铸锚灌注料的性能要求,且韧性更好,可用于斜拉索冷铸锚的灌注.     

基于酸腐蚀的板式氯丁橡胶支座受压试验研究

公路桥梁板式橡胶支座比其他橡胶支座更容易受到酸雨的影响,为了研究不同腐蚀时间下公路桥梁板式氯丁橡胶支座经过酸腐蚀后的各项力学性能变化,采用3．0％的硫酸溶液对公路桥梁氯丁橡胶支座分别进行20,40,60和80 d的酸腐蚀处理,并采用5000 kN压力试验机进行轴心受压试验研究．分别对其表观、外形尺寸、极限抗压强度、竖向刚度和抗压弹性模量等进行对比分析．结果表明腐蚀时间越长,公路桥梁板式氯丁橡胶支座更容易发生脆性破坏,其极限抗压强度、竖向刚度和抗压弹性模量等指标显著降低．通过承载力衰减规律对比分析,采用最小二乘法建立公路桥梁板式氯丁橡胶支座的极限抗压强度的衰减模型．当腐蚀到一定程度,极限抗压强度、竖向刚度和抗压弹性模量等已无法满足实际工程要求,建议在实际工程中要避免氯丁橡胶支座受到酸腐蚀的破坏．     

CHANGES OF ANTIBODIES AGAINST ANTIGENS ENCODED BY DIFFERENT REGIONS OF HCV GENOME IN CHRONIC HEPATITIS C PATIENTS TREATED WITH INTERFERON

Interferon(IFN)isthefirstchoicefortreatingchronichepatitisC(CHC),how-ever,onlylO%～25%ofthepatientsshowsustainedresponsetoIFN.ThereisnoreliablemarkertoevaluatetheefficacyofIFNtreatment.Theopinionsonthesig-nificanceofthechangesofantibodiestoantigensencodedbydifferentregionsofHCVgenomeinpatientstreatedwithIFNarecontroversial.Wedevelopedarecom-binantimmunoblotassay(RIBA)includ-ingthreerecombinantantigensofHCV(C22,C33c,Cloo-3),andfollowedup2OchronichepatitisCpatientstreatedwithIFN,to…     

维甲酸胶囊的制备及临床应用

研究了治疗骨髓增生异常综合症（ＭＤＳ）的药物维甲酸胶囊的处方、生产工艺及质量标准。应用维甲酸胶囊治疗４例骨髓增生异常综合症，剂量为６０～１２０ｍｇ／ｄ不等，１例２３ｄ共服用２７６０ｍｇ，全部获得好转。４例患者在用药期间均未出现肝功能损害。     

植物纤维增强水泥管耐强酸强碱腐蚀研究

市政排水管道常用水泥管或钢筋混凝土管作为主要管材,市政污水可能会对管道造成腐蚀。研究了植物纤维增强水泥管在pH值为1、2、3、14的溶液中浸泡一个月的腐蚀情况,通过表观观测、质量和抗压强度测定,以耐蚀系数和质量损失率两个指标判定植物纤维增强水泥管在试验条件下的腐蚀情况。结果表明,植物纤维增强水泥管在强酸强碱溶液中浸泡一个月,质量和抗压强度均无明显损失,其耐久性有一定的保障,可以用作市政排水管道建设。