体外简便快速增产大鼠杂交瘤细胞分泌单克隆抗体的方法对比研究

本文报告了以抗肾综合征出血热病毒(HFRSV)大鼠单克隆抗体(McAb)杂交瘤细胞系为模型,研究比较了用常规营养液添加培养法与静置细胞培养法(非添加培养)生产大鼠McAb的产量和滴度的效果。结果表明应用静置细胞培养法,McAb的产量明显高于添加细胞培养法2～3倍,抗体效价亦增加至少一个数量级,并且有简便易行、快速省时、减轻劳动强度和节省原材料的特点,值得试用。     

血液净化对肾综合征出血热炎症介质的清除作用

目的　通过观察连续性肾替代治疗 (CRRT)对肾病综合征出血热 (HFRS)患者肿瘤坏死因子α(TNF α)、白介素 6(IL 6 )及内皮素 1(ET 1)的清除作用 ,探讨炎症介质在HFRS中的作用。方法　将诊断为HFRS的中型及以上类型患者 4 0例随机分为两组 :CVVH组 2 0例行CVVH ,HD组 2 0例行HD。用ELISA法检测TNF α和IL 6浓度 ,放射免疫法测定ET 1浓度。结果　①CVVH组及HD组少尿天数 (3.0± 2 .1,6 .0± 3.4 )、并发症发生率 (2 5 % ,4 0 % )及病死率 (15 % ,2 5 % )差异均有显著性 (P <0 .0 1)。②两组治疗前后血肌酐和尿素氮下降有显著性差异 (P <0 .0 1)。③CVVH组治疗后血中TNF α、IL 6和ET 1均较治疗前明显降低 ,差异均有显著性意义 (P均 <0 .0 1) ;HD组治疗前后血中TNF α、IL 6和ET 1无显著性差异 (P >0 .0 5 )。④CVVH组滤出液中可持续检测到IL 6及ET 1,未检测到TNF α。透出液中未检测到上述炎症介质。结论　连续性血液净化治疗可清除炎症介质 ,改善HFRS的病理生理过程 ,改善肾功能和预后 ,减少并发症 ,降低病死率 ,是治疗HFRS的最佳方法     

Viewpoints of adults with and without Autism Spectrum Disorders on public transport

BackgroundPublic transport is low cost, allows for independence, and facilitates engagement and participation for non-drivers. However, the viewpoints of individuals with cognitive disabilities are rarely considered. In Australia, the prevalence of Autism Spectrum Disorders (ASD) is approximately 1% and increasing. Many individuals with ASD do not possess a driver’s licence, indicating that access to public transport is crucial for their independence. However, at present, there is no research on the opinions of adults with ASD on public transport.AimTo identify the viewpoints of adults with ASD regarding the barriers and facilitators of public transport usage and their transportation preferences, and to contrast these against the viewpoints of neurotypical adults.MethodsQ method was used to identify the viewpoints of both participant groups on public transport. Participants consisted of 55 adults with a diagnosis of ASD and a contrast group of 57 neurotypical adults. Both groups completed a Q sort task which took place in either Perth or Melbourne, Australia.ResultsThe most prominent viewpoint indicated that both groups preferred to use public transport over driving and believed that it supported their independence. This viewpoint also indicated that both groups preferred to use electronic ticketing when using public transport. Interestingly, the second most prominent viewpoint indicated that both groups preferred to drive themselves by private car rather than use public transport.DiscussionIt appears that the viewpoints of adults with and without ASD regarding public transportation were largely similar. However, questions arose about whether the preference for public transport in the ASD group may be more a result of difficulties obtaining a driving licence than a deliberate choice. The only barrier specified by adults with ASD related to crowding on public transport. Safety and convenience in relation to location and timing of services were barriers reported by neurotypical adults.     

基质金属蛋白酶-9和白介素-18在冠心病及糖尿病大血管并发症中的作用

目的探讨基质金属蛋白酶-9(MMP-9)和白介素-18(IL-18)在冠心病及糖尿病大血管并发症中所起的作用。方法选择住院患者181例,分为冠心病组(n=50)、冠心病+糖尿病组(n=50)、糖尿病组(n=50),并设对照组(n=31)。冠心病组、冠心病+糖尿病组随访急性冠脉综合征(ACS)的发生情况,并检测IL-18、MMP-9含量;糖尿病组随访颈部血管内-中膜厚度(IMT),分别于6个月、12个月采血检测IL-18、MMP-9含量。结果 ACS发生后血清IL-18、MMP-9水平显著升高;MMP-9是ACS的独立危险因素(OR=5.19,P=0.014,95%CI 1.396¹9.298),IL-18与ACS的发生关系不明显;MMP-9浓度变化与IMT增量呈正相关(r=0.472,P=0.011,95%CI 0.00819.298),IL-18与ACS的发生关系不明显;MMP-9浓度变化与IMT增量呈正相关(r=0.472,P=0.011,95%CI 0.008⁰.095)。结论 MMP-9参与ACS及糖尿病大血管并发症的发病过程,其含量变化对ACS、糖尿病大血管并发症有一定的预测价值。     

多囊卵巢综合征患者抗苗勒氏管激素与干细胞因子的相关性

目的研究多囊卵巢综合征(PCOS)患者抗苗勒氏管激素(AMH)和干细胞因子(SCF)的表达情况及二者的相关性。方法选择年龄均小于35岁行体外受精与胚胎移植的PCOS患者102例,正常对照组90例,采用ELISA法检测血清和卵泡液中AMH和SCF的蛋白水平,分离并培养颗粒细胞;采用Real-time PCR检测PCOS患者颗粒细胞中AMH和SCF mRNA的相对含量,并对其进行相关性分析。结果①PCOS患者血清、卵泡液及颗粒细胞中AMH的相对表达较正常对照组明显升高,而SCF较对照组显著降低(P<0.01);②PCOS患者血清、卵泡液及颗粒细胞中AMH与SCF的表达均呈极为显著负相关(P<0.01)。结论多囊卵巢综合征患者血清、卵泡液及颗粒细胞中AMH与SCF的表达呈显著负相关。     

