重组人单链白细胞介素12融合基因(rhscIL-12)的构建和真核表达

克隆人 IL - 1 2 ( h IL - 1 2 ) p40和 p35亚单位 c DNA,构建人单链 IL- 1 2 ( rhsc IL- 1 2 )融合基因 ,并在哺乳动物细胞中进行表达。方法　从经 PDBu刺激的 EBV转化的人 B淋巴母细胞株 NC37中提取 m RNA,经 RT- PCR分别获得 h IL- 1 2 p40和 p35亚单位编码序列的 c DNA,运用重组 PCR技术将两段基因通过一疏水性多肽接头 ( Gly4 Ser) 3 DNA序列进行体外基因重组 ,构建 rhsc IL- 1 2融合基因 ,将其插入 pc DNA3.1 ( + )真核表达载体 ,经脂质体转染 COS7细胞进行表达 ,Western blot进行分析。结果　所克隆的 h IL- 1 2 p40、p35c DNA序列和构建的 rhsc IL - 1 2融合基因 DNA序列均经测序得以证实 ,融合基因可在 COS7中表达其产物 rhsc IL- 1 2融合蛋白 ,其分子量为 70 KD,可与鼠抗人 IL - 1 2 p40 /p70单克隆抗体特异性结合。结论　本研究结果为进一步探讨 rhsc IL- 1 2融合蛋白的生物学活性和特性奠定了基础 ,也为 rhsc IL- 1 2融合基因在原核细胞中的表达提供了可能性     

THE INVESTIGATION OF DIFFERENT POPULATION＇S TRANSFUSION-TRANSMITTED VIRUS-DNA IN SHAANXI PROVINCE

Objective To investigate transfusion transmitted virus (TTV) infection among population of different groups in Shaanxi Province. Methods A nested polymerase chain reaction (PCR) with primers from ORF1 of TTV genome was established to detect TTV-DNA in serum of the patients. Results TTV-DAN was detected in the sera of 3 of 50 cases of general population(6％), 2 of 30 cases of vocational blood donors(6.7%),21 of 97 cases with Type B hepatitis (21. 6％), 9 of 35 cases of Type C hepatitis (25. 7％),and 23 of 40 cases with non-A～ non-G hepatitis (57.5 ％ ). Conclusion There is TTV infection among general population in Shaanxi Province. TTV may be an impor- tant agent to cause non-A～non-G hepatitis. And the patients with HBV or HCV can have overlapping TTV infection.     

RAPID MOLECULAR DETECTION OF METHICILLIN-RESISTANT S. AUREUS IN THE INTENSIVE CARE UNIT

Staphylococcus aureus is found in the nose andon the skin of a variety of healthy individuals,andis an opportunistpathogen in patients with loweredhost resistance.The isoxazolylpenicillin (e.g.me-thicillin,cloxacillin,flucloxacillin) have been themainstay of treatment for infection over2 5years.But emergence of antibiotic resistance(MRSA) is aproblem in hospitals due to transmission of epi-demic MRSA strains with antibiotic resistance.Ac-quisition of MRSA continues to be of major con-cern…     

非均匀条件下的船舶锚链静力特性快速计算

为探索锚泊船偏荡时船与锚链相互作用问题,开展了非均匀条件下锚链静力特性的快速计算研究。采用分段外推-校正方法计算了锚链张力、顶端倾角、悬垂线形状及长度。通过寻找锚链悬垂线最低点位置以及预估顶端倾角的方法来增加求解速度,实现了快速运算。     

中海工业推行修船生产精益化以实现修船转模

文章根据生产控制管理理论,结合中海工业的修船实际,分析了修船企业应对金融危机的重要对策是推进生产精益化并且以计划管理为抓手,进而具体展开对修船转模的研究,为形成中海修船的核心竞争优势作出有益探讨。     

强化策划和生产计划控制,实行规范有序维修舰船

文章阐述了对修船产品实现过程要进行策划控制和生产计划控制,以形成规范有序修船。分别介绍了两种控制的主要内容,并列出了强化策划控制和生产计划控制管理的主要措施。     

Competition model of cruise home ports based on the cruise supply chain – based on China cruise market

This paper addresses issues of cruise home port competition based on the cruise supply chain. A pricing model of the cruise market is developed considering the competitive cooperation behaviors of the participants in the cruise supply chain. Furthermore, a model of cruise home port competition is developed to investigate the impacts of subsidy participants in the cruise supply chain on cruise home ports. The results indicate that subsidy policies change the market equilibrium, promote the upstream and downstream integration of cruise supply chain, and enhance cruise home port competitiveness. Subsidies to cruise line increase the payoffs of cruise supply chain and ports’ profit. Subsidies to travel agency decrease passenger costs and improve all ports’ profits. For the long-term development of cruise home port, the consequents can be used as policy suggestions.     

中职汽车维修专业教学体系革新策略研究 ——基于产教融合背景

本文提出了中职汽车维修专业教学体系革新策略,首先分析了产教相融合的元素及对提升学生专业技能的效果,其次对产教融合背景下汽车维修专业教学体系革新建设路径予以了细化探析,分别从教学体系思路框架革新、专业教学体系革新及明确建设目标等三大方面深入论述.最后对教学体系革新策略开展后产生的预期结果予以了分项明确.旨在为社会与汽车行...     

系统与造船区域生产管理

现代造船模式对造船的发展具有积极的作用,造船区域生产管理有助于现代造船模式"按区域组织生产"的实施,文章对比进行了较详细的分析研究.     

浮式桥式集装箱起重机船岸作业系统效率分析

为完善新近提出的适应斜坡式集装箱码头的浮式桥式集装箱起重机船岸作业系统设想,开发了相 应的计算机动画模拟模型,并应用其辅助系统方案的选择和参数的确定。本文介绍该模拟模型及应用其研究分析 浮式桥式集装箱起重机船岸作业系统作业效率问题的方法和结论。