地铁工程地质剖面图典型图例符号自动识别研究

地铁工程地质剖面图中包含施工安全风险辨识所需的重要信息,地质剖面图的自动识别是风险识别自动化系统的重要环节。论述了地铁工程地质剖面图图例的分类,图例识别面临的困难与识别可行性。以"黏土"和"粉质黏土"图例识别为例,描述了识别方法和详细算法。以武汉地铁2号线某车站围护结构地层信息识别为例,示范了图例识别的具体应用。     

反坡斜井突涌水施工技术

通过对锦屏山隧道辅引3#施工支洞反坡施工,介绍反坡斜井突涌水条件下施工技术,根据实例论述了以防为主、防排结合的反坡斜井突涌水隧道施工方法.     

堆场和道路智能照明的节能与推广

阐述高压钠灯功率、亮度和寿命与电压的关系,分析能对电网电压波动快速响应,闭环自动稳压、可控平缓调压的智能装置的工作原理和节能模式,应用它在软启动和运行时,灯具电流几乎恒等于额定电流,因而,不仅可以大量节约电能,还可以延长灯具的使用寿命,并探讨它在集装箱堆场应用集中远程控制的方案。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

镀锌钢带恒速卷绕的计算机控制

提出了采用计算机控制实现镀锌钢带以恒定速度卷绕的技术方法.要达到整个卷绕过程中绕线速度恒定,关键是以正确的算法计算,逐字减少D/A转换控制字的时间间隔.     

图像边界检测区域对比度模糊增强算法在轮廓提取中的运用

分析了图像边界检测的区域对比度模糊增强算法的特点，通过实验验证了该算法的正确性和可行性，并指出了算法的一些不足之处和待改进的地方，提出了一种适用轮廓提取的新广义算子。     

汽车维修中检测诊断技术的应用探讨

现如今汽车已经成为人们必不可少的代步工具,汽车的数量随之也越来越多,在汽车的行驶过程中,难免会出现故障之类的问题,因此汽车的维修工作也变的尤为重要,其维修的师傅必须具备丰富的工作经验以及理论知识,然后借助检测诊断技术来进行汽车的事故和问题故障的检测排查工作。本文围绕汽车的检测诊断技术以及该技术在汽车维修中的应用展开分析。     

一种新型城市道路雨水收集与利用系统的研究与设计

结合工程实际,介绍了集汇水、过滤、灌溉、储水和自动排水功能为一体的道路雨水资源收集与利用系统的设计,有关经验可供相关专业人员参考。     

Efficient multiple model particle filtering for joint traffic state estimation and incident detection

This article proposes an efficient multiple model particle filter (EMMPF) to solve the problems of traffic state estimation and incident detection, which requires significantly less computation time compared to existing multiple model nonlinear filters. To incorporate the on ramps and off ramps on the highway, junction solvers for a traffic flow model with incident dynamics are developed. The effectiveness of the proposed EMMPF is assessed using a benchmark hybrid state estimation problem, and using synthetic traffic data generated by a micro-simulation software. Then, the traffic estimation framework is implemented using field data collected on Interstate 880 in California. The results show the EMMPF is capable of estimating the traffic state and detecting incidents and requires an order of magnitude less computation time compared to existing algorithms, especially when the hybrid system has a large number of rare models.     

对船舶污染损害侵权责任及索赔主体问题的探讨

船舶污染事故是造成海洋环境污染损害的主要原因之一,建立完善的船舶污染损害索赔机制,保证受损方获得及时的赔偿,是恢复海洋环境、维护受害者利益所必须的。文中从民法的基本原理出发,对国家海域所有权、海域使用权等进行了论述,并按照民事侵权责任的承担原则,对索赔权利主体的确定等问题进行了探讨。