FOB卖方作为托运人的法律地位及其权利

从我国《海商法》对托运人的规定出发,结合FOB国际货物买卖的实际情况讨论其卖方作为托运人的法律地位及其权利与义务。     

第三方物流企业信息平台的研究与开发

分析了港口发展第三方物流及构建其物流信息平台(LIP)的必要性，对策三方物流企业LIP的开发方法和应用体系结构进行了探讨，从功能需求、系统平台及开发工具选择、数据库平台设计和程序实现等方面对LIP的开发过程进行了阐述。     

浙江省B2C电子商务企业物流模式探讨

B2C电子商务企业能取得多大的成功很大程度上依赖于实际物流的操作,B2C电子商务企业必须根据自身情况,正确选择和采用企业自营物流模式或是第三方物流模式。本文根据实地调研的数据,就浙江省B2C电子商务企业的物流模式存在的问题进行分析探讨,寻求成本低廉、需求响应快速、安全可靠且适宜的物流模式。     

基于ASP的在线考试系统的设计及应用

一个完整的在线考试系统应该包括考试用户注册系统、在线考试系统、分级账户及试卷管理系统、用户成绩查询系统、帮助系统等。本文提出了一种基于Asp的在线考试系统解决方案,具有移植性好、易于实现等优点。     

浅析呼和浩特西站计算机联锁改造

本文介绍了呼和浩特西站(DS6-K5B 计算机联锁系统)与呼和浩特站增加三线工程改造施工的相关过程和经验,重点阐述了大型站场含控制台改造需要注意的问题。     

Fleet F-77在货物链海运环节应用的探讨

在全球化经济日趋一体化的今天，一旦货物链中的某个环节发生故障将产生连锁反应，导致多个国家地区以至全球经济的重创。海运由于其特殊性在当今货物链中扮演着极其重要的角色，而海运系统又是货物链中的脆弱环节，如何保证海运环节的通畅是保证整个货物链不断的关键。本着上述观点，探讨了Inmarsat新近推出的FteetF-77在保障海运通畅、保证全球货物链不断方面的重要应用，希望新技术业务的推出能对全球经济发展起一定的作用。     

Methylation status of the interferon-gamma gene promoter in chronic hepatitis B

Objective To evaluate the methylation status at CpG site -55 in the interferon-gamma (IFN-7) gene promoter and its effect on IFN-7 expression in chronic hepatitis B. Method The authors recruited 30 patients with UBeAg-positive chronic hepatitis B (CHB), 30 HBeAg-negative CHB patients, and 30 healthy blood donors. Pyrosequeneing was used to determine the methylation status at CpG site -55 in the IFN-γ gene promoter following bisulfite treatment of DNA in peripheral blood mononuclear cells (PBMCs). The expression of IFN-γ was analyzed by real-time RT-PCR and ELISA. HBV DNA in PBMCs was detected by nested PCR. Results The methylation level at CpG site -55 in the IFN-γ gene promoter was significantly increased, resulting in subsequent down-regulation of the expression of this cytoldne in CHB. The methylation level at CpG site -55 was significantly higher in HBeAg-positive patients than in HBeAg-negative ones (P<0.01) and was also significantly higher in PBMCs from HBV DNA-positive patients than from HBV DNA-negative ones (P<0.01) ; the methylation level at CpG site -55 was positively correlated with the amount of HBV DNA in serum (P<0.01). Oonclusion IFN-γ gene expression appears to be regulated by methylation of the IFN-γ gene promoter in CHB; the methylation level at CpG site -55 is associated with HBV infection.     

Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.     

间接法和捕捉法检测特异性IgM的敏感性与特异性的比较

目的比较捕捉法和间接法检测IgM时的特异性和敏感度差异,并探讨其产生差异的原因。方法分别用间接ELISA法和捕捉ELISA法检测活动性乙型肝炎和肾综合征出血热(HFRS)患者标本的IgM水平,并比较两者之间检出率、检测阈值、特异性的差异。结果检测稀释在PBS中的鼠抗IgM特异性抗体,两种方法的特异性和敏感度类似;检测活动性乙型肝炎和HFRS患者标本,间接法检测IgM的敏感度明显低于捕捉法;检测经二巯基乙醇处理后乙肝组和HFRS组标本,两种检测均为阴性,表明两种方法都具有针对IgM抗体的型特异性;交叉实验结果证明两种检测IgM的试剂均具有针对抗原的特异性。结论急性感染疾病的早期诊断、病毒的潜伏期感染和慢性感染的再次激活应使用捕捉法检测IgM,间接法检测IgM用于急性感染性疾病的诊断有待进一步探讨。     

桥梁养护管理系统的设计与开发

通过对桥梁养护数据管理、安全状态评估、养护辅助决策分析、信息共享等需求进行分析的基础上,应用Web-GIS技术和基于B/S网络结构技术对桥梁养护管理系统(BMMS)进行了设计,给出了系统结构、功能模块和养护评估模型;应用面向对象的软件开发技术研制了基于区域性管理的桥梁养护管理系统。将所设计的方案和研制的软件应用于江苏省汾灌高速公路桥梁养护的实际工程中,结果表明,桥梁缺损状况的评估结果与江苏高速公路工程养护有限公司技术工程师的评估结果一致,证实了所开发系统(BMMS)的可操作性,并实现了各个职能部门的信息共享和桥梁养护的区域性管理。