尿毒症apoE基因敲除小鼠主动脉动脉粥样硬化的病变特征

目的 观察尿毒症apoE-/-小鼠主动脉粥样硬化(As)病变特征及MMP-9的表达.方法 以apoE-/-小鼠为研究对象,通过2步外科手术法建立尿毒症模型,对照组行假手术.造模后检测肾功能,判断造模是否成功.造模后10周,检测血清MMP-9浓度;主动脉根部切片油红O染色计算斑块相对面积,天狼星红染色计算斑块内胶原含量,α-肌动蛋白、MAC3免疫组化染色检测斑块内SMC及巨噬细胞的含量;RT-PCR法检测主动脉MMP-9 mRNA的表达.结果 造模后肾功能检测显示造模成功.尿毒症组与对照组相比:主动脉根部As斑块相对面积提高、斑块内胶原含量减少、SMC成分减少、巨噬细胞含量增加,血清MMP-9浓度及主动脉MMP-9 mRNA的表达提高.结论 尿毒症apoE-/-小鼠主动脉加速发展的As伴随着斑块的稳定性降低.     

宁夏回族高脂血症患者CYP2C9*3、MSA2756G基因多态性的研究

目的 研究高脂血症治疗的药物相关基因CYP2C9*3、甲硫氨酸合成酶(MSA2756G)在宁夏回族高脂血症患者中的分布及其与高脂血症的关系.方法 通过扩增引进限制性酶切位点(ACRS)和应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对高脂血症患者进行基因型分析.结果 180名宁夏回族高脂血症患者中,CYP2C9*3等位基因频率为3.33%,突变频率男性(3.05%)高于女性(0.28%),差异有显著性(P<0.01),而与健康对照组(3.00%)比较,差异无显著性(P>0.05);MSA2756G等位基因频率为15.83%,显著高于健康对照组(10.25%)(P<0.05),未发现性别差异.结论 宁夏回族人群携带CYP2C9*3基因型的男性患高脂血症风险更大;MSA2756G可能是高脂血症的危险因子.     

Association between the -1562 C/T polymorphism in the MMP-9 promoter and phenotype of esophageal squamous cell carcinoma in northern Chinese population

Objective To conduct a case-control study on the association of the nucleotide polymorphisms in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene with phenotype of esophageal cancer. Methods All subjects were unrelated residents in northern regions of China. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the MMP-9 genotypes. Results The overall distribution of genotypes in the patients was not different from that in the controls (OR=0.77, 95% CI=0.45-1.34; P=0.36). There were no significant differences between the patients and the control subjects in terms of the distributions of sex and age, smoking status, alcohol dependence, pickled diet status, or history of environmental exposure. The patients were further examined with stratifications by age, sex, grade, depth of tumor invasion, lymphatic invasion, venous invasion and TNM staging. The results showed no pronounced association among the stratifications. Conclusion There is no significant association between the MMP-9 single nucleotide polymorphism genotypes and phenotype of esophageal cancer.     

单片机在铁路桥梁挠度监测中的应用

桥梁挠度监测是山区铁路安全领域的重要组成部分,是保障行车安全的一项重要指标.针对桥梁的特殊结构,开发了一种基于高性能ARM处理器、电荷耦合器件,以及RS-485串行通信总线的挠度测量装置.能根据远程控制计算机的选项输入指令,自动完成数据的采样,运算和传输,实现挠度的实时查询,可图表显示,测量精度高,是挠度监测的新方法.     

基于MS9S12DG128单片机的电动汽车电池管理系统设计

根据动力电池组在电动汽车上的使用特点和要求,采用了带MSCAN、且具有强大功能模块的嵌入式微处理器MS9S12DG128,完成了电动汽车用电池管理系统的硬件设计,实现了对电压、电流、温度的实时精确采集、SOC的精确估计和CAN通讯。实验表明,本系统各方面性能优越。     

路侧交通流量雷达系统分析与设计

根据实际提出了车流量检测雷达系统的性能技术参数要求,确定了系统实际的工作频段和工作体制,并进行了方案论证。提出了一套基于ARM和DSP双处理器的路侧雷达交通流量监控系统,给出了系统软、硬件结构设计方案,为交通流量雷达系统设计提供完整的理论指导和参考。     

基于ARM的数据采集系统设计

介绍基于嵌入式计算机系统的数据采集系统的设计和实现,该系统由硬件和软件两部分组成.硬件采用基于ARMv5te、XScale构架的嵌入式微处理器PXA270芯片,软件采用嵌入式操作系统Linux.与传统的数据采集系统相比,此系统具有集成度高、组合灵活、更好的可靠性和实时性,目前已经用于400 km/h高速综合检测列车上应答器的数据采集,同时本系统也可以应用于实时图像监控等复杂系统中.     

电动汽车无刷电机控制系统的设计

为对无刷直流电机实现开/闭环两部分控制,文章介绍了基于Freescale 9S12XS128芯片实现对电动汽车无刷直流电机控制器的设计。程序的编译采用了Codewarrior软件,闭环控制即在车上安装了振荡传感器,可根据不同的振荡程度自行调整无刷电机的转速。开环控制是外设有滑动变阻器,用户可以通过调整滑动变阻器来控制输入电压信号的大小,进而调整PWM的输出来控制电机的转速。此款控制器实现了电机的开/闭环控制,还设计有显屏,可显示速度、电流、电压及消耗的电量,且预留有无线输出口并配有LabVIEW上位机软件可用于调试电机和采集数据。     

Environmental innovation and the role of stakeholder collaboration in West Coast port gateways

The paper explores the role of stakeholder collaboration in the adoption of innovations as part of the environmental and sustainability agenda of port gateways. We do this through a comparative assessment of the port of Vancouver, British Columbia, and the twin ports of Los Angeles and Long Beach, California. An inductive research approach is used to identify and assess the initiation and implementation process behind exemplary innovations. Innovation includes new technologies and processes for handling and moving cargo, mechanisms for planning and policy making, as well as financing, implementing, upgrading, managing and operating infrastructure systems. A relatively new arena for competition on the basis of innovation concerns environmental performance. The conceptual framework and empirical evidence suggest that while there may be intense demand for and supply of innovation in port gateways, the complex dynamics of the logistics chain are such that successful innovation requires conscious involvement and collaboration of stakeholders.     

GPS坐标成果的游动九参数转换法

介绍Delaunay三角形的基本概念,提出大区域GPS坐标成果转换采用基于Delaunay三角形的游动九参数转换法,利用该法对实际GPS控制网坐标成果进行转换,取得了较理想的结果,为大区域的GPS坐标成果转换提供了一种可行的方法。