脾虚证大鼠侧脑室注射孤啡肽对细胞免疫功能的影响

目的脾虚证大鼠侧脑室注射孤啡肽,研究其对细胞免疫功能的影响,从神经内分泌的角度探索脾虚的细胞免疫学变化本质。方法用100%大黄煎剂灌胃复制脾虚证大鼠模型,四君子汤作为反证药物,侧脑室埋管,注射孤啡肽,MTT法检测脾T淋巴细胞增殖能力、足跖增厚法检测迟发型变态反应程度,判定细胞免疫功能改变情况。结果灌服大黄煎剂后,大鼠逐渐出现腹泻,或伴有脱肛,食量减少,精神不振,懒动,被毛失去光泽,形体消瘦等脾虚症状,而空白对照组(BC组)没有上述症状;T淋巴细胞增殖能力、迟发型变态反应程度与BC组比较明显减弱(P<0.01)。四君子汤治疗组(DFMD组)和孤啡肽组(OFQ组)大鼠上述脾虚症状及检测指标明显改善(P<0.01)。结论脾虚证大鼠侧脑室注射孤啡肽能提高细胞免疫功能。     

无线射频识别技术在车辆识别系统中的应用研究

介绍了将无线射频识别技术应用于车辆识别系统的方案，提出了基于无限射频识别技术的车辆电子牌照概念。着重讨论基于射频技术的电子牌照及车辆识别系统的应用。并针对不同的应用场录设计程序工作流程。     

智能化航标系统的设计

由于海运业的不断繁荣发展，安全通航尤为重要，这使得航标设备的自动化改造迫在眉睫，计算机的普及、无线通讯风的全球覆盖、蓝牙技术的出现为航标设备的自动化改造创造了条件，使之成为可能。航标设备遥测遥控功能的实现将大大提高航标设备的可利用率，降低维护成本，提高管理效率，具有现实意义。     

Mirror Mapping of Laser Interferometer Measurement System on Ultra-precise Movement Stage

The accuracy and repeatability of the laser interferometer measurement system （LIMS） are often limited by the mirror surface error that comes from the mirror surface shape and distortion. This paper describes a new method to calibrate mirror map on ultraprecise movement stage （UPMS） with nanopositioning and to make a real-time compensation for the mirror surface error by using mirror map data tables with the software algorithm. Based on the mirror map test model, the factors affecting mirror map are analyzed through geometric method on the UPMS with six digrees of freedom. Dam processing methods including spline interpolation and spline offsets are used to process the raw sampling data to build mirror map tables. The linear interpolation as compensation method to make a real-time correction on the stage mirror unflatness is adopted and the correction formulas are illuminated. In this way, the measurement accuracy of the system is obviously improved from 40 nm to 5 nm.     

我国市场化水平与经济增长关系的EBA模型

系统概述了EBA(极限边界分析)模型的概念、性质、检验方法和应用中存在的问题.用EBA模型对我国市场化水平与经济增长的关系进行了实证分析.结果表明,中国经济市场化水平有利于经济增长,但市场化水平的提高在短期内与经济增长无强显著关系.     

基于模糊神经网络的Horn集上的输入归结

关于定理机器证明的归结原理已应用到人工智能的很多领域，同时提出了各种改进方法，其中，输入归结是一种非常好的推理方法，它对于Horn集是完备的，模糊神经网络是模糊逻辑与神经网络的融合，文中利用模糊神经网络的知识表示及学习的特点，结合输入归结的优点，进行Horn集上的输入归结。     

Study of Temperature Field in Natural Ventilated Atrium

Full-scale experimental measurements and modified k-ε two-equation computational fluid dynamics(CFD) model with buoyancy term are used to investigate temperature field of the single-sided natural ventilated atrium with large openings. Compared with the measured values, the modified k--ε turbulent mode can effectively simulate the low Reynold number airflow in the single-side naturally ventilated atrium. Based on the experiment,the optimum period of utilizing natural ventilation for indoor climate control was determined. And the characteristic of the temperature field was analyzed and classified into two zones:lower linear temperature distribution zone and upper thermal accumulation area where temperature is power function distributed. These can be referenced for design and operation of atrium in north China.     

PLASMA SODIUM AND NaCl-SENSITIVE HYPERTENSION

Ｉｎｍａｎｙｉｎｄｉｖｉｄｕａｌｓ ,ｈｉｇｈ ＮａＣｌｄｉｅｔｓｉｎｃｒｅａｓｅａｒｔｅｒｉａｌｐｒｅｓｓｕｒｅ ,ｂｕｔｔｈｅｍｅｃｈａｎｉｓｍｌｉｎｋｉｎｇＮａＣｌｉｎｔａｋｅｔｏｈｙｐｅｒｔｅｎｓｉｏｎｒｅｍａｉｎｓｅｌｕｓｉｖｅ .Ｓｅｖｅｒａｌｌｉｎｅｓｏｆｅｖｉｄｅｎｃｅｓｕｇｇｅｓｔｔｈａｔａｈｉｇｈ ＮａＣｌｄｉｅｔｍａｙｉｎｃｒｅａｓｅ ｐｌａｓｍａｓｏｄｉｕｍｃｏｎｃｅｎｔｒａｔｉｏｎ ,ｔｈｅｒｅｆｏｒｅａｃｔｉｖａｔｉｎｇｃｅｎｔｒａｌｏｓｍｏｓｅｎｓｉｔｉｖｅｎｕｃｌｅｉａｎｄ…     

逆转录病毒介导CXCR4-siRNA对前列腺癌细胞体外侵袭能力的影响

目的设计构建趋化因子受体4(CXCR4)的RNA干扰逆转录病毒载体,探讨干扰CXCR4表达对前列腺癌细胞侵袭能力的影响。方法用PCR扩增CXCR4特异性小干扰RNA(smallinterferingRNA,siRNA),转入带有增强型绿色荧光蛋白(EGFP)和启动子U6的Pgensil-1质粒中,再将重组基因片段导入到逆转录病毒真核表达载体PLX-SN中,行限制性酶切及测序鉴定。重组逆转录病毒载体转染包装细胞PA317,获取病毒上清转染前列腺癌细胞,RT-PCR检测CXCR4mRNA表达。Transwell小室侵袭实验检测前列腺癌细胞体外侵袭能力的变化。结果通过酶切及测序鉴定,成功构建了PLXSN/EGFP-U6-siCXCR4。干扰质粒抑制CXCR4mRNA的表达,与空载体组比较,PC-3m、LNCaPsiRNA对CXCR4mRNA的抑制率分别为(84.26±10.20)%和(88.17±11.23)%。Transwell侵袭实验结果显示,干扰组微膜下孔细胞数PC-3m、LNCaP分别为(14.7±3.1)和(18.9±4.2)个,空载体组分别为(46.9±5.3)和(66.7±6.0)个,两者差异显著(P<0.05)。结论PLXSN/EGFP-U6-siCXCR4可以有效地抑制前列腺癌细胞CXCR4mRNA的表达,干扰CXCR4可以显著抑制前列腺癌细胞体外侵袭转移能力。