拱桥吊杆更换工艺及其力学特性分析

对中、下承式拱桥吊杆主要病害进行统计和原因分析,提出吊杆更换的一般原则,并对新吊杆类型选择、临时替代设施进行对比和分析。以重庆市合川合阳嘉陵江大桥为工程背景,对其吊杆更换工艺和力学特性进行分析,获得了良好效果。     

既有铁路隧道衬砌钢拱架安装槽快速开槽技术综述

采用人工手持混凝土切割机、凿毛机等通用机具进行既有铁路隧道衬砌钢拱架安装槽开槽施工,存在机械化程度低、开槽速度慢、难以满足隧道病害快速整治要求且施工安全难以保证等问题。在调研国内外隧道病害整治开槽方法和设备现状的基础上,基于金刚石锯片切削混凝土属于脆性破坏机制,提出“组合式刀具一次切削成槽”的隧道衬砌钢拱架安装槽开槽方法及施工工艺; 针对水射流切割技术,提出隧道衬砌钢拱架安装槽“水射流切槽”施工工艺; 针对切割轮和环形轨道开槽机,提出“多次切削成槽”方法及施工工艺。基于“组合式刀具一次切削成槽”、“水射流切槽”和“多次切削成槽”3种开槽施工技术,提出既有铁路隧道衬砌钢拱架安装槽开槽设备的研制建议。     

沉管隧道运维技术发展现状综述

为促进沉管隧道运维技术发展,通过文献调研、现场调查与数据挖掘等方法,系统梳理了沉管隧道的国内外概况、运营与病损情况、检测与评估技术、病害预防及处治等方面的技术成果。以沉管隧道用途、管节长度与埋置水深等关键信息为基础,阐释了其国内外发展概况;简述了沉管隧道运营现状,并重点对沉管隧道接头、主体结构、附属结构与设施的常见病损特征进行了数据挖掘;对隧道检测、监测与评估技术进行了对照剖析,阐述了目前国内外检测与评估技术的进步与不足;从前期预防与后期处治的双角度,阐述了沉管隧道的不均匀沉降、裂缝与渗漏水等常见病害的预防与处治;并从沉管隧道运维信息的角度,对其运维技术的发展趋势进行了展望。结果表明：沉管隧道建设已取得丰硕成果,但仍存在诸多运维技术难题;在役沉管隧道出现了过大(差异)沉降、裂缝、渗漏水等病损,其中管节不均匀沉降最为常见;沉管隧道检测仍以结构检测对象和传统周期性检测方法为主;沉管隧道评估诊断多侧重于沉降量控制标准的角度,考虑多因素综合角度的服役性能评估尚处于空白阶段;沉管隧道常见病害应坚持建设期预防和运营期处治双管齐下的对策。未来,沉管隧道运维将向运营环境全要素拓展,并更注重实时自动化监测技术、大数据智能评估技术与基于状态的养护策略等新技术。     

满城顺平界至京赞线交叉口段大修方案

京赞支线满城顺平界至京赞线交叉口段因出现多种病害，日常的养护维修已不能满足行车安全的需求，急需进行大修改造。为此，根据沿线自然地理条件和旧路现状，对路基路面、安全设施、平交、排水等提出了大修方案，以供类似工程借鉴。     

绳锯在高铁路基上拱病害整治中的应用

本文以某高铁路基上拱病害为研究对象,在病害调研及成因分析的基础上,将桥梁、地铁等工程中常用的绳锯切割技术首次引入到高铁路基上拱整治施工中。绳锯静力切割具有切割件大小可随意控制、施工速度快、切割切口平直光滑、吊运方便、对保留部分无振动、无破坏、操作安全、对环境污染小等特点,有效地解决了高铁路基既有线病害整治施工中工期紧张、空间狭小等技术难题,既提高了施工效率,也确保了施工安全,整治效果良好,可为类似病害整治提供参考。     

山区高速公路长大纵坡段车辙病害分析及防治措施

结合山区高速公路车辙病害的调查数据及病害特点,运用粘弹性力学原理分析交通、长大纵坡、高温等外部因素对车辙病害的影响,通过车辙试验说明温度、胎压、作用时间对动稳定度的影响。并从设计、施工、管理等方面提出相应的车辙防治措施。     

