p57kip2和p21cip1在人脑胶质瘤中的表达和临床意义

目的探讨p57kip2蛋白、p21cip1蛋白在人脑胶质瘤中的表达情况及其临床意义。方法采用免疫组织化学SP法检测p57kip2、p21cip1在55例人脑胶质瘤和10例正常脑组织中的表达。结果在55例胶质瘤中,p57kip2蛋白阳性率为38.2%(21/55),显著低于正常脑组织的80%(8/10)(P=0.036)。p57kip2蛋白表达在低度恶性(Ⅰ-Ⅱ级)组显著高于高度恶性(Ⅲ-Ⅳ级)组(P=0.029),生存时间≥2年组显著高于<2年组(P=0.032)。脑胶质瘤中p21cip1蛋白阳性率为60.0%(33/55),与正常脑组织的90.0%(9/10)相比,差异无统计学意义(P=0.143)。p21cip1蛋白表达在高度恶性(Ⅲ-Ⅳ级)组显著低于低度恶性(Ⅰ-Ⅱ级)组(P=0.047),生存时间<2年组显著低于≥2年组(P=0.002)。p57kip2蛋白的表达与p21cip1蛋白的表达存在正相关(rs=0.354,P=0.008)。结论p57kip2蛋白和p21cip1蛋白在脑胶质瘤中存在异常表达,p57kip2蛋白和p21cip1蛋白的表达程度与脑胶质瘤的分化程度、预后相关。     

p21WAF1/CIP1基因及p53蛋白在大肠癌的表达及意义

用免疫组织化学方法对 4 2例大肠癌组织 p2 1WAF1/ CIP1基因及 p53蛋白的表达进行检测。结果表明 :p2 1WAF1/ CIP1阳性率 4 2 .9% ,p53蛋白阳性率 6 4 .1% ;两者与大肠癌的组织分化、淋巴结转移及术后生存率之间有关 ,并随着肿瘤恶性程度的增加 ,p2 1WAF1/ CIP1阳性率下降 ,p53蛋白阳性率增加。推测 p2 1WAF1/ CIP1及 p53之间存在着一定的负性相关关系 ,同时检测 p2 1WAF1/CIP1基因及 p53蛋白的表达情况 ,有助于判断大肠癌的生物学行为及患者的预后。     

桂枝茯苓丸对荷瘤鼠P21^waf/cip基因表达的影响

目的 探讨桂枝茯苓丸诱导肿瘤细胞凋亡的机制,为桂枝茯苓丸(GFW)开发应用提供实验依据.方法 计算抑瘤率,流式细胞仪测定细胞凋亡率,免疫组化法测定P21waf/cip蛋白表达,原位杂交法检测Survivin mRNA表达,电镜观察肿瘤细胞超微结构.结果 GFW抑瘤率为38.93%,流式细胞仪检测GFW组凋亡率17.79%,与模型组比较,差异有统计学意义(P<0.05).GFW上调肿瘤细胞P21waf/cip蛋白表达,下调Survivin mRNA表达.GFW组镜下可见瘤细胞以凋亡变化为主.内膜结构完好,核膜清晰,细胞核固缩,染色质团块状散布核内或边集核膜下,并可见凋亡小体.结论 GFW诱导肿瘤细胞凋亡,其机制可能与上调P21waf/cip及下调Survivin mRNA表达密切相关.     

Logistics connectivity considering import and export for Chinese inland regions in the 21st-Century Maritime Silk Road by dry ports

This investigation studies the logistics connectivity of inland Chinese provinces along the 21st-Century Maritime Silk Road (MSR) from the perspective of the oriented cooperation relationship between seaports and dry ports in inland regions. The logistics connectivity is measured based on the import and export data. First, complex network theory is used to develop a system for evaluating logistics connectivity of inland regions, involving metrics of susceptible-infected-recovered, and improved closeness centrality proposed to drawing on the characteristics of the studied network based on closeness centrality. Second, based on collected data, 136 dry ports in China and 127 seaports along the MSR have been used to construct an empirical network that covers 15 inland provinces of China. Then, the accessibility and importance of inland provinces are analyzed, and key points that serve the improvement of the logistics connectivity of inland provinces are mined and summarized. An example of an improved scheme for the connectivity of inland provinces is presented to demonstrate the application of these key points. The conclusions drawn support decision-making for local governments to improve the logistics connectivity of inland regions in the international logistics network, and then strengthen the participation of inland provinces in the MSR.     

