AQP4在COPD支气管黏膜的表达及其与患者肺功能、气道黏膜炎症的相关性

目的通过免疫组化法检测支气管黏膜中水通道蛋白-4(aquaporin-4,AQP4)的表达,观察正常组和慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)组支气管黏膜中AQP4表达与气道黏膜炎症及肺功能的关系,明确AQP4在COPD发生、发展中的作用,为COPD的防治开辟新途径。方法选择正常人和COPD患者各30例,测试肺功能,并行支气管镜检查,取得支气管黏膜标本,用免疫组化法检测AQP4表达,HE染色观察支气管黏膜炎症细胞浸润程度。结果COPD组较对照组AQP4表达减弱有极显著差异(P<0.001)。AQP4表达和FEV1/FVC、FEV1/pred%呈正相关(P<0.001),与支气管黏膜炎症严重程度呈负相关(P<0.001)。结论COPD患者AQP4表达减少可能是其肺功能下降及气道炎症加重的原因之一。临床上可以通过改善AQP4治疗COPD。     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.     

Study on T lymphocyte subsets and NK cells in patients with Graves＇ disease combined with type 2 diabetes

Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves' disease (GD)and Graves' disease combined with type 2 diabetes mellitus (GD/T2DM). Methods Fifteen cases of GD/ T2DM were selected from our hospital from November 2001 to November 2004. Before and after therapy thyroid function, thyroglobulin antibody (TGA), thyroid microsomal antibody (TMA) and blood glucose level were measured, and T lymphocyte subsets (CD3, CD4, CD8, CD4/CD8) and NK cells (CD56) were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry, respectively. At the same time, comparison was made with simple GD (15 cases), T2DM (15 cases) and healthy control (20 cases). Results Before therapy, CD4/CD8, CD4 and NK cells in GD/T2DM were less than normal, and there was no significant difference in comparison with simple GD (P<0.05). In T2DM group, only CD4/CD8 and CD4 were less than those of healthy controls (P<0.05). When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups, various indexes recovered, which were more obvious in simple GD. Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM, which is more significant than that of simple GD or T2DM. The recovery of thyroid function and immune abnormality is not consistent, and the recovery of GD is more significant than that of GD/T2DM.     

丘陵地区道路桥梁设计探讨

结合我国丘陵地区道路特点,介绍丘陵地区桥梁设计中跨径的选择原则,墩、台、基础和结构体系宜采用的形式以及桥梁和路基之间的关系,并对丘陵地区道路桥梁设计中存在的问题和相应措施进行探讨,给丘陵地区道路桥梁设计提供参考。     

基于现代物流理论的公路快速货物运输组织

公路快速货运系统作为一种先进的运输组织形式,能够提供快速、安全、准确和“门到门”的运输服务。从第三方物流、公路货物运输与现代物流的关系等理论出发,在分析公路快速货运系统组成的基础上,对其货物运输组织形式进行研究,提出公路快速货物运输组织的发展思路。     

城市交通公平性分析及对策

城市道路的交通公平性有利于建设和谐、公平的城市,在交通公平的定义以及它的三层含义的基础上,陈述了交通公平在时间、空间和内容上的内涵。分析城市道路交通公平的影响因素及其判断标准,阐述了快速公交符合城市道路交通公平的判断标准是它有利于大多数人的出行,满足弱势群体的出行要求,并应具有低成本、高质量的优点。最后,以北京市BRT的实践表明快速公交是提高城市交通公平性的有效措施。     

基于沥青路面开裂病害的路况质量评价标准的探讨

针对沥青路面裂缝对路面寿命造成的严重影响,对沥青路面养护质量评定方法进行探讨,提出沥青路面破损率评定的新方法,对节约资源、节约资金、提高道路养护质量、延长沥青路面寿命有着重要的现实意义。     

高等级公路膨胀土路基病害与处治措施

膨胀土是一种对工程建设有特殊危害的土。针对膨胀土公路路基的破坏形式,分析膨胀土路基破坏的原因,并结合某高速公路膨胀土路基病害探讨膨胀土路基的处治措施,可为此类路基处治提供参考。     

作业成本法在路政管理中的应用研究

基于作业成本法在路政管理系统成本测算中的研究,并结合江苏省路政管理作业模式的改进,重点对作业成本法在路政管理成本测算和控制中的应用流程进行分析,研究成果使路政管理部门能够更为准确地控制和把握路政管理各项作业活动中的各种资源,从而有利于提高路政管理工作的效率。     

不同功能城市道路的车辆横向分布特征

针对城市道路路面设计中车道横向分布系数的使用情况,对交通性干道、综合性干道和商业性干遭这三种类剐的干道分别进行交通调查,并提出四车道、六车道城市道路的车道分布系数,有利于更科学合理地进行城市道路的路面设计。