DETERMINATION OF URINE TUMOR NECROSIS FACTOR, IL-6, IL-8 AND SERUM IL-6 IN PATIENTS WITH HEMORRHAGIC FEVERS WITH RENAL SYNDROME

Objective To explore the roles of cytokines in the pathogenesis of hemorrhagic fever with renal syndrome（HFRS）. Methods Double-antibody sandwich ELISA was used to determine serum interleukin （IL）-6, urine tumor necrosis factor （TNF）, IL-6 and IL-8 levels in 56 patients with HFRS. Results Serum IL-6, urine TNF, IL-6 and IL-8 concentrations in HFRS patients were significantly higher than those in control group, respectively （P〈0. 001）. The concentrations increased at fever stage, then continued to increase during hypotension stage and peaked at oliguria stage. The concentrations of serum IL-6, urine TNF, IL-6 and IL-8 increased in accord with the severity of the disease and differed greatly among different types of the disease. Serum IL-6 had remarkable relationships with serum specific antibodies. It was positively related to serum β2 -microglobulin （β2 -MG）, blood ureanitrogen （BUN） and creatinine （Cr）. Significant positive relationships were also found both between urine IL-6 and TNF, and between IL-6 and IL-8 （r=0.5768, P〈0.05; r=0. 3760, P〈0.01）. Conclusion TNF, IL-6 and IL-8 activated during the course of the disease. IL-6 is associated with the immunopathological lesions caused by the hyperfunction of humoral immune response. IL-6, IL-8 and TNF are involved in the renal immune impairment. Determining them might, in certain extent, be used in predicting the prognosis and outcome of patients with HFRS.     

GnRH-a在多囊卵巢综合征诱导排卵中的应用

报道3例多爱卵巢综合征（polycysticovarysyndrome，PCOS）患者用克罗米酚（clomiphenecitrate，CC）及人绝经期促性腺激素（humanmenopausalgonadotropin，hMG）促排卵失败，而采用促性腺激素释放激素激动剂（gonadotropinreleasinghormoneagonist，GnRH－a）长期脱敏万案。达到垂体抑制后联合用hMG-人绒毛膜促性腺激素（hCG）促排卵。3例患者各治疗1周期，2例妊娠，1例继续治疗。结果表明：对传统的hMG促排卵疗法无效的PCOS患者，选用GnRH－a联合hMG可获得较好效果；采用GnRH－a长期脱敏万案可减轻卵巢过度刺激综合征（ovarianhyperstimulationsyndrome，OHSS）的发生，减少治疗作废率；但是PCOS患者需用较长时间的GnRH－a才能达到垂体抑制；用GnRH－a＋hMG＋hCG诱导排卵后可出现黄体功能不足，应给hCG或孕酮支持黄体。     

Hallemann—Streiff综合征8例报告

1980年～1988年共遇到8例HalIermann-Streiff综合征,2例进行了7年后随访。病人包括男性6例,女性2例,年龄在8岁～32岁之间。临床表现为:①鸟面畸形;②先天性白内障或无晶体在全部病例中均具备;③牙齿异常7例;④体儒4例,其中1例(例7,男,32岁,身高1.23m,体重29.5 kg)前囟门未闭;⑤皮肤萎缩7例,萎缩灶发亮菲薄,特别在鼻尖部,3例有1～2块斑秃;⑥毛发稀少8例;⑦双侧小眼球6例。①和②对本征的诊断具有重要意义。     

原发性肾病综合征患儿白细胞介素-4、γ-干扰素与激素疗效的关系

目的探讨原发性肾病综合征患儿治疗前外周血白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)与激素治疗效应的关系。方法31例原发性肾病综合征患儿于治疗前抽取静脉血分离单个核细胞,加入植物血凝素刺激其生长,取其上清液,用酶联免疫吸附双抗夹心法检测IL-4、IFN-γ,激素治疗8周后根据治疗反应分为激素敏感组(17例)及激素耐药组(14例)。20例健康儿童作为对照组。结果原发性肾病综合征中激素敏感组治疗前外周血IL-4水平明显高于激素耐药组(P<0.01);IFN-γ水平在激素敏感组及激素耐药组之间差异无统计学意义(P>0.05)。结论测定肾病综合征患儿治疗前外周血中的IL-4可以作为预测激素疗效的一项指标。     

