体外简便快速增产大鼠杂交瘤细胞分泌单克隆抗体的方法对比研究

本文报告了以抗肾综合征出血热病毒(HFRSV)大鼠单克隆抗体(McAb)杂交瘤细胞系为模型,研究比较了用常规营养液添加培养法与静置细胞培养法(非添加培养)生产大鼠McAb的产量和滴度的效果。结果表明应用静置细胞培养法,McAb的产量明显高于添加细胞培养法2～3倍,抗体效价亦增加至少一个数量级,并且有简便易行、快速省时、减轻劳动强度和节省原材料的特点,值得试用。     

浅析海上HF数字和电子邮件无线电通信技术

在海上高频(HF)波段使用数字无线电通信技术已经成为全球航运界关注的热点,世界许多国家和有关国际机构正在积极研究。本文对这一通信新技术进行了跟踪和研究,阐述了海上HF数字和电子邮件无线电通信业务;同时对调制解调(MODEM)协议使用的关键技术进行了分析和研究,并提出应对措施。     

碘对神经细胞生长分化蛋白NSE、NGF和IGFR表达的影响

目的　研究碘对体外神经细胞生长发育的直接影响及可能的作用机制。方法　采用体外新生大鼠皮层神经细胞培养法观察微量元素碘对神经细胞表达NSE、NGF和IGFR蛋白的影响。结果　①高浓度碘能增加体外培养的神经细胞表达NSE蛋白 ;②碘在培养的 5、6、7d时NGF蛋白的表达较对照组明显增强 ;③中、高浓度的碘能显著增强体外培养的大鼠皮层神经细胞IGFR蛋白的表达。结论　碘可能是通过促进、增强NSE、NGF和IGFR蛋白的表达 ,进而直接作用于体外培养的神经细胞 ,促进神经细胞的生长、发育与分化     

一种基于H.26L的自适应差错掩盖方法

利用解码帧的特征及其相关参数，提出了一种基于H．26L的自适应差错掩盖方法．该方法对不同的差错特征分别采用以下3种掩盖方法：边界匹配(BMA)、空间插值(SI)和频域插值(FI)．与采用单一方法相比，用本方法的仿真结果的主客观质量都显著提高，尤其是在场景转换和剧烈运动的时候。     

基于工业以太网的隧道施工通风环境参数自动监控系统研究

为提高隧道施工通风系统自动化、信息化水平,提出了一种基于工业以太网络的隧道施工通风监控系统结构框架。系统由上位工控机、下位1200系列PLC及相关模块和传感器组成。工控机中采用Win CC软件编写监控程序,实现隧道施工环境与工作参数的实时监测;采用神经网络算法对现场数据进行学习,得到变频器输出频率的控制规律;1200PLC通过485电缆读取传感器数据;1200PLC和工控机之间通过以太网网线、光纤和无线局域网构成工业以太网络,实现系统环境参数和工作参数的共享。系统在天目山隧道施工通风项目部进行了调试与测试,结果表明:1)1200PLC可以采用485电缆和Modbus协议在掌子面施工环境下顺利读取多个传感器的数据;2)可以采用无线WIFI技术实现衬砌台车两端信息的稳定、可靠传输;3)可以采用神经网络模型实现施工隧道环境参数与风机频率之间的非线性映射。     

Hierarchical model predictive control for multi-lane motorways in presence of Vehicle Automation and Communication Systems

A widespread deployment of vehicle automation and communication systems (VACS) is expected in the next years. This may lead to improvements in traffic management efficiency because of the novel possibilities of using VACS both as sensors and as actuators, as well as of a variety of new communications channels (vehicle-to-vehicles, vehicle-to-infrastructure) and related opportunities. To achieve this traffic flow efficiency, appropriate studies, developing potential control strategies to exploit the VACS availability, are essential. This paper describes a hierarchical model predictive control framework that can be used for the coordinated and integrated control of a motorway system, considering that an amount of vehicles are equipped with specific VACS. The concept employs and exploits the synergistic (integrated) action of a number of old and new control measures, including ramp metering, vehicle speed control, and lane changing control at a macroscopic level. The effectiveness and the computational feasibility of the proposed approach are demonstrated via microscopic simulation for a variety of penetration rates of equipped vehicles.     

