c-myc反义寡核苷酸对肾癌细胞的生长抑制作用及对细胞周期的影响

目的　观察修饰的c myc反义寡核苷酸 (ASODN)对肾癌细胞的生长和细胞周期的影响。 方法　用人工合成的c mycASODN转染于培养的人肾癌细胞 ,观察其对瘤细胞的作用。结果　①MTT法测定发现 ,与正义和错配链相比较 ,c mycASODN有明显抑制肾癌细胞生长的作用 ,12 μmol·L-1c mycASODN组作用 4 8h ,对瘤细胞的生长抑制率为 4 7% ;该生长抑制作用随反义药物剂量的增加而增强 ,同时随作用时间的变化而变化。②流式细胞仪检测证明 ,c mycASODN对肾癌细胞周期的抑制作用在G1期到S期。结论　c mycASODN具有序列特异性和细胞周期特异性地抑制肾癌细胞生长作用     

神经网络在车辆半主动控制中的应用

针对车辆半主动悬挂模型的非线性特性，研究了采用神经网络的自适应控制在车辆悬挂半主动控制中的应用，设计了采用前馈神经网络的辩识器和控制器，对控制器网络采用的学习规则进行了分析。仿真计算表明，采用神经网络自适应控制方法能有效改善车辆的平稳性，采用的神经网络辩识器和控制器的改进BP算法是有效的。     

IP ADDRESS AUTOCONFIGURATION FOR WIRELESS AD HOC NETWORKS

IntroductionWireless ad hoc networks are gaining increas-ing popularity in recent years because of their easeof deployment. No wired base station or infras-tructure is supported,and each hostcommunicatesone anothervia packetradios. In ad hoc networks,routing protocols are challenged with establishingand maintaining multihop routes in the face of mo-bility,bandwidth limitation and power con-straints. In ad hoc networks,each node( host)acts as a router since routes are mostly multihop.Nodes in …     

环氧化酶-2及表皮生长因子受体在非小细胞肺癌组织中的表达及临床意义

目的探讨环氧化酶-2(COX-2)与表皮生长因子受体(EGFR)在非小细胞肺癌(NSCLC)组织中的表达及与肺癌发生发展的关系。方法采用免疫组化(SP)法检测56例NSCLC和20例正常肺组织中COX-2与EGFR的表达。结果56例NSCLC组织中,COX-2与EGFR表达阳性率分别为64.2%和67.8%,显著高于正常肺组织(P<0.01),COX-2与EGFR表达与临床分期、病理分级、组织学分型和淋巴结转移密切相关(P<0.05),肿瘤分期愈晚,细胞分化越差,淋巴结转移越高,COX-2与EGFR表达阳性率越高,腺癌中COX-2表达明显高于鳞癌,鳞癌中EGFR表达高于腺癌(P<0.05)。结论COX-2与EGFR在NSCLC中异常表达说明其在肺癌发生发展中有重要作用,可作为判断NSCLC的生物学行为及估计预后的指标。     

质子交换膜燃料电池接触电阻研究进展

质子交换膜燃料电池(PEMFC)接触电阻问题是制约其商业化发展的一个重要因素。影响接触电阻的因素可归纳为电池材料、组装参数、工作参数和其他因素。本文主要介绍了PEMFC的接触电阻及其影响因素,并综述了降低接触电阻的方法。     

Modeling traffic operation at signalized intersections without explicit left‐turn yielding rules with an enhanced cell transmission model

This paper presents an enhanced cell transmission model (CTM) to capture traffic operation at signalized intersections without explicit permissive left‐turn yielding rules (i.e. aggressive permissive left‐turn maneuvers may not necessarily yield to opposing through traffic), which can be widely observed in many developing countries. Different from previous studies that focus on traffic dynamics on approaching links, this study contributes to modeling traffic operations within the intersection. A novel cell transmission framework with various types of virtual cells is proposed to model the dynamics of traffic movements from approach to exit. The unique phenomenon of competitive occupying of the conflict point between the left turn and opposing through movements is modeled. The cell state indicating its blockage is proposed to capture the dynamic queue formulation and dissipation and to evaluate the operational traffic performance at the intersection. Field validation results show that the proposed model can capture the operation of traffic at signalized intersections without explicit permissive left‐turn yielding rules with significantly higher level of accuracy than traditional traffic flow models. Copyright © 2016 John Wiley & Sons, Ltd.     

Practicability study on the suitability of artificial,neural networks for the approximation of unknown steering torques

For steer-by-wire systems, the steering feedback must be generated artificially due to the system characteristics. Classical control concepts require operating-point driven optimisations as well as increased calibration efforts in order to adequately simulate the steering torque in all driving states. Artificial neural networks (ANNs) are an innovative control concept; they are capable of learning arbitrary non-linear correlations without complex knowledge of physical dependencies. The present study investigates the suitability of neural networks for approximating unknown steering torques. To ensure robust processing of arbitrary data, network training with a sufficient volume of training data is required, that represents the relation between the input and target values in a wide range. The data were recorded in the course of various test drives. In this research, a variety of network topologies were trained, analysed and evaluated. Though the fundamental suitability of ANNs for the present control task was demonstrated.     

电动汽车技术发展趋势及前景（下）

4燃料电池电动汽车 早在1839年．英国人格罗孚就提出了氢和氧反应发电的原理。20世纪60年代,研发出了液氢和液氧发电的燃料电池．由美国UTC公司首先用于航天和军事用途。近20年来．由于石油危机和大气污染日趋严重．以质子交换膜为代表的燃料电池技术．受到世界各国普遍重视。各大汽车公司纷纷投入巨资．研发出了各种类型的燃料电池电动汽车（FCEV）．     

燃料电池混合动力汽车动力系统匹配与优化研究

首先,基于中国客车典型循环工况对燃料电池混合动力系统进行匹配计算,确定了电动机、燃料电池发动机和蓄电池的基本参数;然后基于中国客车典型循环工况,建立燃料电池混合动力系统的优化模型,采用序列二次规划算法进行优化,分析了各种参数对整车燃料经济性的影响,包括燃料电池发动机与动力蓄电池之间的功率分配比、SOC的初始值与目标值、变速器传动比及传动比间隔以及主减速比等,为燃料电池混合动力汽车的构型提供指导。     

车用质子交换膜燃料电池性能仿真与试验研究

总结了燃料电池极化曲线的半经验公式,利用某80kW质子交换膜燃料电池试验数据来拟合公式中的相关系数,同时利用Matlab/Simulink软件建立了质子交换膜燃料电池堆的仿真模型,为燃料电池发动机系统的仿真和分析提供了一个重要工具。通过此模型可以研究燃料电池电堆的运行参数如气体压力、温度、当量比等对电堆性能的影响,从而有助于研究整个燃料电池发动机系统的性能。