地铁盾构机掘进实时姿态定向测量的研究

简明介绍了地铁建设中盾构机姿态定位测量方法,并结合南京地铁一号线某盾构区间隧道工程实际,着重分析了在对盾构推进过程中如何用棱镜法精确地确定盾构机的掘进方向和盾构机姿态问题,得出一些有益的结论,可供地铁隧道工程施工参考。     

深竖井定向测量实践

地下工程采用竖井作为施工通道，以增加开挖工作面，达到快速施工的目的，本文通过实例系统介绍竖井定向的多种测量方法。     

碰定向偏差自动修正的理论与实现技术

本文利用球谐函数解法结合地球磁场及我国海区的实际，给出了磁定向偏差的自动修正理论与实现技术，计算结果与国家测绘部门所提供的海图等资料符合甚好，可供水下定向之用。     

无定向导线及其严密平差在线路测量中的应用

由于公路等级的提高，对线路测量提出了更高的要求，本文讨论无定向导线测量及严密平差应用于线路测量的优点，并用实例证明它是一种高效，快速，经济的线路测量方法，有广泛的应用前景。     

集团装药定向爆破在拆除石拱桥上的应用

本文介绍了采用集团装药定向爆破的方法拆除石拱桥的应用。     

PRELIMINARY STUDY OF A NOVEL HUMAN PAPILLOMAVIRUS TYPE 16L1／E6-E7 CHIMERIC RECOMBINANT DNA VACCINE

Objective Preparations of HPV16 L1/E6 and L1/E7 prophylactic and therapeutic DNA vaccines. Methods The nucleotides within HPV16 E6 and E7 genes, which are responsible for viral transforming activity, were mutated by mage primer site-directed mutagenesis method. The correctly mutated E6 and E7 fragments were separately cloned into an eukaryotic expression vector pVAX1, together with HPV16 L1 gene, generating chimeric recombinants plasmids 1MpVAX1-L1E6, 2MpVAX1-L1E6, 1MpVAX1-L1E7, 2MpVAX1-L1E7 and 3MpVAX1-L1E7. CHO cells were transiently transfected with the individual DNA vaccines by calcium phosphate method. Target protein expressions in the extracts of the transfected cell lines were measured by ELISA and immunohistochemistry, with HPV16 L1 and E6 specific monoclonal antibodies. Results ELISA assays showed the P/N ratios in the cell extracts transfected with L1E6 and L1E7 plasmids were more than 2.1. Immunohistochemistry revealed brownish precipitant signal in cytoplasm and nuclei of the transfected cells. Conclusion Successful constructions of prophylactic and therapeutic DNA vaccine plasmids lay solid foundation for future animal experiment and clinical trial.     

隧道控制测量中竖井定向方法

结合隧道建筑中经常用开挖竖井增加工作面的实际情况,介绍了隧道控制测量中竖井定向常用的两种方法以及应注意的问题.