质子交换膜燃料电池车载系统智能控制算法研究

介绍了质子交换膜燃料电池(PEMFC)车载系统的智能控制算法。由于汽车行驶过程中对功率需求的随机性,使得该系统成为一个变输入变输出系统,对控制的实时性有较高的要求。为此,该系统引入仿人智能控制,并取得了良好的控制效果。     

直接甲醇燃料电池在汽车上的应用前景分析

介绍了直接甲醇燃料电池的结构、工作原理和特性,对影响其应用于汽车的两个主要因素———燃料转换效率和功率密度进行了详细地分析,对目前面临的主要技术难题和解决方法进行了阐述,如催化剂和甲醇渗透等,最后得出了一些相应的结论。     

车用可插拔式燃料电池增程器匹配设计研究

为一款正在开发的燃料电池增程式微型电动车,提出了可插拔式燃料电池增程器/蓄电池动力系统的方案和系统关键部件,如燃料电池、DC/DC转换器等的选型原则和匹配设计方法;为在仿真中考虑排氢动作对风冷自增湿燃料电池耗氢量的影响,提出了瞬时氢气过量系数查表法;最后,利用M atlab/Cru ise联合仿真平台对匹配设计结果进行了验证。     

NANOG基因在宫颈癌中的表达及其意义

目的 研究NANOG在宫颈癌中的表达及其在宫颈癌发生发展中的作用.方法 用免疫组织化学检测正常宫颈、宫颈原位癌及宫颈癌中NANOG的表达;分别用原代宫颈癌肿瘤球细胞及肿瘤球分化细胞接种裸鼠,观察移植瘤的生长情况.结果 与正常组织相比,宫颈原位癌及宫颈癌中NANOG基因的表达显著增强(P<0.05).新鲜宫颈癌组织经过无血清培养可以生成肿瘤球,再次经过血清培养后能够分化成上皮样细胞;裸鼠移植瘤实验证实这种肿瘤球细胞具有很高的成瘤性.结论 宫颈癌组织中存在肿瘤干细胞,NANOG在介导宫颈癌的发生过程中起到重要作用.     

Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells

Objective To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method. The cultured BMSCs were divided into three groups: normal control, H2O2 treatment (100μmol/L), and PNS pretreatment (0.1g/L). Intracellular reactive oxygen species (ROS) levels as the index of oxidative stress were measured by using 2'7'-dichlorodihydrofluorescein diacetate. Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI. The protein expression of Bax in BMSCs was analyzed by Western blotting. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2 (P<0.01). PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P<0.01). PNS reversed H2O2-induced augmentation of Bax expression. Furthermore, PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P<0.01). Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.     

Association between the -1562 C/T polymorphism in the MMP-9 promoter and phenotype of esophageal squamous cell carcinoma in northern Chinese population

Objective To conduct a case-control study on the association of the nucleotide polymorphisms in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene with phenotype of esophageal cancer. Methods All subjects were unrelated residents in northern regions of China. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the MMP-9 genotypes. Results The overall distribution of genotypes in the patients was not different from that in the controls (OR=0.77, 95% CI=0.45-1.34; P=0.36). There were no significant differences between the patients and the control subjects in terms of the distributions of sex and age, smoking status, alcohol dependence, pickled diet status, or history of environmental exposure. The patients were further examined with stratifications by age, sex, grade, depth of tumor invasion, lymphatic invasion, venous invasion and TNM staging. The results showed no pronounced association among the stratifications. Conclusion There is no significant association between the MMP-9 single nucleotide polymorphism genotypes and phenotype of esophageal cancer.     

基于手机定位的交通OD数据获取技术

居民出行OD数据是交通规划中最重要的基础资料之一。传统的利用纸和笔、电话调查以及路边询问等调查方式虽然简单,但存在较大局限性。随着信息技术、移动技术等的发展,手机信息和定位技术应用于交通数据采集是研究的热点之一。在CDMA通信网络和gpsOne手机定位技术的基础上,对出行者进行连续位置跟踪,通过数据处理提取出行者的起讫点位置信息,再结合已有交通区划分,根据点与多边形关系的算法中的面积判断算法得到出行者的OD信息。最后利用所提出的方法对重庆市九龙坡区杨家坪地区进行了OD调查,得到OD发生集中专题和OD线专题。     

驾驶中拨打手机对驾驶人脑力负荷及驾驶行为的影响分析

基于驾驶模拟实验平台,选择高速公路、城市道路2种交通环境,对3名低龄驾驶人正常驾驶与拨打手机驾驶情况下的脑力负荷及驾驶行为表征指标进行了全程监测。每组实验时间约为15min。其中,脑力负荷评估采用心率变异性相关指标,驾驶行为表征采用后视镜使用、转向灯使用、档位变换及速度变化等指标。实验结果表明,相比于正常状态驾驶中拨打手机时心率变异性指标,出现了驾驶人LFNU指标增大、HFNU指标减小、LF/HF比值明显增大、TP增大的现象,规律明显。在排除驾驶疲劳对心电指标产生影响的可能后,可初步判定出现以上现象的原因是拨打手机引起了驾驶人脑力负荷的大幅增加。在驾驶人认知资源有限的情况下,脑力负荷的增大造成了驾驶人在信息获取、转向灯使用、档位变换和车速保持等方面的能力有不同程度的下降。综上所述,驾驶中拨打手机使得驾驶人脑力负荷大幅增加,进而对驾驶行为产生了诸多负面影响,给交通安全带来了隐患。因此,建议相关部门在法律、法规中对驾驶中拨打手机的行为加以限制,以减少交通事故风险。     

基于FLUENT的高温PEMFC流场设计

与传统低温燃料电池相比,高温PEM燃料电池可简化燃料电池的水管理和热管理系统,降低系统的复杂性和成本,近年来高温PEMFC的流场设计逐渐成为研究的重点.基于计算流体软件FLUENT中的PEMFc模块,对3种常见的流场形式分别从膜中水舍量、氧气的浓度和电流密度分布3个方面进行了三维数值模拟和综合分析.结果显示,在高温条件下,性能由高到低依次为：单蛇形流场、平行流场和多蛇形流场.该方法可用于高温PEM燃料电池流场的设计指导.     

天津响螺湾海河开启桥枢轴节点有限元分析

该文利用有限元软件ABAQUS对天津市响螺湾海河开启桥箱梁整体模型进行了静力分析。分别采用不同单元类型、不同网格尺寸对枢轴所在节间建立了a、b、c、d四个子模型,对子模型进行线弹性分析。将分析结果与全局模型分析结果进行对比分析,讨论因为网格尺寸、单元类型对于数值模拟分析的影响。