粉防己碱诱导宫颈癌细胞凋亡的定性定量研究

目的 从定性定量角度探讨粉防己碱诱导宫颈癌HeLa细胞凋亡的作用.方法 采用MTT法检测不同浓度粉防己碱作用不同时间对人宫颈癌HeLa细胞株的增殖抑制作用.通过流式细胞仪和激光共聚焦显微镜检测细胞凋亡情况.结果 粉防己碱对宫颈癌HeLa细胞具有增殖抑制作用,且呈时间、浓度依赖性.应用流式细胞仪检测到15μmol/L粉防己碱诱导HeLa细胞产生的凋亡率为(51.8±0.97)%,显著高于对照组凋亡率(24.3±1.23)%(P<0.05).通过激光共聚焦显微镜观察,与对照组比较,粉防己碱作用后HeLa细胞具有明显的凋亡形态.结论 粉防己碱能够抑制HeLa细胞的增殖并诱导其凋亡.     

国内直肠癌术后吻合口瘘危险因素的Meta分析

目的 探讨国内直肠癌术后吻合口瘘的危险因素.方法 运用 Meta分析方法对我国1999年1月至2009年1月公开发表的有关国内直肠癌术后吻合口瘘危险因素的19篇文献资料进行综合分析.结果 年龄≥60岁的直肠癌患者吻合口瘘的发生率高于<60岁者,合并OR值为0.50(95% CI:0.33～0.76,P<0.01);男性直肠癌患者吻合口瘘的发生率高于女性,合并OR值为2.17(95% CI:1.38～3.42,P<0.01);肿瘤下缘距肛缘距离≤7cm的直肠癌患者吻合口瘘的发生率高于距离>7cm者,合并OR值为1.79(95% CI:1.37～2.35,P<0.01);术前接受新辅助放疗的直肠癌患者吻合口瘘的发生率高于未进行新辅助放疗者,合并OR值为3.66(95% CI:2.19～6.09,P<0.01);吻合器吻合的直肠癌患者吻合口瘘发生率低于手工吻合者,但不具有统计学意义(P>0.05),合并OR值为0.70(95% CI:0.47～1.05);术前患有糖尿病的直肠癌患者吻合口瘘发生率低于无糖尿病者,合并OR值为3.16(95% CI:2.27～4.39,P<0.01);Dukes分期A、B期的直肠癌患者吻合口瘘发生率低于C、D期患者,合并OR值为0.61(95% CI:0.45～0.83,P<0.01);恶性度低的直肠癌患者吻合口瘘发生率低于恶性度高者,合并OR值为2.15(95% CI:1.47～3.15,P<0.01);预防性造瘘的直肠癌患者吻合口瘘发生率低于未进行造瘘者,但不具有统计学意义(P>0.05),合并OR值为0.39(95% CI:0.14～1.05);急诊手术的直肠癌患者吻合口瘘发生率高于择期手术者,合并OR值为0.27(95% CI:0.13～0.56,P<0.01).结论 年龄≥60岁、男性、糖尿病、术前新辅助放疗、肿瘤下缘距肛缘距离<7cm、Dukes分期C、D期、肿瘤恶性程度高及急诊手术是直肠癌术后吻合口瘘发生的危险因素.     

THE SIGNIFICANCE OF PRE- AND POSTOPERATIVE MEASUREMENT OF SERUM CA242 LEVELS IN PANCREATIC CANCER

THE SIGNIFICANCE OF PRE- AND POSTOPERATIVE MEASUREMENT OF SERUM CA242 LEVELS IN PANCREATIC CANCER@陈熹@秦兆寅@纪宗正@黎一鸣l Hag1und C, Lindgren J, Roberts P et al. Tis-sue expression of the tumorassociated antigenCA242 in benign and malignant pancreatic le-sions: a comparison with CA5O and CAl9-9.Br J Cancer, l9891 6O(5): 845 2 Kuusela P, Haglund C, Roberts P. Compari-son of a new tumor marker CA242 with CAl9-9, CA5o in degestive trac[ diseases. B…     

