Serum concentrations of laminin and fibronectin in patients with acute coronary syndromes

Objective To study the serum iaminin (LN) and fibronectin (FN) changes in acute coronary syndromes (ACS), and explore the role of them in assessing the severity of ACS. Methods This study included 46 ACS patients [25 with acute myocardial infarction (AMI) and 21 with unstable angina (UA)], 51 stable angina (SA) patients and 47 people without CHD as controls. Serum levels of LN, FN, fibrinogen and blood fat were assessed. Coronary angiography were performed on 49 of them. Results The serum concentration of LN was lower in ACS patients [(85.20±27. 57)ng/mL], higher in SA patients [(116. 80 ± 28. 80)ng/mL] as compared to that in the control group [(100.06±29.96)ng/mL], with significant difference among the groups (P<0.05). No difference was found in FN among the three groups. However, the subgroup analysis in the group with ACS showed that the serum concentration of FN was significantly higher in UA patients [(229.60±121.39)μg/mL ], and lower in AMI patients [(108.31±47.12) μg/mL ]. The serum LN and FN concentration could respectively enter the logistic regression equations of ACS patients and US patients. Neither LN nor FN concentration was correlated with narrowing of coronary artery of angiography. Conclusion Serum LN and FN level may be a useful indicator for stability of atherosclerosis plaque in coronary arterial disease patients, but could not predict the extent of narrowing in coronary angiography.     

The development of an OxyHb animal model in mice and the study on OxyHb-induced apoptosis of mouse brain cells in vivo

Objective On the basis of developing a new animal model for oxyhemoglobin (OxyHb) injection into subarachnoid space in mice, this research was to explore the temporal dependence and spatial distribution of OxyHb- induced apoptosis in the mouse brain cells in vivo and the mechanism of neurocyte injury induced by OxyHb. Methods The animal model for OxyHb injection into subarachnoid space in mice was developed. Mice were divided randomly into the experimental group (n=40) and the control group (n= 35). The control group received saline injection (50 μL ) and the experimental group received OxyHb injection (50 μL ), both into the subarachnoid space. The mice of the two groups were subdivided according to different postoperative time (3 h, 6 h, 12 h, 24 h and 48 h). The apoptosis or necrosis of cells was distinguished with microscopy (HE staining), transmission electron microscopy and TUNEL method. Results The distribution of apoptosis was mainly in the ipsilateral neocortex and bilateral hippocampal gyrus. The apoptotic mouse brain cells showed morphological changes in the experimental group by HE staining and transmission electron microscopy. The count of TUNEL-positive cells showed substantial increase in the experimental group, and there was a significant difference between the control and experimental groups, and the number of OxyHb- induced apoptotic cells decreased with time. Conclusion OxyHb in subarachnoid space in mice can induce apoptasis, but not necrosis of mouse brain cells in viro. The apoptotic brain cells show the pattern of temporal dependence and spatial distribution. It is suggested that the early treatment should be the method of first choice for treating the hemorrhagic brain injury.     

白藜芦醇在大鼠重症急性胰腺炎中的钙离子调节作用

细胞内钙超载在急性胰腺炎中发挥关键性作用,而白藜芦醇可以减轻胰腺炎的严重程度。有关白藜芦醇治疗胰腺炎的机制尚未明确。本研究旨在阐明细胞内钙离子调节失衡和白藜芦醇对大鼠急性胰腺炎的治疗效应间的关系。将雄性SD大鼠随机分成3组：假手术组（OS）、重症急性胰腺炎组（SAP）和白藜芦醇治疗组（RES）。通过胰胆管逆行注射牛磺胆酸钠建立重症急性胰腺炎模型,而白藜芦醇通过静脉给药。     

CD40基因多态性与自身免疫性甲状腺病的相关性

目的了解CD40基因多态性在西北地区汉族人群中的分布及其与自身免疫性甲状腺病(AITD)的相关性。方法随机选取本院内分泌科门诊的165例Graves病(GD)患者、113例桥本甲状腺炎(HT)患者为研究对象,以94例健康体查者为对照。①应用聚合酶链反应-限制性酶切片段长度多态性技术检测CD40基因5′非翻译区-1位点C/T多态性(5′UTR C(-1)T)、第三外显子58038位点-/T多态性;②应用扩增受阻突变系统-聚合酶链反应检测第九外显子64610位点C/G多态性。结果①5′UTR C(-1)T位点中CC基因型、C等位基因在GD组和对照组间有显著差异(P<0.05);②58038位点在372例样本中均为TT型;③64610位点未发现GG基因型。结论①西北地区汉族人CD40基因5′UTR C(-1)T位点上C等位基因型与GD发病有相关性,CC基因型者患GD的危险性较TT高;②58038位点-/T在西北地区汉族人中无多态性;③64610位点C/G在西北地区汉族人中未发现GG基因型。     

The clinical value of enzyme-multiplied immunoassay technique monitoring the plasma concentrations of cyclosporine A a