MIKE21软件在滨州港内泊稳研究中的应用

针对海港港区拦沙堤平面主尺度对港内波浪要素（波高、周期等）的影响,通过MIKE21数值模拟的方法,研究渐变式潜堤掩护下的港内泊稳情况,得出不同重现期波浪条件下各计算点的设计波要素。结果表明：浪向与航道走向比较接近时,波浪受航道折射影响较大,航道内比波高明显减小,而两侧比波高增大;浪向与航道的夹角在20°左右时,随着航道与边坡的水深差增大,向航道边坡折射的波能增强;波向与航道夹角42°左右时,航道对波能传播的影响不明显;设计高水位2a一遇波浪作用下,港内泊稳条件较差,且现有方案泊稳不满足规范要求。     

LF21铝合金厚板搅拌摩擦焊接接头微观组织研究

针对14 mm厚的LF21铝合金,采用搅拌摩擦焊进行焊接,焊后对接头的宏观形貌、微观组织和接头不同区域的显微硬度进行分析.实验结果表明:在焊接速度v为200mm*min-1,转速ω为1200rad*min-1时,焊缝成型较美观,焊缝内部无空洞、裂纹等缺陷.显微组织观察发现焊核区的组织为再结晶等轴晶粒,晶粒明显细化;热机械影响区组织由原始母材细纤维组织转变为具有一定弧度的弯曲粗纤维组织;热影响区范围较窄,晶粒与母材相似,但出现了晶粒粗化现象.显微硬度实验发现,焊缝中层和上层的显微硬度较高,而下层较低.     

江苏如东人工岛工程对周边海域水动力的影响

利用MIKE21软件水动力模块建立和验证了西太阳沙二维潮流数学模型,并将该模型应用到工程项目中,分析了工程前后水动力条件的变化。应用结果表明:人工岛方案实施后整体流场基本不变,即使出现流速、流向的变化也仅局限在人工岛周围的局部区域,对烂沙洋深槽流场不会产生影响。因此,在现状纵横岸线边界条件下,实施人工岛工程后,要维持工程附近的滩槽稳定,潮流优势是关键,所以在实施任何建港或围垦工程时都必须以维持现状水流特征和动力环境为前提,应尽量消除不稳定因素,以保证烂沙洋海域滩槽的稳定。     

长江口海域波浪场模拟研究

采用MIKE21 SW波浪谱模型对长江口海域在凤凰台风期间的波浪场进行了模拟计算,考虑了水位和流速的影响,模拟结果和实测吻合较好,能够反映长江口海域的波浪分布。结果显示出水位对波高的影响是比较明显的,高水位条件下深水航道两侧和坝田区波高达到1 m以上,为泥沙的再悬浮提供了动力。     

The role of dry port in hub-and-spoke network under Belt and Road Initiative

Dry port plays increasingly an important role in the integration of inland regions with seaports, and cross-border inland ports especially in the context of Belt and Road Initiative (BRI) originally proposed by China. This paper studies a logistics network connecting the inland regions by dry ports based on a two-stage logistical gravity model. First, a basic logistical gravity model is developed to analyze the radiated inland regions from dry ports, where the logistical quality of dry ports is calculated by principal component analysis. Second, considering mutual impacts among dry ports, seaports and cross-border inland ports, the influence of logistical gravity on the network is examined by using a coefficient based on the Ordered Weighted Averaging Operator in multi-attribute decision theory. An improved logistical gravity model is further developed to investigate the logistical connections among various ports (e.g. dry ports, seaports and cross-border inland ports; hub and feeder ports). Then, a hub-and-spoke network can be established. Dry ports are potential to connect to the Silk Road Economic Belt and the 21st-Century Maritime Silk Road. So a Chinese case is used to verify the proposed method. The strategies of embedding inland regions in the BRI are discussed based on the experimental studies.     

强浪条件下半掩护港区波浪场数值模拟

以以色列南部新建Ashdod码头为工程背景,在分析港区自然条件的基础上,利用二维河口与海岸模拟软件MIKE21,建立了基础方案各个施工阶段港内泊稳模型。通过对不同施工阶段下半掩护港区波浪场进行数值模拟分析,掌握了港内波浪场的分布情况,确定港内正常施工时防波堤口门处的容许波浪要素。根据防波堤口门处的波浪统计资料,得到港区不同区域的最大作业波高标准以及港区不同区域不同施工阶段下全年不可作业的天数。研究结果为工期的合理安排和作业窗口的确定提供依据,并提出了科学合理的施工工序建议。