谈如何搞好公路建设征地拆迁安置工作

通过对公路建设征地拆迁安置工作过程中,业主、设计单位、施工单位、拆迁户等各层面存在或潜在的不稳定、不和谐因素进行分析,有针对性地提出做好公路建设项目征迁安置工作的举措,有助于公路建设征地拆迁安置工作取得良好的社会效益和经济效益.     

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.     

生物膜法在市政水处理中的应用

该文对采用生物膜法进行市政给水污水以及污水厂二级出水的处理进行了综述,表明采用生物膜法水处理技术在市政给排水处理及污水回用领域有着广泛的应用前景,尤其是在对处理微污染水体中的应用前景看好。     

深水铺管船铺管区通风系统设计

以某在建深水(3000 m)铺管船为例,分析各铺管作业区域的工作环境条件.根据工作区域的设备布置和工序操作,设计不同的通风系统气流组织方案并给出具体技术措施,以期改善铺管作业人员的工作环境.研究成果可为类似铺管船通风系统的设计提供一定参考.     

改良 Bardenpho 工艺的设计与运行效果分析

济南某污水处理厂工程设计规模为4万m3/d,二级处理采用改良Bardenpho工艺,同时通过设置多点进水、碳源多点投加,前端设置预缺氧池,增设二级缺氧/好氧可变段等措施,为污水厂提供多种工艺运行模式。运行结果表明:改良Bardenoho工艺对CODCr、NH3-N、TN、TP等污染物去除率分别达到了94.98%、98.35%、93.95%、79.68%,出水CODCr、NH3-N、TN、TP分别为11.7、0.68、3.03、0.77 mg/L,除TP尚需通过深度处理强化去除外,其余指标均优于设计出水水质。     

大型曝气生物滤池污水处理厂提标改造设计

沈阳仙女河污水处理厂是采用曝气生物滤池工艺的大型污水处理厂,处理规模达40万m3/d。在其提标改造工程中,将现状二级曝气生物滤池改造成了带反硝化功能的三级生物滤池,并采用加砂沉淀池作为深度处理单元,使出水水质由《城镇污水处理厂污染物排放标准》（GB 18918—2002）的二级标准提高至一级A标准;同时通过建设应急处理系统,实现了不停产施工、不降低排放标准的预期目标。     

EGR柴油机瞬态性能优化研究

以降低瞬态过程烟度和NO_x排放为目标,在一台高压共轨电控重型柴油机上进行了EGR对柴油机恒转速增扭矩5s典型瞬态过程燃烧和排放性能影响的优化研究。结果表明:瞬态过程中固定EGR阀开度造成EGR率"超调"、烟度剧增;与"全程轨压"策略相比,"分段轨压"有利于改善小负荷工况的燃烧热氛围,提高瞬态起始负荷并耦合"分段轨压"可以有效降低瞬态过程烟度峰值;EGR阀的开闭对瞬态性能影响最大,瞬态过程1.5s关阀、4s开阀的策略可以实现较好的烟度和NO_x排放折中,消光烟度峰值为9.2%,NO_x峰值稍有增加但增幅不大。     

肾衰患者血清泌乳素测定的临床意义

本文对40例肾功能衰竭(简称肾衰)患者血清泌乳素水平与肾衰程度及两者之间的关系进行了对比观察.结果表明:肾衰患者血清泌乳素水平明显高于正常对照组(P<0.0005),肾功能损害越严重,血清泌乳素水平越高(P<0.0005)提示血清泌孔素水平,可反映肾功能损害程度。     

降低城市轨道交通征地拆迁成本的探讨

在大量数据的基础上,分析我国城市轨道交通征地拆迁成本的现状,认为目前这项成本过高且有增长的趋势;从用地控制规划、车辆段规模、资源共享等方面,提出降低征地拆迁成本的措施和方法。     

粉细砂地层地铁盾构端头井加固方法的适用性分析

某地铁的盾构出洞位置处采用了深层搅拌桩进行加固.从竖直和水平方向对成桩质量进行了评估,结果表明质量不合格,不能满足盾构出洞的技术要求.为保证施工安全,又分别利用液氮和盐水冻结对左、右线隧道的端头井地层进行了加固补强.实践表明两种方法都能满足盾构出洞的技术要求.经对比分析认为,液氮和盐水冻结法比搅拌桩更适合应用于粉细砂地...