The feasibility and the clinical value of the enzyme-multiplied immunoassay technique （EMIT） monitoring of blood concentrations of cyclosporine A （CsA） in patients treated with CsA were investigated after kidney transplantation. The validation method was     

肝炎后肝硬化门脉高压症患者行脾切断流术后门静脉系统血栓形成的风险因素分析及处理

目的分析肝炎后肝硬化门脉高压症患者行脾切除加贲门周围血管离断术后门静脉系统血栓形成的相关因素及溶栓治疗效果。方法回顾分析西安交通大学医学院第一附属医院2005年1月至2011年12月收治的肝炎后肝硬化门脉高压症行脾切除加贲门周围血管离断术患者71例。根据抗凝剂使用情况将其分为3组:A组23人(术后早期应用抗凝药);B组29人(术后血小板>300×109/L时使用抗凝药);C组19人(术后未使用抗凝药)。比较各组门静脉系统血栓发生率和血栓分布特点以及发生血栓后经溶栓治疗后的效果,分析术前和手术相关因素与门静脉系统血栓形成的关系。结果脾静脉直径和术前低血小板计数是脾切断流术后门静脉系统血栓形成的独立风险因素。脾切断流术后门静脉系统血栓总的发生率为40.8%(29/71),A、B和C组分别为26.1%(6/23)、44.8%(13/29)和52.6%(10/19),各组之间差异均无统计学意义。脾切断流术后门静脉及主要分支均可发生血栓,其中脾静脉血栓占72.4%。溶栓治疗后门静脉和肠系膜上静脉血栓完全消融率为76.2%(16/21),而脾静脉血栓完全消融率仅23.8%(5/21),两者比较差异有统计学意义(χ2=11.524,P=0.001)。结论脾静脉直径和术前低血小板计数是肝炎后肝硬化门脉高压症患者行脾切断流术后门静脉系统血栓形成的独立风险因素。预防性使用抗凝药对降低门静脉系统血栓的发生率无显著作用。发生血栓后经全身溶栓治疗,门静脉和肠系膜上静脉血栓消融率高于脾静脉血栓消融率。     

Graves病动物模型干预模式的探讨

目的研究造模时间及免疫次数对促甲状腺激素受体(TSHR)A亚单位的重组腺病毒(Ad-TSHR289)诱导的毒性弥漫性甲状腺肿(Graves病)模型的影响。方法 45只BALB/c小鼠分别注射对照腺病毒(Ad-Lacz)或AdTSHR289,于第2次免疫后2周或第3次免疫后4周处死。所有小鼠均采取摘眼球取血,放免法检测血清促甲状腺激素受体抗体(TRAb)以及总甲状腺素(TT4)水平;剥离甲状腺,进行组织学检查。结果所有注射Ad-TSHR289的小鼠相对于对照组TRAb均明显升高;5周-造模组、10周-造模组的Graves病发病率分别为56%(9/16)、75%(9/12);10周-造模组甲状腺组织学改变与TT4水平变化吻合度为100%,相对于5周-造模组的50%(8/16)明显升高。结论 10周-造模组相对于5周-造模组具有发病率高、组织学改变与血清TT4水平吻合度高等优势;造模时间以及免疫次数均对该Graves病模型有着较大的影响。     

综合汽车维修企业疑难病症解析（二十）——汽车维修企业“脑梗”病症的治疗（2）

编制企业未来5年经营规划上期指出＂脑梗＂病症是大脑的思维病症,大脑缺乏对企业的战略思维。在上期完成了七个项目分析的基础上,本期进入企业战略计划的编制工作,包括：①企业的产品的定位和品牌发展方向;②企业的财务发展规划（维修台次、收入、成本、毛利规划）;③企业的市场占有及客户保有量增长规划;④员工的生产效率与质量规划;     

克山病患者红细胞免疫功能与血硒水平的关系

对潜在型、慢型克山病患者和病区健康居民的红细胞免疫功能、血清红细胞免疫粘附调节因子、循环免疫复合物（CIC）、全血硒含量和全血谷胱甘肽过氧化物酶活性（GPX）进行了检测，结果是：潜在型、慢型克山病患者和病区健康居民红细胞C3b受体花环率、全血硒含量和全血GPX活性均明显低于非病区居民；血清红细胞免疫粘附抑制率均明显高于非病区居民；全血硒含量与红细胞C3b受体花环率呈显著正相关（r=0．887，n=61，P＜0．01）；与血清红细胞免疫粘附抑制率呈显著负相关（r=0．781，n=60，P＜0．01）；而免疫复合物（IC）花环率、血清红细胞免疫粘附促进率和CIC在各型克山病患者、病区健康居民及非病区居民之间无明显差异。提示：潜在型、慢型克山病患者和病区健康居民红细胞免疫功能低下，可能与低硒状态和血清红细胞免疫粘附抑制因子增加有关。