HPLC法测定阳春滴丸中淫羊藿苷的含量

目的：通过试验摸索阳春滴丸的含量测定控制方法。方法：色谱柱：Hedra ODS-2（Size：250mm×4.6mm,Media：10nm5um）;乙腈：水（25：75）为流动相;流速0.8ml/min。检测波长270nm。结果：淫羊藿苷进样量在0.112~0.560μg之间与峰面积分值呈线性关系（r=0.9998）,平均加样回收率达98.85%。重复性试验RSD%值为0.55%。结论：所建方法可行,专属性、重复性好,可用于阳春滴丸的质量控制。     

等离子技术

所谓等离子技术,就是利用等离子体获得高温热源的一项技术。在化学工业中,利用等离子技术能实现一系列的反应过程。等离子体是指处于电离状态的气态物质,其中带负电荷的粒子(电子、负离子)数等于带正电荷的粒子(正离子)数。通常与物质固态、液态和气态并列,称为物质第四态。通过气体放电或加热的办法,从外界获得     

电子气体分析方法综述

本文概述了电子气体的种类、纯度以及其应用状况,综述了电子气体中几种常见杂质包括氧气、一氧化碳、二氧化碳、碳氢化合物、水分的不同分析方法以及几种特殊杂质氟化氢和金属粒子的不同处理方法,文中详细说明了各种分析方法如：比色法、黄磷发光法、电化学法、传感器法、电解法、露点法的测定原理以及具体的分析步骤,另外针对近年来发展的几种新型分析方法色谱-质谱、色谱-红外光谱、色谱-原子光谱做了简单介绍,指出了电子气体分析方法的重要意义及发展前景。     

Development of the fingerprints for the quality evaluation of Viscum coloratum by high performance liquid chromatograp

A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside     

Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry

We described the first results of a quantitative ultra performance liquid chromatographytandem mass spectrometry method for a novel antimicrobial peptide （phylloseptin, PSN-1）. Chromatographic separation was accomplished on a Waters bridged ethyl hybrid （BEH） C18 （50mm× 2.1 mm, 1.7 μm） column with acetonitrile-water （25：75, v/v） as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 （PSN-1） and m/z 340.7/165 （Thymopentin, IS）. Protein precipitation was investigated and the recovery was satisfactory （above 82%）. The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 lag/mL with r〉0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration.