REGULATED PHOSPHORYLATION OF THE GATA－2 DNA BINDINGPROTEIN IN ENDOTHELIAL CELLS

ＲＥＧＵＬＡＴＥＤＰＨＯＳＰＨＯＲＹＬＡＴＩＯＮＯＦＴＨＥＧＡＴＡ－２ＤＮＡＢＩＮＤＩＮＧＰＲＯＴＥＩＮＩＮＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＳＢｕＸｉｎＥ．Ｅ．Ｑｕｅｒｔｅｒｍｏｕｓ，Ｔ．Ｑｕｅｒｔｅｒｍｏｕｓ（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃｉｎｅ...     

综合治疗舌鳞癌的临床与病理观察(附24例分析)

讨论了舌鳞状细胞癌平阳霉素单一化疗前后的病理组织学对照及化疗后肿瘤局部出现明显的临床愈合现象对手术治疗效果、生存率、生存质量的影响。治疗方法采用肌注或静脉滴注,动脉插管灌注PYM,每日10mg,效果显著。手术前瘤体明显缩小、消失,手术后病理组织学发现肿瘤细胞数量减少,残留细胞退变并被大量淋巴、巨噬细胞及炎性细胞包围,似疤痕化,确保原发灶完整干净切除。     

SUPPRESSION OF GROWTH OF A HUMAN LIVER－DERIVED CELL LINE BY ENDOGENOUSLY PRODUCED PARATHYROID HORMONE－RELATED PEPTIDE

ＳＵＰＰＲＥＳＳＩＯＮＯＦＧＲＯＷＴＨＯＦＡＨＵＭＡＮＬＩＶＥＲ－ＤＥＲＩＶＥＤＣＥＬＬＬＩＮＥＢＹＥＮＤＯＧＥＮＯＵＳＬＹＰＲＯＤＵＣＥＤＰＡＲＡＴＨＹＲＯＩＤＨＯＲＭＯＮＥ－ＲＥＬＡＴＥＤ　ＰＥＰＴＩＤＥＬＩＨｕｓｏｎｇ；Ｐ．Ｋ．Ｓｅｉｔｚ，Ｐ．...     

云南锡业矿工肺癌死亡相关因素分析

近年来的研究表明，云南锡业公司矿工的肺癌发生率和死亡率均高于一般人群，肺癌已成为严重危害工人健康的疾病。已往的研究表明肺癌的发生与矿工的职业暴露有较强的相关，但有关肺癌死亡与职业暴露等因素的研究国内外报道甚少，本文从云锡公司矿工的死亡状况的分析来探讨一下作业过程中劳动条件与肺癌死亡之间的联系，并对因肺癌而导致的减寿年数进行计算和分析。结果表明，高危人群列死因顺位首位的疾病为恶性肿瘤，占死因构成的３４．６８％ ，其死亡率为４０７．４９／１０ 万；肺癌死亡者占恶性肿瘤死亡者的７６．５０％ ，其死亡率为３１１．９０／１０ 万。经多因素Ｌｏｇｉｓｔｉｃ 分析，与肺癌死亡有关的因素有：慢性支气管炎、砷暴露和氡子体的暴露，其中氡子体的暴露又为最主要的相关因素。因肺癌导致的减寿年数为２６２ 人·ａ，平均减寿年数为４．８５ ａ。故开展肺癌的防护对保护职业人群的健康具重要意义。     

Development of fuel cell hybrid powertrain research platform based on dynamic testbed

An experimental research platform based on a dynamic testbed is developed and applied for fuel cell hybrid powertrain integration and control. A driver brake model is added to the dynamic testbed to simulate the braking process of an electric vehicle. Sub-systems of the fuel cell hybrid powertrain are tested, and characteristic parameters are obtained. A simulation platform is constructed in LabVIEW environment, and its validity is verified by dynamic test results. A real time control system is developed with an embedded PC for the function of rapid control prototyping. Using this platform, fuel cell battery hybrid and fuel cell supercapacitor hybrid configurations are investigated. This platform provides a powerful tool for fuel cell powertrain research and development.     

