Protective effect of glutamine pretreatment on ischemia-reperfusion injury of spinal cord in rabbits

Objective To investigate the effect of glutamine (Gln) on the content of reduced glutathione hormone (GSH) and aminoglutaminic acid (Glu) of spinal cord following ischemia-reperfusion injury. Methods Totally 40 healthy adult male rabbits were randomly divided into five groups: sham-operation group (S group), ischemia-reperfusion injury group (I/R group), low-dose glutamine group (L Gln group), median-dose glutamine group (M Gln group) and high-dose glutamine group (H Gln group). After glutamine preconditioning, the model of spinal cord ischemia-reporfasion injury was established according to Zivin's method. The general status of animals was observed and the changes of Jacobs scoring were recorded in each group. Malondialdehydes (MDA), GSH, Glu and superoxide dismutase (SOD) activity in lumbar spinal cord tissues were determined using chemical colorimetry. The neuron number and deviation rate in spinal cord anterior horn were observed histopathologically. Results There was no significant difference between L Gin group and I/R group in behavior scoring, SOD activity, content of MDA and Glu, neuron number and deviation rate of spinal cord (P>0.05); however, there was a significant difference in GSH content of spinal cord (P<0.05). M Gln group and I/R group differed significantly (P<0.05) in behavior scoring, SOD activity, content of MDA, Glu, GSH, neuron number and deviation rate of spinal cord. Between H Gln group and M Gln group, there was no significant difference in behavior scoring, content of MDA and Glu, SOD activity, neuron number and aberration rate in spinal cord (P>0.05), whereas there was a significant difference in SOD activity and Giu content (P<0.05). Conclusion Pretreatment with medium-dose glutamine has a protective effect on spinal cord ischemia-reporfasion injury in rabbits, which may be related to the maintenance of GSH content, increase of SOD activity and reduction of MDA.     

Effect of ulinastatin on hepatic ischemia-reperfusion injury in rats

Objective To investigate the effect of ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats. Methods Totally 24 adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated control group (SO group), ischemia-reperfusion group (I/R group) and ulinastatin group (UTI group). Liver in I/R group underwent 1 h of reperfasion after 30 min of ischemia. In UTI group, UTI (2×104 U/kg) was administered to rats 30 min before modeling. The levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha (TNF-α) in serum were measured and the levels of nitric oxide and malondialdehyde in liver were determined. The histological changes of liver were observed. Results The levels of alanine aminotransferase, aspartate aminotransferase and TNF-α in serum were significantly increased in I/R group compared with those in UTI group (P<0.05). The levels of nitric oxide and malondialdehyde in liver were significantly higher in I/R group than in UTI group (P<0.05).Histological examination of liver indicated that the damages were more severe in I/R group than in UTI group.Conclusion UTI has the ability to inhibit the production of TNF-α and oxyradical, and ameliorate microcirculatory dysfunction in rats with hepatic ischemia-reperfasion injury.     

综合识别指标在桥梁结构损伤识别中的应用

提出一种应用振动模态频率变化值和静力位移变化值之比而建立的综合识别指标对桥梁损伤进行分析与评定.试验结果表明,应用该方法能够对损伤的位置和程度进行准确的定位和评估.既安全又方便,可为现在的桥梁检测提供参考.     

绿色的煤码头——广州港集团西基港务分公司狠抓环保工作

最近,笔者来到华南地区规模最大、自动化程度较高的煤炭装卸主力码头——广州港集团有限公司西基港务分公司（简称“西基港”）采访。刚踏进港区,便见港区内卸船机上的喷洒漏斗在不停作业;高如山坡的煤堆场周边,喷洒水枪实行360&#176;旋转式喷淋：皮带输送机两边装有整齐的防尘罩、挡风板,皮带机转接房、火车装卡塔台喷洒设备规范齐全;码头内污水处理池、水泵房一应俱备……     

钢管混凝土系杆拱桥损伤吊索系统内力重分布研究

以某下承式钢管混凝土系杆拱桥为背景,建立该桥的三维有限元模型,采用有效横截面积减小率模拟吊索损伤,通过仿真计算分析单根吊索和多根吊索发生不同程度损伤时,吊索系统静拉力重分布规律.结果表明,损伤吊索系统的静拉力重分布只在损伤吊索与其两侧相邻吊索间进行,多根吊索损伤引起吊索系统静拉力重分布近似等效于各单根损伤吊索引起静拉力重分布的线性叠加.该结论对于系杆拱桥吊索系统力学分析与评估具有较大的工程实用价值.     

