生存素和CD44V6蛋白在胃癌中的表达及其相关性

目的探讨生存素（survivin）和CD44v6蛋白在胃癌中的表达及意义。方珐应用免疫组化技术检测120例胃癌（GC）、30例异型增生（GED）和20例正常胃黏膜组织（NGM）中survivin和CD44v6蛋白表达，并结合肿瘤的病理学行为和临床随访资料进行分析。结杲在胃癌组织中，survivin和CD44v6阳性率分别为75．8％和81．7％，均显著高于GED和NGM（P〈O．05）；survivin和CD44v6表达与GC浆膜浸润、淋巴结转移和患者预后密切相关（P〈0．05）。结论Survivin和CD44v6表达与GC发生、转移和患者生存期有关，检测survivin和CD44v6蛋白表达可作为预测胃癌患者预后的参考指标。     

重组人突变型471TNF-α及野生型TNF-α的表达及活性初步鉴定

目的　采用原核表达系统 ,表达人突变型 471TNF α蛋白 ,并检测其生物学活性。方法　采用PCR技术扩增突变型 471TNF α及野生型TNF αcDNA片段 ,克隆入中间载体pUCm T中 ,并测序证实。以限制性内切酶切下目的片段 ,克隆入pBV2 2 0中 ,转入大肠杆菌DH5α ,温度诱导表达野生型及突变型 471TNF α蛋白。初步纯化蛋白后 ,采用MTT法评估两者对L92 9细胞的杀伤作用。结果　①获得了测序正确的野生型TNF α及突变型 471TNF α的cDNA克隆 ;②构建了正常人野生型TNF α及突变型 471TNF α重组表达质粒 ;③经温度诱导 ,TNF α及突变型 471TNF α蛋白均获得了表达 ;④初步的生物学活性研究表明 ,突变型 471TNF α蛋白的杀伤作用显著高于野生型。结论　突变型 471TNF α的生物学活性优于野生型TNF α ,为进一步开展TNF α的基础及临床研究奠定了基础     

Nonlinear programming methods based on closed-form expressions for optimal train control

This paper proposes a novel approach to solve the complex optimal train control problems that so far cannot be perfectly tackled by the existing methods, including the optimal control of a fleet of interacting trains, and the optimal train control involving scheduling. By dividing the track into subsections with constant speed limit and constant gradient, and assuming the train’s running resistance to be a quadratic function of speed, two different methods are proposed to solve the problems of interest. The first method assumes an operation sequence of maximum traction – speedholding – coasting – maximum braking on each subsection of the track. To maintain the mathematical tractability, the maximum tractive and maximum braking functions are restricted to be decreasing and piecewise-quadratic, based on which the terminal speed, travel distance and energy consumption of each operation can be calculated in a closed-form, given the initial speed and time duration of that operation. With these closed-form expressions, the optimal train control problem is formulated and solved as a nonlinear programming problem. To allow more flexible forms of maximum tractive and maximum braking forces, the second method applies a constant force on each subsection. Performance of these two methods is compared through a case study of the classic single-train control on a single journey. The proposed methods are further utilised to formulate more complex optimal train control problems, including scheduling a subway line while taking train control into account, and simultaneously optimising the control of a leader-follower train pair under fixed- and moving-block signalling systems.     

Fourier NUBS method to express ship hull form

This article presents a method of numerical expression to draw ship hull forms. Recent developments in research into ship hull optimization need a method to express ship hull form as precisely as possible with a small number of design parameters. This method is based on a combination of the Fourier–sine series expansion and nonuniform B-spline (NUBS) interpolation. The merit of the combination is that it removes the wiggles which are often found when generating a rectangular-type curve, such as the midship section of tanker ship, with a simple Fourier series expansion. Here, the procedure is explained, and then a tanker ship hull is generated. Through the discussion, we show the effectiveness of the method.     

一种基于幂级数展开和最小二乘法的高速公路稳态速度-密度平衡关系式的辨识算法

提出了一种新的辨识Markos Papageorgiou提出的高速公路交通流模型中稳态速度-密度平衡关系式的算法.该算法分为两个步骤,第1步,利用幂级数的原理,将该关系式由非线性模型转换成线性模型;第2步,采用最小二乘法辨识线性模型,并在此基础上获得非线性模型的所有参数,从而达到辨识稳态速度-密度平衡关系式的目的.理论分析和仿真结果均表明,与传统的非线性辨识算法相比,该算法大大提高了模型辨识的运算速度,同时可以实现任意的辨识精度.     

高速公路入口匝道多维可调模糊控制器设计

鉴于入口匝道多维模糊控制器的控制规则库会随着人们控制方面经验的增加而变得愈加复杂,从而造成规则之间的冗余性、兼容性问题.因此,给出入口匝道多维模糊控制器的解析描述,不仅有效地解决了多维模糊控制器控制规则之间的冗余性、兼容性问题,而且通过在不同状态下引入不同加权因子实现了控制器的自调整,使所设计的模糊控制器更能适应实际的高速公路交通需求.最后通过MATLAB仿真得到的结果表明,控制效果令人满意.     

搅拌筒螺旋线平面展开方程及基于UG的参数化表达式

推导出搅拌运输车搅拌筒对数螺旋线平面展开方程,用UG的参数表达式来实现螺旋线和在平面上展开曲线的生成。     

汽车交流发电机畸变电动势的理论分析

基于交流发电机畸变电动势的形成过程，推导了汽车交流发电机实际工作的畸变电动势表达式。     

饰胶蛋白(Decorin)基因的cDNA克隆与表达

目的　通过基因克隆技术获得饰胶蛋白基因cDNA克隆并表达。为研究饰胶蛋白的生物学功能及应用打基础。方法　应用PCR技术从T细胞cDNA文库获得饰胶蛋白全长基因cDNA片段 ,并克隆到pUC1 9载体中 ,采用Sanger双脱氧末端终止法测定全部cDNA序列 ,将测序正确的饰胶蛋白cDNA序列插入pGEX 4T 1表达载体中 ,经BamHI和EcoRI双酶切后 1 2 %凝胶电泳鉴定 ,IPTG诱导表达产物经SDS PAGE分析。结果　克隆的饰胶蛋白cDNA序列与GenBank中AF1 3830 0记载的序列完全一致 ,所表达的融合蛋白质分子量与理论计算值(65 6KD)也一致并为可溶性蛋白。结论　本研究成功地克隆了饰胶蛋白基因和表达了GST Decorin融合蛋白