Effects of coolant channels on large-scale Polymer Electrolyte Fuel Cells (PEFCS)

Fully coupled simulations of two-phase transport in Polymer Electrolyte Fuel Cells (PEFCs) and heat transfer in coolant channels are performed in order to investigate the effects of cooling channel configuration on the distributions of temperature and water within PEFCs. When a practical coolant flow rate is applied to large-scale cells for automotive applications, a significant coolant temperature rise is expected from the coolant inlet to the outlet, particularly under high current density operations, creating a significant cell temperature gradient along the flow direction as well. Consequently, a two-phase water profile resulting from evaporation-condensation processes inside PEFCs is also strongly influenced by the cell temperature gradient from the hot coolant inlet toward the cold coolant outlet regions, demonstrating that both temperature and liquid saturation strongly depend on the thermal gradient along the coolant flow path.     

增强型绿色荧光蛋白标记的Hela细胞株的建立

目的构建增强型绿色荧光蛋白(EGFP)标记的Hela细胞系。方法PCR和DNA测序鉴定pEGFP-C1真核表达质粒,采用Qiagen tip 500进行pEGFP-C1真核表达质粒DNA的提取和纯化;cellfectin转染Hela细胞,G418筛选,有限稀释法单克隆培养,荧光显微镜进行荧光检测EGFP的表达,采用激光共聚焦显微镜观测器检测荧光特性。结果PCR和DNA测序证实pEGFP质粒结构正确;在倒置荧光显微镜下,转染24h后,部分Hela细胞可见绿色荧光,转染72h时,大约80%Hela细胞内均可见绿色荧光。加入含G418的选择性培养基进行选择性培养,选择荧光较强的Hela细胞经有限稀释,持续筛选及克隆化培养,获得稳定表达绿色荧光的Hela细胞克隆,扩大培养并传至10代以上,将此细胞株命名为Hela-EGFP。激光共聚焦显微镜观察发现:在蓝光(-395nm)激发时,Hela-EGFP细胞绿色荧光激发波长为395nm,最大发射峰为509nm。结论Hela-EGFP细胞株具备稳定表达EGFP的能力,为实时可视化进行宫颈癌侵袭转移机制的研究奠定了基础。     

原发性支气管肺癌中PTEN基因的蛋白表达及意义

目的研究抑癌基因PTEN在原发性肺癌中的蛋白表达及其临床意义。方法应用免疫组织化学SP法检测56例手术切除的肺癌石蜡标本及3例正常肺组织中的PTEN基因的蛋白表达情况。结果①正常肺组织中PTEN蛋白表达的阳性率明显高于肺癌患者,而在小细胞肺癌中的阳性表达低于非小细胞肺癌;②在56例肺癌患者中,随着分化程度的降低,PTEN的蛋白阳性表达率也随之下降;③Ⅰ、Ⅱ期肺癌患者的PTEN蛋白阳性表达率高于Ⅲ、Ⅳ期患者;④在19例有转移的肺癌患者中,PTEN表达阳性率明显低于无转移的患者。结论PTEN基因的表达与肺癌的病理类型、组织的分化程度、临床分期以及有无淋巴结转移密切相关。     

Symptom monitoring in treatment of cancer patients

Objective To examine self-reported symptoms by the patients receiving cancer therapy, and find out the symptoms that should be coped with and managed during the treatment. Methods A pilot study was conducted on self-reported symptoms on 185 patients receiving chemotherapy and/or radiotherapy for different cancers. The Therapy-Related Symptoms Checklist (TRSC) was used. Results Severe symptoms on the TRSC subscales: loss of appetite,feeling sluggish, weight loss, nausea and hair loss, were reported by the patients. The frequently reported symptoms by those on chemotherapy were nausea, feeling sluggish, weight loss, vomiting, and taste change. The frequently reported symptoms by those on radiotherapy were feeling sluggish, weight loss, loss of appetite, difficult sleeping, and changing taste. The symptoms of loss of appetite, feeling sluggish, weight loss, hair loss, and nausea were both frequently reported by those on radiotherapy and those on chemotherapy. Conclusion Symptom monitoring may be facilitated by TRSC, based on the severity and frequency of reported symptoms, more patients and caregivers could know which symptoms should be preferential interventions.     

燃料电池锂电池混合动力船舶建模与仿真

全电动绿色船舶除了使用电力,还越来越多地采用替代能源,如燃料电池、太阳能电池等。本文建立船舶混合动力推进系统,采用燃料电池和锂电池并联作为混合动力。在功率不足时,锂电池提供额外的功率以满足负载需求。为了模型比较贴合实际,使用真实数据对混合动力系统的子部件进行建模,生成Simulink模型并进行仿真。