基于自回归移动平均模型的梁桥损伤识别

桥梁结构的损伤识别是当前桥梁工程内十分活跃的研究领域,有着广泛的应用背景,与此相关的理论和技术也在不断的发展和成熟。基于自回归移动平均模型的研究方法以往都应用于结构的状态预测或系统识别,将其用于桥梁结构的损伤识别中还处于发展和完善阶段。拟结合桥梁结构的自身特点,对该方法在桥梁结构损伤识别中的应用做进一步的研究。     

基于车致振动的桥梁损伤识别

基于移动车辆引起的桥梁振动,采用模式识别的聚类分析方法对桥梁结构损伤定位及损伤程度估计进行了研究．应用车致振动理论计算不同预设损伤位置和损伤程度的桥梁结构在相同移动车辆作用下的动力响应,记录其在测试位置的响应,形成损伤模式库;将在测试位置实际测得的响应与损伤模式进行比较,并利用聚类分析的近邻法识别桥梁结构是否出现损伤、损伤位置及其程度．为直观有效地表示识别结果,分别以损伤位置和损伤程度为独立坐标轴,用等值线和响应云图的方式展示比较结果．以移动荷载匀速通过某简支梁为例,验证了该方法的有效性和鲁棒性．数佰模拟结果表明．该方法能够有效地估计桥梁结构的榀作付罟和榀作程摩．     

基于结构性能的PC连续刚构桥损伤分析

为探讨PC(预应力混凝土)连续刚构桥开裂及下挠的原因,提出了基于结构性能的损伤分析方法.该方法立足于结构性能的客观表象,把对可能造成损伤的诸多因素对结构性能水平的影响分析作为主要手段;通过对大量理论计算与试验数据的对比分析,以基于实桥检测和荷载试验的性能水平参数作为评判依据,找出造成损伤的因素及其达到的程度,同时获得损伤桥梁较为真实的性能水平,作为可靠性评估和加固设计的依据.通过对一座跨径160 m的PC连续刚构桥基于性能的损伤分析,结果表明:用该方法,结构挠度的校验系数可达0.9.     

火灾作用下钢筋混凝土框架结构的损伤机制

基于ABAQUS软件平台,开发了适用于钢筋混凝土框架结构火灾反应分析的梁单元用户材料模型及子程序,从而可以较为准确地模拟火灾下钢筋混凝土构件的截面温度场.基于塑性增量理论,对在恒定加载与标准升温耦合作用下的钢筋混凝土框架结构模型进行了非线性全过程分析.通过不同受火工况下的非线性全过程分析,揭示了钢筋混凝土框架结构在火灾高温和荷载共同作用下塑性铰的分布规律和结构损伤机制,分析了发生火灾的房间位置对框架结构破坏机制的影响,进而对发生火灾的最不利位置进行了探讨.分析结果表明,钢筋混凝土框架结构构件内力随受火温度升高不断重新分布,构件受热膨胀引起的约束内力与荷载内力叠加将导致其出现塑性铰;受火房间的位置对钢筋混凝土框架结构塑性铰的分布和破坏机制有明显影响,因此,结构抗火设计时应考虑受火房间的不利位置.     

西安地铁盾构施工对古城墙影响

针对西安地铁2号线盾构施工下穿古城墙问题,在考虑城墙损伤的前提下,采用三维弹塑性有限差分法FLAC 3D软件模拟了不同加固措施工况下盾构施工对西安城墙北门的影响,得出了盾构掘进过程中城墙的沉降规律,并对该规律进行了分析.研究结果表明,采取钻孔灌注桩及化学注浆复合加固措施可以有效地保证城墙安全,为西安地铁2号线的施工及保护古城墙提供了指导性的建议,并为日后有关黄土地区的盾构施工提供了参考.