消化道类癌的外科治疗(附15例报告)

本文报道我院1965年至1983年诊治的15例消化道类癌。其中有直肠类癌6例(40%),胃类癌5例(33.3%),横结肠、胰腺、胆囊,阑尾类癌各1例。其临床表现各有特色。外科治疗的手术方式主要取决于肿瘤的部位、大小、浸润的深度和有无淋巴结转移。对合并有类癌综合征的患者,除切除原发灶和转移灶外,还应辅以化疗及抗5—HT药物来消除其症状。     

脂肪细胞因子chemerin与代谢综合征的关系

目的通过检测脂肪细胞因子chemerin在代谢综合征(metabolic syndrome,MS)大鼠脂肪组织及血清中表达水平的变化,探讨其与MS的关系。方法将60只SD大鼠随机分为代谢综合征模型组(MS)和正常对照组(NC),采用高脂、高糖、高盐饲料联合腹腔注射链脲佐菌素的方法构建MS大鼠模型。采用RT-PCR方法检测两组大鼠脂肪组织中chemerin mRNA的表达情况;采用酶联免疫吸附实验检测大鼠血清chemerin蛋白的表达变化。结果①在大鼠肾周围、附睾旁及肩胛间肌旁部位脂肪组织中,chemerin mRNA的表达水平MS组较NC组增高,且差异有统计学意义(P<0.05),在网膜脂肪组织中MS组较NC组虽有升高的趋势,但差异无统计学意义(P>0.05)。②MS组大鼠血清chemerin水平较NC组有升高趋势,但差异无统计学意义(P>0.05)。结论 Chemerin在高脂、高糖、高盐饮食联合STZ构建的MS大鼠模型的发病过程中可能起重要作用。     

Comparative Immune Efficacy of Native Inactivated and Attenuated Vaccines for Porcine Reproductive and Respiratory Syndrome Virus over 2 Consecutive Years

Porcine reproductive and respiratory syndrome(PRRS) was first described in the United States in1987. This syndrome was characterized by abor-tion, premature farrowing, stillborn and mummi-fied pigs, as well as respiratory disease with chron-ic poor performance of nursing and weaned pigsand death. PRRS has been recognized worldwideand is considered to be an economically importantglobal disease since its emergence[1]. Several pro-tocols such as enhancing management, quarantine,disinfection, …     

彩色多普勒血流会聚法对二尖瓣返流定量的评价

为探讨血流会聚新方法对二尖瓣返流定量诊断的临床价值 ,采用脉冲多普勒法作对照 ,研究36例二尖瓣返流患者。结果表明血流会聚法返流量与脉冲多普勒计算返流量具有良好相关性 (r=0 .81～ 0 .94 ,P <0 .0 0 0 1)。血流会聚法计算二尖瓣返流量合适的混叠极限速度为 4 8cm/ s左右。认为血流会聚法是一种准确、可靠且实用的二尖瓣返流量计算方法。     

赖氨布洛芬的解热镇痛和抗炎作用

本(?)对赖氨布洛芬(Ibuprofen-Lysine IL)的药理研究表明:IL25、50mg/kg iv 或10、25 mg/kg im 对伤寒-副伤寒甲乙混合菌苗致热家兔均有解热作用,且强于安乃近。对酒石酸锑钾性小鼠扭体反应,IL25、50 mg/kg sc 均有明显抑制作用;连续7天给药对其镇痛作用不产生明显耐受性。在2天内给小鼠7次scIL,累计剂量达350或525 mg/kg 后,再sc 烯丙吗啡50 mg/kg 催(?),小鼠均无跳跃反应等戒断症状,初步说明IL 无吗啡样的依赖性.IL25、50、100 mg/kg sc 对小鼠角叉菜胶性足跖炎症、二甲苯性耳壳炎症及醋酸性腹膜炎症均有明显抗炎作用.     

病毒性肝炎患者血浆前列腺素和血栓素的检测

本文对我院1988年～1990年100例各型病毒性肝炎患者以放免法检测血浆6-keto-PGF_(10)和TXB_2。其结果显示血浆中PGF_(10)、TXB_2在肝炎患者中均升高,PGF_(10)/TXB_2比值降低,与正常人有明显差异。对两者变化原因与黄疸并发症的相关性进行了分析。并提出两者在肝炎的发病机理中的作用。