沥青路面全深式冷再生施工工艺研究

全深式再生（Full Depth Reclamation）就是采用专用的就地冷再生设备．对沥青路面进行现场冷铣刨．破碎和筛分,（必要时）掺入一定数量的新集料、再生结合料、活性填料（水泥、石灰等）、水,经过常温拌和、摊铺、碾压等工序．一次性实现对沥青路面再生的技术,     

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template, double stranded cDNA of HNG with FLAG in its C-terminal was obtained, which was cloned into the plasmid pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time, the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG, 25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h, then cell morphology, MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h, cell viability decreased to (65.8±5.3)%, and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG, Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast, pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.     

鲨鱼鳃部共附生真菌中抗肿瘤活性化合物的研究

项目摘要：癌症是危害公众健康的宿敌．海洋生物共附生真菌参与宿主化学防御,产生的活性代谢产物新颖多样,是筛选具有抗肿瘤活性的新结构先导化合物的资源宝库．鲨鱼的鳃是一种“活的自航式微生物采样器”,栖居了包括共附生真菌在内的复杂微生物群落,是尚未被开发的海洋微生物新资源．而鲨鱼抗病力强、几乎不患癌症的现象一直引人注目,     

901-W无溶剂瓷釉涂料

901-W无溶剂瓷釉涂料是北京航材百慕新材料技术工程有限公司研发的产品。该产品是由环氧树脂、防锈颜料、改性固化剂、活性稀释剂、助剂等组成的环保、无毒无溶剂液体环氧涂料,适用于自来水厂、饮用水箱、船舶淡水舱、消防水箱、饮用水管线等钢结构防腐涂装。     

FRP和RPC在土木工程中的研究与应用

纤维增强复合材料FRP和活性粉末混凝土RPC在土木工程领域中的研究与应用得到了飞速发展。详细介绍了FRP和RPC的基本物理力学性能及其在国内外的发展概况。基于FRP和RPC的优异性能,将两者结合在一起有望形成一种新的具有优良物理力学性能的配筋混凝土结构形式而应用于实际工程。     

432 m活性粉末混凝土拱桥的设计

拟修建的巴卡尔桥为432m跨径的拱桥,位于里耶卡到塞尼的高速公路上,横跨巴卡尔海峡。该桥几乎完全由强度达到200MPa的活性粉末混凝土预制构件拼装组成。对该桥新颖的结构形式,施工工艺和富有美感的外形进行了描述与分析。     

汽车蓄电池老化原理及其在线学习算法

在汽车工业中铅酸蓄电池的老化现象非常普遍,如何能够提前识别出蓄电池老化以及制定相应的整车保护措施是当前研究的热点。本文介绍了蓄电池老化的背景和意义,几种常见的老化种类,阐述了蓄电池的相关老化信号定义以及开发算法。最后通过相应的标定来将相关的信号运用到整车的功能中并给实际用户带来收益。     

常温养护下正交异性钢板-RPC组合桥面力学研究

为了解决正交异性桥面板铺装破坏和钢桥面板开裂的问题,提出一种常温养护下正交异性钢板-活性粉末混凝土(RPC)组合桥面结构体系。基于某大桥建立局部有限元模型,并计算对比常温RPC组合箱梁、纯钢箱梁、高温RPC组合箱梁和普通混凝土组合箱梁的桥面系应力状态;同时开展局部模型静载试验。研究结果表明:常温养护下RPC抗压强度、抗折强度和弹性模量与普通混凝土相比有明显的提高;常温养护的RPC组合箱梁的RPC层拉应力达到了6.45 MPa,未出现裂缝,此应力远高于普通混凝土的抗拉强度,从而为解决桥面铺装破坏提供了思路;常温RPC组合箱梁和高温RPC组合箱梁桥面板应力降幅都超过了80%,明显大于普通混凝土组合箱梁,从而改善桥面板疲劳性能。常温养护的RPC在施工现场便于制作,应用前景较好。     

配筋活性粉末混凝土梁抗剪承载力分析

活性粉末混凝土(RPC)作为一种新型工程材料,其在工程中的应用前景越来越受到关注,但对其抗剪机理尚未完全认识.该文根据RPC的力学特性,基于Rankine破坏准则,推导了极限平衡法求解配筋RPC梁抗剪承载力的计算公式.该公式形式简洁,主要考虑了剪跨比、纵筋率和箍筋率等因素对抗剪承载力的影响.通过试验梁数据对比,验证了计...     

高压氧致大鼠氧惊厥时脑内氧自由基含量的变化

目的：对氧自由基（ＯＦＲ）在高压氧（ＨＢＯ）引致氧惊厥发生过程中的作用及其机理作初步探讨。方法：１０５只ＳＤ雄性大鼠被随机分为常压空气组、高压常氧氮组、ＨＢＯ组、ＶｉｔＣ－ＨＢＯ组、ＶｉｔＥ－ＨＢＯ组、ＳＯＤ－ＨＢＯ组、ＨＢＯ惊厥组、Ｖｉｔ－Ｃ－ＨＢＯ惊厥组、Ｖｉｔ－Ｅ－ＨＢＯ惊厥组和ＳＯＤ－ＨＢＯ惊厥组共２０个组。进舱前Ｖｉｔ－Ｃ－ＨＢＯｘｅｇ ，Ｖｉｔ－Ｅ－ＨＢＯ组、ＳＯＤ－ＨＢＯ组，Ｖｉｔ－