Unsupervised spatio-temporal segmentation for extracting moving objects in video sequences

Extraction of moving objects is an important and fundamental research topic for many video ap-plications. This paper addresses an unsupervised spatio-temporal segmentation scheme to extract moving objects from video sequences. The temporal segmentation localizes moving objects by comparing the motion vector of each block in each frame with the corresponding global motion vector estimated by an outlier rejection (OR) based method. Furthermore, the temporal compensation utilizing the temporal coherence of moving objects is considered in the temporal segmentation to solve the temporarily stopping problem. The detected moving regions usually have discontinuous boundaries and some holes. These regions are then compensated in the spatial domain. In the spatial segmentation, the watershed algorithm considering the global information improves the accuracy of segmentation in the spatial domain. The modified mean filter is presented to suppress some minima. By using a fusion module, moving objects are extracted. Experiments on various sequences have successfully demonstrated the validity of the proposed scheme.     

Facility Layout Based on Sequence Analysis： Design of Flowshops

A computer-aided method to design a hybrid layout—tree-shape planar flowlines is presented. In newtype flowshop layout, the common machines shared by several flowlines could be located together in functional sections. The approach combines traditional cell formation techniques with sequence alignment algorithms. Firstly, a sequence analysis based cell formation procedure is adopted; then the operation sequences for parts are aligned to maximize machines adjacency in hyperedge representations; finally a tree-shape planar flowline will be obtained for each part family. With the help of a sample of operation sequences obtained from industry, this algorithm is illustrated.     

Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50 : 1, 100 : 1) and concentrations (13. 0 pmol/25μL and 0.14 pmol/25μL, 19. 5 pmol/25μL and 0. 21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio of 50 : 1 provided better yield and concentration of 19. 5 pmol/25μL and 0. 21 pmol//25μL generated a clearer band. Conclusion A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.     

Cloning and sequencing of a DNA fragment encoding N37 apoptotic peptide derived from p53

Objective It was reported that p53 apoptotic peptide (N37) could inhibit p73 gene through being bound with iASPP, which could induce tumor cell apoptosis. To further explore the function of N37, we constructed the cloning plasmid of DNA fragment encoding p53 (N37) apoptotic peptide by using DNA synthesis and molecular biology methods. Methods According to human p53 sequence from the GenBank database, the primer of p53(N37) gene was designed using Primer V7.0 software. The DNA fragment encoding p53 (N37) apoptotic peptide was amplified by using self-complementation polymerase chain reaction (PCR) method and cloned into the pGEM-T Easy vector. The constructed plasmid was confirmed by endonuclease analysis and sequencing. Results The insertion of objective DNA fragment was confirmed by plasmid DNA enzyme spectrum analysis, p53 (N37) gene was successfully synthesized chemically in vitro. The sequencing result of positive clone was completely identical to the human p53(N37) sequence in GenBank using BLAST software (http://www. ncbi. him. nih. gov/cgi-bin /BLASTn). Conclusion The cloning of DNA fragment encoding p53(N37) apoptotic peptide was constructed by using DNA synthesis and pGEM-T Easy cloning methods. With the constructed plasmid, we could further investigate the function of N37 peptide.     

弹性需求和多用户随机平衡下的连续网络设计

连续平衡网络设计问题是在连续决策变量的条件下,寻找最优的用于道路网络中某些路段扩建的投资决策方案。文中从代表性消费者理论出发,建立了该问题的基于弹性需求和多用户类型随机用户平衡的双层规划模型,并考虑了路段能力约束。基于双层模型求解的复杂性,设计了基于混沌优化方法的启发式算法,实例计算结果表明该模型与算法是有效的。     

吉林省高速公路网规划建设项目实施序列优化

本文结合吉林省交通厅项目“吉林省高速公路网规划”,依据规划期公路发展的总目标和大布局,确定优化指标体系,使优化指标能全面反映公路网的全网经济性、交通功能性和社会功能性,同时,指标的口径对各公路网一致。运用本文提出的方法,对吉林省高速公路网规划建设项目进行为期5年的建设项目实施序列优化设计。     

基于自回归移动平均模型的梁桥损伤识别

桥梁结构的损伤识别是当前桥梁工程内十分活跃的研究领域,有着广泛的应用背景,与此相关的理论和技术也在不断的发展和成熟。基于自回归移动平均模型的研究方法以往都应用于结构的状态预测或系统识别,将其用于桥梁结构的损伤识别中还处于发展和完善阶段。拟结合桥梁结构的自身特点,对该方法在桥梁结构损伤识别中的应用做进一步的研究。     

基于VHDL的GOLD序列发生器的设计与实现

在分析m序列优选对的基础上,利用有限域法寻找一对优选对序列,采用VHDL语言实现一平衡GOLD序列,构造GOLG序列发生器.给出了流程图及仿真波形;通过EP1C3T144C-8芯片得到了较理想的测试波形,验证了该方法的可行性.     

一类超混沌系统的追踪控制与同步

针对一类新的超混沌系统,提出了一种追踪控制方案,利用Lyapunov方法证明了受控系统可使其状态输出指数收敛到任意给定的参考信号,实现同步．讨论了一个四阶超混沌系统的自同步及与Lorenz—Stenflo系统的异结构同步问题,数值仿真进一步验证该方法的有效性．     

峰峰值自适应延迟反馈混沌控制算法的改进

研究了混沌控制理论的时滞反馈控制算法及在该算法基础上改进的峰峰值自适应延迟反馈控制算法,得出以上算法在测量受到噪音干扰情况下,信号的峰值很难准确确定,而且控制稳定后,所得到的周期轨道不能很好重叠等不足,本文提出一种修正的峰峰值自适应延迟反馈算法,该算法通过事先给定延迟时间的变化阈值,来消除在自适应调节延迟时间过程中因测量误差带来的延迟时间的小幅波动,使轨道达到稳定的时间减小.最后使用该方法对自治Rossler系统进行控制,并用Matlab/Simulink工具箱对该系统进行了仿真验证.