MORPHOLOGICAL STUDY ON VERTEBARTE MELANOCYTES

ＴｈｅＭｅｌａｎｏｃｙｔｅ（ＭＣ）ｉｓａｔｙｐｅｏｆｄｅｎｄｒｉｔｉｃｃｅｌｌｓｗｈｉｃｈｓｙｎｔｈｅｓｉｚｅｓａｎｄｓｅｃｒｅｔｅｓｍｅｌａｎｉｎ〔１,２〕．Ｓｋｉｎｍｅｌａｎｉｎａｃｔｓａｓａｂａｒｒｉｅｒｔｏｔｈｅｓｕｎｌｉｇｈｔａｎｄｕｌｔｒａｖｉｏｌｅｔｒａｙ．ＩｎｏｒｄｅｒｔｏｅｘｐｌｏｒｅｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆＭＣｉｎｐｈｙｌｏｇｅｎｅｔｉｃｐｒｏｃｅｓｓａｎｄｔｏｍａｋｅａｆｕｒｔｈｅｒｕｎｄｅｒｓｔａｎｄｉｎｇｏｎｔｈｅｆｕｎｃｔｉｏｎｏｆＭＣａｎｄｄｉｓｅａｓｅｓｒ…     

人膀胱移行细胞癌细胞株BLZ-211的生物学特性

以膀胱移行细胞癌患者的肿瘤标本为材料建立了１株克隆性膀胱癌细胞株ＢＬＺ－２１１,体外培养３年多,传代１５０次,没有支原体污染。生物学特性鉴定：光镜和超微结构显示该细胞具有膀胱移行细胞癌细胞特征。细胞接种裸鼠形成的移植肿瘤与手术标本病理形态一致。染色体众数６３,有特异性的ＬＤＨ同功酶谱,有ｐ１６基因的正常扩增条带。结果提示ＢＬＺ－２１１细胞株是一个研究人膀胱移行细胞癌细胞生物学的实用模型。     

粘附分子E-Cadherin和CD_(44v6)表达与肺癌淋巴转移的关系

目的 探讨粘附分子E－Cadherin和CD44v6与肺癌淋巴转移的关系。方法 采用免疫组化SP法检测了63例肺癌组织及其中41例淋巴转移灶中E-Cadherin和CD４４v6的表达。结果 淋巴转移灶中CD４４v6阳性表达率为85..37%,显著高于原发灶(63..49%,P＜0..05);ECadherin和CD４４v6均与肺癌淋巴转移密切相关(P值分别小于0..05和0..001);诊断肺癌淋巴转移的敏感度和特异度E-Cadherin异常表达为63..41%和63..64%,CD４４ｖ６阳性表达为85..37%和77..27%,平行试验敏感度为87..80%,特异度为59..09%,系列试验敏感度为60..98%,特异度为81..18%。结论 E-Cadherin异常表达和CD４４ｖ６阳性表达可能均在肺癌淋巴转移中起重要的促进作用,可作为诊断肺癌淋巴转移的辅助指标。     

THE CHARACTERISTICS OF ENDOGENOUS OUABAIN SECRETIONFROM CULTURED BOVINE ADRENOCORTICAL CELLS

Endogenousouabain(Eo)hasbeenconsideredasacirculatingfactorthatregulatesthebodyfluidvolumebalance,urinesodiumextractionandvaso-contrictivetone,whichplaysanimportantroleinpathogenesisofhypertensionandsomecardiovas-culardisorderst12.Sinceitwasdiscoveredandiden-tifiedbyHamlynin199lt,i,severallinesofevi-dencesindicatethattheadrenalcortexisthemajorsourceofEO.Thepurposeofthisstudyistocom-parethecharacteristicsofEOsecretionwiththewell-knownadrenocorticalhormonesaldosteroneandcortisol,determinetheeff…     

THE CYTOTOXIC EFFECTS OF CRUDE BILE ON HUMAN PANCREATIC CANCER CELL LINES

Thebileacidswereconfirmedasnonspecificcytotoxicsubstanceswhichplayveryimportantro1einpathophysiologyofextrahepaticcholesta-sis[la.Invitrostudieshadprovedthatthebileacidscouldcausechronicandacuteinjuryofpancreaticductalepitheliumactingasaninitiatorofpancre-atitist2i.Thepancreaticcancerisamajorworld-widepublichealthproblernandremainsasignifi-cantclinica1challenge.Atthetimeofdiagnosis,about7o%ofpancreaticcancerpatientsmanifestobstructivejaundicewhichcanresultinprogressiveliverdysfunctionandculmin…     

EXPRESSIONS OF P53, PROLIFERATING CELL NUCLEAR ANITIGEN, BCL-2 PROTEIN AND THEIR SIGNIFICANCE IN SALIVARY ADENOID CYSTIC CARCINOMA

Studiesonthecarcinomasshowedthatthespeedofcarcinomadevelopmentisdependentonthebalanceofcellproliferatingandapoptosis.Soitisveryimportanttostudythemechanismbywhichoncogenescontrolproliferationandapoptosisoftu-morcell.Moststudiesonsalivaryglandtumorshavelaidemphsisontheproliferatingfunctionofonco-genesathomeandabroad,andignoranteditsfunc-tiononapoptosis,especially,thestudieswerefew-.erincombiningtwokindsoffunction.Helen's[13studyonACCindicatedthatmutationofP,3doesn'tplayimportantroleincarcinog…     

褪黑素对链脲佐菌素所致胰岛β细胞损伤的保护作用研究

用链脲佐菌素 (STZ)作用于离体胰岛β细胞 2 4 h,一氧化氮 (NO)、脂质过氧化产物丙二醛(MDA)生成增多 ,胰岛细胞膜流动性下降 ,微粘度增高 ,同时胰岛素分泌功能降低 ,细胞存活率下降。预先给高浓度褪黑素 (10 μmol/ L)处理 2 4 h者 ,可减少 NO、MDA的释放 ,提高超氧化物歧化酶的水平 ,使胰岛细胞的氧化损伤减轻 ,膜微粘度降低 ,膜流动性增加 ,细胞存活率显著提高。但低浓度褪黑素 (10 nmol/ L)无此作用。提示高浓度褪黑素对 STZ所致的胰岛 β细胞损伤有一定的保护作用。     

Two Models of DMFC under Effects of Cathode Humidification Temperature and Anode Flow Rate

This paper introduced a novel self-adjustment of parameters of fuzzy neural networks. Then, the effects of cathode humidification temperature and anode flow rate on the performance of direct methanol fuel celI（DMFC） were described respectively. Two dynamic performance models of DMFC under the influences of cathode humidification temperature and anode flow rate were established separately based on fuzzy neural networks. The simulation results show the accuracy of the models established is satisfactory.     

质子交换膜燃料电池车载系统智能控制算法研究

介绍了质子交换膜燃料电池(PEMFC)车载系统的智能控制算法。由于汽车行驶过程中对功率需求的随机性,使得该系统成为一个变输入变输出系统,对控制的实时性有较高的要求。为此,该系统引入仿人智能控制,并取得了良好的控制效果。     

直接甲醇燃料电池在汽车上的应用前景分析

介绍了直接甲醇燃料电池的结构、工作原理和特性,对影响其应用于汽车的两个主要因素———燃料转换效率和功率密度进行了详细地分析,对目前面临的主要技术难题和解决方法进行了阐述,如催化剂和甲醇渗透等,最后得出了一些相应的结论。