施工企业投标资料库的建立与完善

投标工作是一项时间紧、任务重、不允许出错的工作。若要提高投标文件的编制质量及效率,必须建立一个充分、完善的资料库为编制工作提供参考。此文从商务资料库、技术资料库和报价资料库三方面对资料库的建设与完善做了较为系统的论述,对施工企业的投标管理工作具有一定的参考价值。     

施工企业统计数据的分析及应用

通过对企业全年经济数据分析,得出企业全年经济运行拐点上扬的趋势。结合国内基础建设现况,指出建设项目按基建程序办事的重要性;针对企业情况,提出企业经济增长需提高内生动力。     

浅析国有企业人力资源管理现状

知识经济时代,市场竞争日趋激烈,人力资源管理不仅对企业的经营与发展起重要的作用,而还起着决定企业命运的战略性作用。分析了国有企业人力资源管理在认识上、用人制度、激励机制、员工沟通等方面存在着不足,从人力资源管理理念、人力资源规划制度、用人原则等方面提出了国有企业人力资源管理的对策。     

EHF病毒在传代鸡胚上的实验观察

本文报道了用已知EHFV seoul株接种9～10d龄鸡胚后鸡胚出现严重局限的出血斑;ELISA试验显示感染鸡胚尿囊液和对照鸡胚尿囊液OD值之比>2.1;免疫荧光试验在感染鸡胚心、脑、肺、肝、肾组织印片细胞浆内可见不同程度荧光颗粒。     

施工企业基于MRP材料管理探讨

在激烈的市场竞争中,施工企业在工程建设中加强内部集约化和精细化管理,采用物料需求计划（MRP）管理是控制工程项目成本的有效手段。此文在阐述MRP采购与管理模式的基础上,针对工程项目建设,详细论述采用MRP管理的工作流程、具体实施步骤及主要内容,以期减少材料的库存量和库存损耗,达到有效降低项目成本的目的。     

建筑企业科技成果网络评审平台构建初探

介绍了如何构建大型建筑企业科技成果评审网络平台模型,阐述了科技成果申报、评审、查询等工作实现网络化的工作原理及工作流程。     

The symbolism of ‘eco cars’ across national cultures: Potential implications for policy formulation and transfer

Transport choices are not merely practical decisions but steeped in cultural and societal perceptions. Understanding these latent drivers of behaviour will allow countries to develop and import policies to more successfully promote sustainable transport. Transport symbolism – what people believe their ownership or use of a mode connotes to others about their societal position – has been shown to be one such, non-trivial, hidden motivator. In the case of hybrid and electric cars (‘eco cars’), studies have demonstrated how their symbolic value varies within a society among different social groups. As yet, however, there has been scant research into comparing how the symbolism of a mode varies across national cultures, horizontally, between individuals with similar socio-demographic characteristics. Through qualitative thematic analysis, this study utilises two of Hofstede’s cross-cultural indices – power differential and individualism versus collectivism – to develop and strengthen theory on how the differing symbolism of eco cars currently varies between four cultural clusters – Anglo, Nordic, Confucian and South Asian. It also deliberates how observed symbolic qualitative differences may influence an individual or group choice to procure eco cars. Finally, it discusses how policy development, transfer and marketing, within the context of eco cars, may need to be modified by national governments, in the Confucian and South Asian cultures, so as to encourage uptake and modal shift.     

Career development for women in maritime industry: organisation and socialisation perspectives

For centuries, the maritime industry has been a recognised place in society dominated solely by men, and even though there were women involved, they were mostly on the periphery. This research aims to fill the gap of women’s career development in this field, by investigating the relationships among the degree of organisational socialisation, women’s workplace culture, and the career development of women in the maritime workplace. The analyses are conducted with the structural equation model with 202 valid questionnaires. The results indicated that women’s workplace culture has a significantly negative relationship with the degree of organisational socialisation, and then in turn, it has a significantly positive relationship with women’s career development in the maritime industry. Finally, the results also indicate that the mediating effect of organisational socialisation existed between women’s workplace culture and women’s career development. According to the empirical analysis, this study makes conclusions as well as suggestions for the maritime enterprises as the reference for human resource management.     

体外大鼠成骨细胞对破骨细胞形成的影响

目的通过将鼠成骨细胞与破骨细胞直接共培养的实验方法,研究成骨细胞对破骨细胞分化及功能成熟的影响。方法按照M-CSF 30μg/L、RANKL 50μg/L的浓度对鼠骨髓单个核细胞诱导培养6 d,与原代培养3 d的鼠成骨细胞按细胞数量1∶1直接共培养,加入1,25-(OH)2D31×10-8mol/L和PGE21×10-6mol/L,利用形态学观察、TRAP染色、骨吸收陷窝检测等方法对共培养细胞进行鉴定。结果成骨细胞与破骨细胞共培养时,成骨细胞有明显的生长优势;染色后显微镜下可见大量呈单层排列的成骨细胞,偶见TRAP( )的破骨细胞。结论成骨细胞对破骨细胞分化及功能成熟的影响与两者的相对数量有关。本实验中,由于成骨细胞快速生长,当其数量多于破骨细胞时,可能使1,25-(OH)2D3对成骨细胞RANKL表达的上调作用不足,且PGE2对成骨细胞OPG表达的下调作用不足,从而主要表现为对破骨细胞形成及分化的抑制作用。     

刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。