Klotho蛋白与心力衰竭患者肾脏损害的相关性

目的 研究klotho蛋白与心力衰竭患者肾脏损害的相关性.方法 收集我院2009年3月至2010年12月间住院的115例心力衰竭患者血样本,采用ELISA方法检测klotho蛋白,分析不同心力衰竭的程度、不同血肌酐(Cr)水平与klotho蛋白之间的关系.结果 心力衰竭患者NYHA Ⅰ～Ⅱ级、Ⅲ级及Ⅳ级血清klotho...     

一脆性X综合征家系的基因诊断及其动态突变

本文报告了使用探针pX6对一脆性X家系进行了分子杂交分析研究。结果表明:女性携带者的（CGG）的较小扩增遗传到子代出现（CGG）重复序列大量扩增,伴随CpG岛甲基化,从而导致FMR-1基因失活。     

肠易激综合征模型大鼠的大脑和消化道中5-HT的表达与病理作用

目的检测腹泻型肠易激综合征(D-IBS)和便秘型肠易激综合征(C-IBS)模型大鼠大脑及肠道组织中的5-HT分泌表达的差异,探索5-HT在IBS发病中的作用。方法采用冰醋酸加束缚应激的方法造成D-IBS动物模型,冰水灌胃法造成C-IBS动物模型,并鉴定模型。用免疫组化方法检测各组大鼠海马、下丘脑、空肠、回肠、近端结肠、远端结肠组织中5-HT阳性细胞并进行分析。结果IBS模型经鉴定符合其病理特征。结肠组织分析显示各组大鼠均无明显炎性表现。D-IBS组海马、下丘脑、空肠、回肠、近端结肠、远端结肠5-HT阳性细胞数显著多于对照组(P<0.05),并且空肠、回肠阳性细胞数也多于C-IBS组(P<0.05);C-IBS组海马、下丘脑、回肠、近端结肠、远端结肠阳性细胞显著多于对照组(P<0.05)。结论IBS模型大鼠大脑及肠道中5-HT的增加与IBS发病相关,5-HT在IBS不同亚型发病机制中的作用可能与其不同受体在大脑和消化道的表达分布差异有一定的关系。     

5羟色胺受体可塑性在大鼠肠易激综合征中的作用

目的探讨5羟色胺(5-HT)以及5-HT3、5-HT4受体可塑性在肠易激综合征(IBS)不同亚型发病中的作用。方法成年雄性SD大鼠27只,随机分为IBS合并腹泻组(IBS-D)I、BS合并便秘组(IBS-C)和空白对照组。分别采用乙酸灌肠、冰水灌胃的方法制备IBS-D和IBS-C大鼠模型。用5-HT以及5-HT3、5-HT4受体抗体的免疫组化方法检测各组大鼠肌间神经丛的5-HT及其受体数量的变化。结果①大鼠肌间神经丛5-HT阳性肠神经元数目IBS-D组(4.37±0.88)显著高于对照组(2.99±0.41,P<0.01),而IBS-C组(3.04±0.35)和对照组相比无差异(P>0.05);②大鼠肌间神经丛5-HT3受体免疫组化染色的灰度值各组间差异无统计学意义(F=0.082,P=0.922);③大鼠肌间神经丛5-TH4受体免疫组化染色的灰度值IBS-D组(141.98±6.96)显著低于对照组(159.90±2.55,P<0.01),而IBS-C组(155.62±2.03)与对照组差异无统计学意义(P>0.05)。结论大鼠肠肌间神经元内的5-HT含量及5-HT4受体数量的显著增多可能与IBS-D大鼠的发病相关。     

The clinical role of increase of serum matrix metalloproteinase-8 concentration in patients with acute coronary syndrome

Objective To study the clinical role of the variation of serum matrix metalloproteinase-8 (MMP-8)concentration in patients with acute coronary syndrome (ACS). Methods ELISA method was adopted to detect serum MMP-8 concentration and to observe concentration's differences and features among 80 selected ACS cases (43 acute myocardial infarction and 37 unstable angina pectoris), 43 stable angina pectoris (SAP) cases and 37 control cases.And meanwhile the atherosclerosis risk factors of each case, such as age, sex, hypertension, body mass index, smoking,family history, diabetes, and hyperlipidemia were collected and analyzed as a whole. Results First, serum MMP-8 concentration reached the highest point in ACS, and there was significant difference between SAP and control groups (P<0.01). Second, sermn MMP-8 in AMI was much higher than that in UAP with significant difference (P<0.01). There was no difference between UAP and SAP groups (P>0.05). Third, Logistic regression analysis revealed that serum MMP-8 concentration might be the indicator of ACS (B=4.493, P=0.000), particularly, that of AMI (B=9.961,P=0.000). Fourth, linear correlation and linear regression analysis found that only neutrophil was likely to influence serum MMP-8 concentration (r=0.274, P=0.001). Fifth, in the diagnosis of ACS, the area under ROC curve of MMP-8 was 0.785, the sensitivity and specificity were 68.6% and 76.5%, respectively. Conclusion ① Serum MMP-8 concentration has close relationship with the occurrence of ACS, particularly with AMI;② Serum MMP-8 concentration may be one of the predicting indicators of ACS and particularly of AMI;③ Neutrophil may be correlated with serum MMP-8 concentration;④ MMP-8 is of somewhat valuable in diagnosing ACS.