机车蓄电池检测技术探讨与应用

针对目前铁路机车蓄电池对机车质量造成的影响,通过对蓄电池的几种常规检测方法:放电容量检测、电解液密度检测及其他检测方法等的分析和比较,指出各种检测方法的优缺点,提出对蓄电池采用动态监测手段的必要性。根据检测到数据来判断电池质量的好坏,从而提高了蓄电池的使用寿命,减轻工人的劳动强度,具有很好的经济效益。     

硒对克山病区粮饲养大鼠心肌细胞的影响——超微结构及膜通透性的改变

本实验用克山病区低硒粮饲养大鼠,造成动物低硒状态,同时增设补硒组和常备饲料组大鼠作为对照,饲养2个月后,应用示踪剂分别观察其心肌细胞超微结构及膜通透性。结果低硒组大鼠部分心肌细胞膜不完整,镧颗粒进入到细胞内。钌红使质膜染色呈中等电子密度的不均匀窄带,并经质膜破损处进入心肌细胞内。补硒组大鼠变化轻微。提示克山病区粮可引起大鼠早期心肌细胞超微结构及膜通透性的改变,补硒可使之缓解或改善。     

由近似边界进行边缘改善的几种珠子链算法的分析和比较

本文提出了由近似边界确定物体边界的简易珠子链算法,该算法较珠子链算法和改进珠子链算法简单。试验证明:加上约束条件的简易珠子链算法比不加约束条件的改进珠子链算法算法精确。     

高层建筑转换层结构设计与探讨

本文首先详细介绍了高层建筑转换层的概念、结构特性和设计原则,同时根据转换层的功能特点和要求,介绍其设计要点,包括设计战略、构件选择、配筋设计和轴压比控制要求等重点探讨了转换层结构设计的要点,可供类似工程设计参考。     

经鼻腔、蝶窦至海绵窦手术入路的解剖学基础

1987年,M.Loyo 首次报道经鼻腔、蝶窦而入海绵窦,处理海绵窦内血管疾患的新手术入路.本文作者用30个(60侧)尸头及51个干颅骨(102侧)做了蝶窦的外科解剖观察,除为经蝶窦而达垂体的手术入路提供了数据外,本文侧重论证经蝶窦而达海绵窦手术入路的解剖基础及优点。     

Distribution of nitric oxide synthase, nerve growth factor receptor and interstitial cells of Cajal in hirschsprung's disease and its significance

Objective To investigate the distribution of nitric oxide synthase (NOS), nerve growth factor receptor (NGFR) and interstitial cells of Caial (ICCs) in Hirschsprung's disease (HD). Methods The distribution of NOS,NGFR and ICCs was studied by using NADPH diaphoruse histochemistry, immanohistochemistry with a monoclonal antibody to human NGFR and the specific polycloual antibody against c-kit in 8 normal controls and 10 cases of HD.Results NOS and NGFR were abundantly present in the myenteric plexus and in the nerve fibers of musculature. ICCs were intensively distributed in the surface of circular musculature and around the myenteric plexus to form a network in normal control colon. In contrast, NOS and NGFR were scarce or absent in the myenteric plexus and in the nerve fibers of musculature, while the hypertrophic nerve trunks were NGFR positive, ICCs were scarcely distributed and the network was disrupted in the aganglionic colon in HD. Conclusion These findings suggest the Involvement of NOS,NGFR and ICCs in the patbophysiology of HD.     