Mobility and environment improvement of signalized networks through Vehicle-to-Infrastructure (V2I) communications

Traffic signals, even though crucial for safe operations of busy intersections, are one of the leading causes of travel delays in urban settings, as well as the reason why billions of gallons of fuel are burned, and tons of toxic pollutants released to the atmosphere each year by idling engines. Recent advances in cellular networks and dedicated short-range communications make Vehicle-to-Infrastructure (V2I) communications a reality, as individual cars and traffic signals can now be equipped with communication and computing devices. In this paper, we first presented an integrated simulator with V2I, a car-following model and an emission model to simulate the behavior of vehicles at signalized intersections and calculate travel delays in queues, vehicle emissions, and fuel consumption. We then present a hierarchical green driving strategy based on feedback control to smooth stop-and-go traffic in signalized networks, where signals can disseminate traffic signal information and loop detector data to connected vehicles through V2I communications. In this strategy, the control variable is an individual advisory speed limit for each equipped vehicle, which is calculated from its location, signal settings, and traffic conditions. Finally, we quantify the mobility and environment improvements of the green driving strategy with respect to market penetration rates of equipped vehicles, traffic conditions, communication characteristics, location accuracy, and the car-following model itself, both in isolated and non-isolated intersections. In particular, we demonstrate savings of around 15% in travel delays and around 8% in fuel consumption and greenhouse gas emissions. Different from many existing ecodriving strategies in signalized road networks, where vehicles’ speed profiles are totally controlled, our strategy is hierarchical, since only the speed limit is provided, and vehicles still have to follow their leaders. Such a strategy is crucial for maintaining safety with mixed vehicles.     

Investigating the Interaction Between Gluconobacter Oxydans and Bacillus M egaterium for 2-keto-L-gulonic Acid Biosynthesis in the Two-Step Vitamin C Fermentation

In the two-step vitamin C fermentation process, its precursor 2-keto-L-gulonic acid was synthesized from L-sorbose by mixed culture of Gluconobacter oxydans and Bacillus megaterium. The interaction between Gluconobacter oxydans and Bacillus megaterium remains unclear and it is a challenge to mathematically model the mixed growth of these two strains. The Monod-type equations were previously proposed to describe the coupled growth of Gluconobacter oxydans and Bacillus megaterium. However, in this study, we modeled the interaction of these two strains in a macroscopic view by introducing the population theory. Taking account of the fact that the density or concentration of Gluconobacter oxydans or Bacillus megaterium was hardly to measure accurately in the mixed culture broth, the data of concentrations of the substrate and product were used to indirectly investigate the relation between these two strains. Three batch experiments were used to validate our model. And according to the values of identified parameters, the type of interaction between Gluconobacter oxydans and Bacillus megaterium was concluded to be predation, where Gluconobacter oxydans was predator, and Bacillus megaterium was prey.     

电源零地电压对地铁通信信号系统的影响分析

通过分析地铁通信信号供电系统零地电压的形成机理,论述零地电压产生的不可避免性和对通信信号设备负载可能的影响,建议通信信号系统的使用、设计和工程人员应该正确地看待零地电压问题,走出零地电压的技术误区,避免不必要的资源浪费.     

地铁数据通信系统三种主要无线通信制式的比较

介绍了目前国内城市轨道交通基于通信的列车控制系统中数据通信所采用的无线子系统的三种制式,对这三种制式的特点分别作了描述,着重介绍了TD-LTE(时分-长期演进)制式的一些特点。对这三种主要的无线通信制式,从频率资源、抗干扰性、产品成熟度、自主化程度、成本、数据速率和设备安装等方面进行了对比。