原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系

目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA,通过RT-PCR扩增Fra-1全长基因,将其克隆入pcDNA3.1(-)myc-his表达载体;应用脂质体法转染MCF-7细胞,观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移,在肿瘤细胞的侵袭和转移过程中发挥作用。     

CD44v6和E-cadherin在肺癌中表达及其临床意义

目的　为探讨白细胞分化抗原变异型 (CD44v6 )和上皮型钙粘蛋白 (E cadherin ,E cd)基因在评估肺癌生物学行为及预后中的作用。方法　采用免疫组织化学SP法对 46例肺癌和 1 5例癌旁组织中CD44v6和E cd的表达进行研究。结果　CD44v6主要表达于非小细胞肺癌(NSCLC) ,与其临床分期、淋巴结转移呈正相关 (P <0 0 1、0 0 5 ) ;E cd在肺癌组织中的阳性表达率显著低于癌旁组织 (P <0 0 1 ) ,且与肺癌TNM分期、病理分级及淋巴结转移呈负相关 (P <0 0 5、0 0 1、0 0 5 ) ;CD44v6和E cd在NSCLC中表达显著相关 (P <0 0 5 )。结论　CD44v6和E cd基因对评估肺癌进展及判断预后具有一定价值。     

Cyclin E及p53的表达与早期胃癌进展及淋巴结转移的关系

目的　了解CyclinE及 p5 3的表达与早期胃癌进展及淋巴结转移的关系。方法　用免疫组织化学方法检测了 1 0 8例早期胃癌CyclinE及p5 3的表达。结果　CyclinE及p5 3的表达与病人性别及肿瘤组织学类型有关 (P <0 0 5 )。CyclinE异常表达与肿瘤部位、浸润深度及胃壁淋巴管浸润有关 (P <0 0 5 )。CyclinE及p5 3联合异常表达的肿瘤多位于粘膜下 ,伴有淋巴管浸润及淋巴结转移。结论　CyclinE及 p5 3联合异常表达促进早期胃癌的进展及淋巴结转移。     

CLINICAL USE OF COMBINED DETECTION WITH TUMOR MARKERS FOR PANCREATIC CANCER

Pancreatic cancer is one of the common malig-nant tumors. Its morbidity shows a tendency to in-crease. The prognosis for pancreatic cancer is poorbecause the early--diagnosis for it is difficult.Therefore,every effort should be made to developbetter methods for the early--diagnosis. The serumlevels of CA242,CA19--9 and CA50 of patients withpancreatic cancer were measured by IRMA andwere compared with those of patients with benignpancreatic or biliary tract diseases and with non-.pancreatic…     

EXPRESSION AND SIGNIFICANCE OF C-erbB-2 IN THE TISSUES OF LUNG CANCER

The tyrosine kinase receptor C-erbB-2 (alsocalled HER-2/neu) is a member of the epidermalgrowth factor receptor (EGFR ) family. Memberproteins of this fami1y are membrane proteins andeach of which contains an intracellular domain, atransmembrane domain and an intracellular regionwith intrinsic tyrosine kinase activity. C--erbB-2gene plsay an important role in the development ofcancer. Overexpression of C-erbB-2 can not onlyenhance the ability of the growth and metastaticpotential in cance…     

THE MIB-1 PROLIFERATION INDEX AND MICROVESSEL COUNT IN EARLY GASTRIC CANCER: ASSOCIATION WITH LYMPH NODE METASTASIS

Ki-67 antigen['J, which can be detected usingthe MIBl antibody['J, is a cell proliferation associ-ated nuclear antigen present in all stages of the cellcycle except G,, and thus the proliferation indexdetermined by MIB--1 reflects the cell proliferation.Recently, proliferative activity as evaluated usingthe MIB -l proliferation index (MIB-1 PI) hasbeen shown to be a prognostic factor in several tu-mors[3J. Tumors with low angiogenic activity havea reduced risk of recurrence and metastas…