HAIR CELL-LIKE CELL GENERATION INDUCED BY NATURE CULTURE OF ADULT RAT AUDITORY EPITHELIUM

Objective To establish adult rat auditory epithelial cell culture and try to find precursor cells of auditory hair cells in vitro. Methods With refinement of culture media and techniques, cochlear sensory epithelial cells of adult rat were cultured. Immunocytochemistry and Bromodeoxyuridine （BrdU） labeling were used to detect properties and mitotic status of cultured cells. Results The cultured auditory epithelial cells showed a large, flat epithelial morphotype and expressed F-actin and cytokeratin, a subset of cells generated from auditory epithelium were labeled by calretinin, a specific marker of early hair cell. Conclusion Adult rat auditory epithelium can be induced to generate hair cell-like cells by nature culture, this phenomenon suggests that progenitor cells may exist in rat cochlea and they may give birth to new hair cells. Whether these progenitor cells are tissue specific stem cells is still need more study.     

醛固酮及螺内酯对肝星状细胞中TIMP-1和MMP-2mRNA表达的影响

目的观察醛固酮及螺内酯对活化的肝星状细胞(HSC-T6)作用后不同时间段MMP-2和TI MP-1 mRNA的表达情况。方法采用RT-PCR的方法测定HSC-T6中MMP-2、TI MP-1 mRNA的表达。结果活化的HSC可表达MMP-2及TI MP-1的mRNA。醛固酮组MMP-2、TI MP-1的mRNA在48、72 h表达均高于对照组,在作用24、48、72 h时MMP-2、TI MP-1的mRNA的表达随时间递增。而螺内酯组则相反。醛固酮加螺内酯组与单加醛固酮组相比MMP-2、TI MP-1的mRNA表达下降。结论醛固酮可能通过促使MMP-2及TI MP-1 mRNA的表达促纤维化,螺内酯可抑制这一作用。     

Establishment of novel embryonic stem (ES) cell lines from OG2/rtTA blastocysts

Embryonic stem (ES) cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation....     

硒与锌对培养人胚心肌细胞~3H-TdR参入DNA的影响

本文应用不同浓度的硒(0.50、0.75、1.00μg/ml)和锌(0.10、0.50、1.00μg/ml)分别加入心肌细胞培养液中,观察两者对~3H-TdR 对 DNA 参入率的影响.实验结果显示,加入 Se 0.50μg/ml 及 Zn 0.50～1.00μg/ml,能增加~3H-TdR 的参入率。     

船舶纵向气囊下水气囊滚动阻力分析

船舶气囊下水工艺现已得到广泛应用,而气囊滚动阻力是影响该类船台坡度设计的主要因素。通过构建气囊滚动阻力计算模型与对影响滚动阻力的主要因素的分析,提出了该类船台坡度的计算方法,以供设计参考。     

人宫颈癌细胞凋亡与Bcl-2表达的研究

目的　探明 Bcl- 2在宫颈癌细胞凋亡过程中的作用。方法　采用 60 Co和顺铂处理体外培养的 3株人宫颈癌细胞株 Hela、Si Ha、RJC,2 4 h后检测细胞凋亡和 Bcl- 2。结果　发现放射线 60Co和顺铂均可以诱发宫颈癌细胞凋亡 ;它们都表达 Bcl- 2 ,其表达率随着细胞凋亡的出现而下降 ;两种因素联合应用后诱导细胞凋亡和降低 Bcl- 2表达的作用增强。结论　 Bcl- 2的表达可能参与了宫颈癌细胞凋亡的调节过程     

正常人PBMC对重组人_γIL_4促增殖作用的应答

本实验比较了新鲜分离的正常人外周血单个核细胞(PBMC)和佛波脂类促癌剂TPA活化的PBMC对重组人白细胞介素4(γIL_4)促增殖作用的应答。发现γIL_4可剂量依赖性地促进新鲜分离的PBMC增殖,但其程度太小不足以灵敏反应γIL_4量的变化,TPA可激活PBMC,从而提高PBMC中活化细胞比例,活化的PBMC对γIL_4刺激的应答增强,细胞增殖与rIL_4浓度之间存在较明显的正相关性。因此TPA活化的PBMC的增殖反应可作为一种较为简便,灵敏的γIL_4定量检测方法。     

胃癌癌旁及非癌胃粘膜内分泌细胞的研究

用胃泌素及生长抑素兔抗人抗体，ABC疫组化法，对55例手术之胃癌癌旁粘膜及67例非癌胃粘膜中的G细胞及D细胞进行了定位计数，观察其数值变化，初步探讨它们在胃癌发生发展中的作用。结果显示癌旁窦粘膜中G细胞及D细胞每平方毫米的平均数显著低于正常及CSG窦粘膜（P＜0．01及P＜0．05）。提示当窦粘膜内G细胞及D细胞的数量减少至正常值的1／3左右时，胃粘膜上皮严重失去激素的营养调节作用，容易接受内外源性致癌物的作用而发生癌变。     

扶正升白胶囊升高白细胞的药效学研究

采用纯苯、环磷酰胺、60Co照射制成小鼠白细胞减少病理模型，观察扶正升白胶囊对病理小鼠周围血白细胞数的影响。结果证明：该方对小鼠白细胞减少有显著的治疗及预防作用，并能升高血红蛋白及血小板。     

THE ULTRASTRUCTURAL OBSERVATION OF TARGET CELLS ATTACKED BY HUMAN LAK

In1982,Grimmetalfirstrepor=edthatanti-tumorlymph0kine-activatedkillercells(LAK)c0uldbegeneratedfromperipheralbloodmononuclearcells(PBMC)activatedbyinterleukin-2(IL-2)`l).Duet0theirhighertumor-kil1ingef-fect,LAKhavebeenusedinad0ptiveim-munotherapyforcancerpatientsandtheclinicalresultsarerathersatisfact0ry',,.ButthekillingmechanismofLAKisnotveryclearyet.Zychlinskyetal0bservedthem0rphol0gyoftargetcellsattackedbymurineLAKandfoundtargetcellsdiedvia.necrosi5andnpnpt0si5simultaneously"'.XuZhe…