Methylation status of the interferon-gamma gene promoter in chronic hepatitis B

Objective To evaluate the methylation status at CpG site -55 in the interferon-gamma (IFN-7) gene promoter and its effect on IFN-7 expression in chronic hepatitis B. Method The authors recruited 30 patients with UBeAg-positive chronic hepatitis B (CHB), 30 HBeAg-negative CHB patients, and 30 healthy blood donors. Pyrosequeneing was used to determine the methylation status at CpG site -55 in the IFN-γ gene promoter following bisulfite treatment of DNA in peripheral blood mononuclear cells (PBMCs). The expression of IFN-γ was analyzed by real-time RT-PCR and ELISA. HBV DNA in PBMCs was detected by nested PCR. Results The methylation level at CpG site -55 in the IFN-γ gene promoter was significantly increased, resulting in subsequent down-regulation of the expression of this cytoldne in CHB. The methylation level at CpG site -55 was significantly higher in HBeAg-positive patients than in HBeAg-negative ones (P<0.01) and was also significantly higher in PBMCs from HBV DNA-positive patients than from HBV DNA-negative ones (P<0.01) ; the methylation level at CpG site -55 was positively correlated with the amount of HBV DNA in serum (P<0.01). Oonclusion IFN-γ gene expression appears to be regulated by methylation of the IFN-γ gene promoter in CHB; the methylation level at CpG site -55 is associated with HBV infection.     

斜拉桥索无损检测信号的滤波处理方法

针对在役斜拉桥索只能进行无损检测的特点,设计了基于漏磁检测的缆索缺陷检测系统。实测结果表明,缺陷产生的漏磁信号往往附有大量的噪声信号,导致信号特征不显著。为此,应用信号平滑、小波包差分超限滤波和BP神经网络模式识别等信号分析方法,对采集信息进行滤波处理,以获取比较显著的特征量化信号。仿真结果表明,经过滤波处理后的漏磁信号特征明显,为桥梁斜拉索的断丝缺陷检测和分析提供了可靠依据。     

预防富水铁路隧道道床翻浆冒泥病害的设计施工措施探讨

针对富水铁路隧道内道床翻浆冒泥病害,以现行铁路隧道通用图为基础,结合富水隧道地下水水量大且具承压性的特点,对通用图中洞内防排水设施功能分析后提出改进方向,形成以降水压、消隐患、排渗水的富水隧道洞内防排水设计方案总体思路;并提出了富水隧道洞内增设降水井,调整双线隧道侧沟位置并加深,优化中心水沟井壁设计,道床板下增设渗水盲管等具体防排水改进措施。针对隧底存在虚碴层问题,提出采用水平光面爆破开挖,增加人工清底工序,按隐蔽工程验收及无损探测检查等具体施工质量控制措施。该设计施工预防措施经工程验证是有效、可行的。     

PPAR-γ激活对血管平滑肌细胞外基质释放及结缔组织生长因子表达的影响

目的观察PPAR-γ激活对血管紧张素Ⅱ诱导的体外培养条件下大鼠血管平滑肌(VSMCs)的细胞外基质(ECM)释放及结缔组织生长因子(CTGF)表达的影响。方法原代培养大鼠VSMCs,用不同浓度PPAR-γ激动剂rosiglitzone和/或抑制剂GW9662、BADGE预处理后,加入0.1μmol/L血管紧张素Ⅱ(AngⅡ)刺激24h。比色法检测各组细胞培养液中羟脯氨酸含量,实时荧光定量RT-PCR方法检测各组三型胶原(ColⅢ)、层粘连蛋白(FN)及CTGFmRNA表达,Western blotting检测各组CTGF、PPAR-γ蛋白表达情况,基于ELISA的DNA-binding检测各组PPAR-γ活性。结果 AngⅡ可增加VSMCs PPAR-γ表达,但明显抑制其活性(P<0.05,vs.Control),PPAR-γ激动剂rosiglitazone可显著增加PPAR-γ蛋白表达及活化(P<0.05,vs.AngⅡ)。rosiglitazone可降低细胞培养液中羟脯氨酸含量,下调VSMCs中ColⅢ、FN、CTGF mRNA表达,抑制CTGF蛋白表达,而其他PPAR-γ激动剂(pioglitazone、15-d-PGJ2)均有相似作用,PPAR-γ拮抗剂GW9662及BADGE可拮抗这一作用。结论在VSMCs中,PPAR-γ激活可抑制AngⅡ诱导的CTGF表达,同时抑制ECM沉积。提示PPAR-γ可能在血管纤维化中起重要作用。