广州地铁4号线列车EP2002制动系统介绍及故障分析

对广州地铁4号线列车EP2002制动控制系统特点进行了简要描述,详细介绍4号线列车EP2002制动系统各项功能,对EP2002阀之间的通讯,以及EP2002阀同列车线、TMS和VVVF之间的通讯进行了介绍,结合列车在运营中出现的故障进行了分析。     

车辆隔热壁K值计算中空气层的处理方法

对轨道交通车辆多层复合结构的隔热壁进行隔热性能理论计算与仿真计算的对比,对隔热壁内部空气层的隔热性能进行分析。结论为:在车辆多层复合结构的隔热壁K值计算中,当空气层厚度大于一定厚度时,流体自然对流对隔热壁的隔热性能影响较大。利用多项式密度(Polynomial Denisity)和Boussinesq模型两种算法,对所得结论进行了验证。     

Investigation of tailpipe and evaporative emissions from China IV and Tier 2 passenger vehicles with different gasolines

The limited understanding of vehicular emissions in China, especially evaporative emissions, is one obstacle to establishing tighter standards. To evaluate tailpipe and evaporative emissions, two typical China IV vehicles and one Tier 2 vehicle with an onboard refuelling vapour recovery (ORVR) system were selected and tested. One of the China IV vehicles was fuelled with gasoline, E10 and M15, respectively, to investigate the effect of fuel properties on vehicular emissions. For each vehicle, cold-start tailpipe emission tests were conducted first, followed by an evaporation test. Based on the emission factors and real-world vehicle activity data, the annual tailpipe and evaporative hydrocarbon (HC) emissions of each vehicle were calculated and compared. The results show that E10 and M15 significantly reduced the tailpipe CO and particle number (PN) emissions but seriously aggravated the NOx emissions, especially for M15. The hot soak losses (HSLs) and diurnal breathing losses (DBLs) were slightly impacted by the fuel properties. The annual evaporative emissions with E10 and M15 were higher than that with gasoline. The ORVR system effectively controlled the evaporative emissions, especially for DBLs. Evaporative emissions from the China IV vehicles were 1.1–1.4 times the tailpipe HC emissions. Additionally, the evaporative emission factors of the China IV vehicles were almost 50% lower than the standard (2.0 g/test), whereas their annual evaporative emissions were almost 1.8–2.8 times higher than those from the Tier 2 vehicle. Therefore, controlling evaporative emissions currently remains a great need in China, and the ORVR might be a recommended evaporative control technology.     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

mTOR/P70S6K信号通路在宫颈癌组织中的表达及其临床意义

目的 研究mTOR/P70S6K信号通路中哺乳动物雷帕霉素靶蛋白(mTOR)及其底物激酶(P70S6K)在宫颈癌中的表达及其在宫颈癌发生发展中的作用.方法 用RT-PCR和免疫组织化学法检测正常宫颈、宫颈癌中mTOR和P70S6K激酶的表达.结果 与正常组织相比,宫颈癌中的mTOR和P70S6K激酶在mRNA及蛋白水平表达均显著增加(P<0.05),且两者的表达具有显著的正相关性(r=0.746,P<0.001).结论 mTOR/P70S6K信号转导通路在介导宫颈癌的发生、发展的过程中起到重要作用.     

Observations of the Fawn Trough Current over the Kerguelen Plateau from instrumented elephant seals

Due to its great meridional extent and relatively shallow depths, the Kerguelen Plateau constitutes a major barrier to the eastward flowing Antarctic Circumpolar Current in the Indian sector of the Southern Ocean. While most of the Antarctic Circumpolar Current transport is deflected north of the Kerguelen Islands, the remainder ( 50 Sv, 1 Sv = 10⁶ m³ s^− 1) must pass south of the islands, most probably through the Fawn and Princess Elizabeth Troughs. However, the paucity of finely resolved quasi-synoptic hydrographic data in this remote and infrequently sampled area has limited the progress in our knowledge of the regional circulation. Since 2004, a new approach using elephant seals from the Kerguelen Islands as autonomous oceanographic profilers has provided new information on the hydrography over the Kerguelen Plateau, covering the entire Antarctic Zone between the Polar Front and Antarctica, with a mean along-track resolution of about 25 km. These finely resolved bio-logged data revealed details of a strong northeastward current found across the Fawn Trough (sill depth: 2600 m; 56°S, 78°E). This so-called Fawn Trough Current transports cold Antarctic waters found mostly south of the Elan Bank, between the Ice Limit (58°S) and the Antarctic Divergence (64°S) in the eastern Enderby Basin, toward the Australian–Antarctic Basin. Our analysis also demonstrates that the Deep Western Boundary Current, which carries cold Antarctic water along the eastern flank of the southern Kerguelen Plateau collides with Fawn Trough Current at the outlet of the Fawn Trough sill. In other words, the Fawn Trough constitutes a veritable bottleneck, channelling the quasi-totality of the Antarctic Circumpolar flow found south of the Polar Front. Thanks to the unprecedented fine resolution of seal-borne data, a branch of flow centered at the Winter Water isotherm of 1 °C is also revealed along the northern escarpment of the Elan Bank, and then along the southern edge of Heard Island. Further analysis of different supplementary data reveals a complex circulation pattern in the entire Enderby Basin, with several distinctive branches of flow being strongly controlled by prominent topographic features such as the Southwest Indian Ridge, Conrad Rise, Elan Bank, and Kerguelen Plateau. This newly emerged frontal structure refines considerably previous large-scale circulation schematics of the area.     

一种单片机控制的水温测试系统

由于现代多样的电子控制技术和电子控制理论的迅猛发展,高精度的机械在各类工业控制系统、生产线等方面的需求越来越大.其中以单片机为核心实现的数字控制器,因其体积小、简便易行等优点,而被广泛应用于各行各业.本文将单片机的温度控制系统作为设计核心,通过温度传感器、单片机等软件系统图,并利用软件编程,从而达到实现水温控制的目的....     

智能马达控制中心在海洋平台设计中的应用

介绍一种利用新型智能电机过载继电器E3 PLUS、现场通信总线DeviceNet及PLC等技术相结合的船舶智能马达控制系统,阐述智能马达控制中心(Smart MCC)的工作原理、功能及应用实例,该系统可以提供电机多种故障检测和诊断功能以及计算机集中控制,使马达控制系统逐步向自动化集成型发展,系统的性能和可靠性高,能最大限度地降低船舶整体运行成本,提高运行效率.     

RS20型交换机的配置方法

对RS20交换机的配置进行探索研究具有很强的实用性,重点介绍了RS20交换机在轨道交通无线通信系统中的应用,通过不断的实践摸索,初步掌握了一套快速配置RS20交换机的方法,并对这种配置方法进行了探讨.     

自卸式远洋散装水泥运输船特殊结构强度分析研究

以远洋散装水泥运输船为研究对象,采用三维有限元分析方法及ＤＮＶ船级社的ＳＥＳＡＭ结构计算软件,从船体结构强度、船舶振动预报和船体结构温度应力等方面对该型船结构设计的多项技术难点进行了详细的计算和评估。并且着重对大型散装水泥运输船间断内底板、中纵舱壁以及高腹板旁桁材等特殊结构的受力状态及应力分布进行分析和探讨。为自卸式散装水泥运输船的结构优化设计提供技术支撑。