基于检测与试验的老龄钢桥结构安全评估

对已运营70余年的老龄钢桥灵桥进行了上部结构病害检测、关键节点和杆件的超声波探伤、拱圈与吊杆空间形位测量、荷载试验,再综合历年检测数据和计算模型,深入分析了灵桥的退化程度和承载能力,得出以下主要结论：在现有交通条件下,灵桥的结构状态暂时是安全的,但其病害加剧,尤其是桥面系破坏严重、拱脚严重腐蚀,致使该桥已不满足使用要求,应尽快维修或改造。     

Aβ诱导PC-12细胞凋亡建立阿尔茨海默病细胞模型

目的　用β 淀粉样蛋白 (Aβ2 5- 35)诱导PC 1 2细胞凋亡以建立阿尔茨海默病细胞模型。方法　体外培养大鼠嗜铬细胞瘤PC 1 2细胞 ,以不同浓度Aβ2 5 - 35诱导并于不同时间收获细胞 ,应用MTT法观察细胞活力 ,Fura 2 /AM荧光分析法检测细胞内游离Ca2 +浓度 ,Hoechst332 5 8及碘化丙啶复染分析细胞凋亡。结果　Aβ2 5- 35作用于PC 1 2细胞后 ,细胞活力逐渐下降 ,并呈时间和剂量依赖性 ;同时细胞内Ca2 +浓度显著上升 ;不同浓度Aβ2 5 - 35作用后 ,PC 1 2细胞于不同时间出现凋亡的典型形态学特征 ,该时间比Ca2 +浓度上升约晚 6～ 1 2h。结论　Aβ2 5- 35诱导后PC 1 2细胞活力下降、细胞内Ca2 +浓度上升及细胞凋亡 ,可作为较理想的阿尔茨海默病细胞模型     

Correlation between endothelia cells activation and imbalance of cytokines in pulmonary hypertension of congenital heart disease

Objective To explore the correlation between endothelia cells activation and cytokines （ET-1, NO） levels in patients with pulmonary hypertension （PH）, and to discuss their roles in the development of PH. Methods Twenty patients with simple ventricular septal defect （VSD） were chosen as controls, and 30 patients with PH were studied. Plasma levels of ET-1 and NO were measured by radioimmunoassay or colorimetric method. Before cardiopulmonary bypass was established, the specimens from right lung were fixed with formaldehyde solution, embedded with paraffin and stained by SP immunohlstochemistry. Intercellular adhesion molecule-1 （ICAM-1） expression was measured through the determination of the light density with computer imaging technology. Results Compared with that of the patients with simple VSD, the light density of ICAM-1 and plasma level of ET-1 increased in patients with PH; but plasma level of NO decreased （P〈0.05）. Positive correlation was observed between ICAM-1 and ET-1/NO （P〈0.05）. Conclusion Endothelia cells activation and imbalance of ET-1/NO might play an important role in the development of PH.     

THE EFFECT OF FCγ RECEPTOR ON THE PATHOGENESIS OF GRAVES'' DISEASE

Objective To explore the roles of Fcγ receptor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO mice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice were immunized by hTSH receptor expressing cells (DAP3.WT).1-2×107 DAP3.WT cells were peritoneally injected into mice every two weeks for a total of six times.Two weeks after final immunization, mice were killed for measurement of total thyroxine,TRAb and pathological examination.Results The thyroxine level of the immunized Fcγ receptor gene knockout mice was significantly lower than that of the immunized wild type control mice (2.2±0.31 vs.3.32±0.59g·dL-1,P<0.05), but there was no significant difference between immunized Fcγ R KO mice and non-immunized wild type control group.The TRAb levels of the immunized Fγ R KO mice significantly increased compared to those of the immunized wild type mice (21.75±8.21 vs.14.11±6.21, P<0.05). The lymphocyte cells infiltration and destruction of thyroid follicles were found in the thyroid gland of the immunized Fcγ R KO mice. Conclusion　These results suggest that Fcγ receptor may be involved in the pathogenesis of Graves'disease.