PtenL/LMEFs与Pten△/△MEFs细胞系的差异表达蛋白质组分析

目的研究PtenL/LMEFs与Pten△/△MEFs细胞的蛋白表达差异。方法运用双向电泳技术比较PtenL/LMEFs与Pten△/△MEFs细胞的蛋白表达差异,6个差异蛋白进行胶内酶切后运用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)进行质谱分析,肽质量指纹图谱检索数据库后5个蛋白得到鉴定。结果同对照PTEN野生型细胞PtenL/LMEFs相比,磷酸甘油酸变位酶1(phosphoglycerate mutase 1,PGAM1)和肽脯氨酰顺反异构酶C(pepti-dyl-prolyl cis-trans isomerase C,cyclophilin C)在Pten△/△MEFs细胞中表达上调而Transgelin 2蛋白在Pten△/△MEFs细胞中表达下调。经鉴定已知蛋白点B和F都是过氧化物氧化还原酶6(Peroxiredoxin-6),蛋白点B在Pten△/△MEFs细胞中表达而在Pten△/△MEFs细胞中检测不到,蛋白点B和F的分子质量相似而等电点不同,可能是由于翻译后修饰。结论同对照PTEN野生型细胞PtenL/LMEFs相比,PTEN缺失的Pten△/△MEFs细胞有明显的差异性蛋白表达谱。PTEN的缺失引起细胞内多种蛋白表达改变,这些表达改变的蛋白可能与PTEN缺失后细胞癌变相关。     

Study on DSM-based task planning of product cooperative development

The results of analyzing the managerial characteristics and complexity of product cooperative development suggest that task planning is an important aspect for process management of product cooperative development and the method for planning tasks should be able to model the dependency between tasks and iterations during the development process. In this paper, a DSM-based method and its corresponding optimization algorithms are developed. At first the coupled task sets and uncoupled task sets are identified, and the tasks are then optimized by the corresponding algorithms. The optimal tasks plan will reduce the development time and cost. Considering the practical requirements in real world, a Multilayer DSM is proposed, and its information communication techniques between DSM and traversing principle are described in details.     

剪切效应梁单元刚度和质量矩阵的推导及应用

从工程梁理论中的梁位移微分方程出发直接推导出考虑剪切效应的空间梁单元的刚度矩阵,并从动力荷载的虚功原理出发推导出考虑剪切效应的其质量矩阵。编制了简单的有限元程序去验证,通过计算实例分别比较考虑剪切效应和不考虑剪切效应对结构固有频率的影响,结果表明在剪切系数较大时,不考虑剪切效应会引起较大的误差。     

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对于快递企业而言,快递过程总是与快捷运输工具的使用紧密联系的。因此,获取相适应的运力资源是其生存发展的基础。现实中,没有一家快递企业可以做到运力资源的完全内部化。这也就表明,快递企业与掌握各种运力资源的专业运输企业之间的合作似乎是必须的。社会经济系统中,存在大量的不确定性,且决策主体是有限理性的。快递企业与专业运输企业之间相互交往的规则或制度安排不是唯一的。本文试图用演化博弈模型来分析快递企业和专业运输企业的交互演进的过程中,通过对众多的参与者（多家快递企业和多家专业运输企业）的战略选择和调整的过程的动态分析,指出快递企业——专业运输企业动态合作过程的存在两个局部均衡点,也就是说,快递企业和专业运输企业合作模式的博弈有两个演进方向,既可以采取股权合作策略,也可以采取契约合作策略,这两种策略都是演化稳定策略。     

H型钢支模体系在青山湖隧道结构中的应用

对隧道钢筋混凝土箱涵结构、外侧墙体采用H型钢桁架支模体系结构的可靠性进行了充分的技术论证,应用结构有限元法对支模体系进行了静力分析计算．计算结果表明,该支模体系的结构承载力和刚度均满足现行国家规范要求．在将该模板体系应用于青山湖下穿隧道箱涵结构的施工过程中,通过对模板体系的侧向变形的实际监测,实测值均小于理论计算值。说明该支模体系应用在该隧道工程的施工中是完全可行的．     

基于灰关联分析的动态混合型多指标决策

研究了一类只有部分指标权重信息且指标值为精确数、区间数和语言类模糊数相结合的动态混合型多指标决策问题,提出一种基于灰矩阵关联度的动态混合型多指标决策方法,并给出一个具体的投资决策实例,结果表明:该方法是有效和可行的,从而为解决动态混合型多指标决策问题提供了一种新的途径.     

Underwater digital terrain model with GPS-aided high-resolution profile-scan sonar images

The whole procedures of underwater digital terrain model (DTM) were presented by building with the global positioning system (GPS) aided high-resolution profile-scan sonar images. The algorithm regards the digital image scanned in a cycle as the raw data. First the label rings are detected with the improved Hough transform (HT) method and followed by curve-fitting for accurate location; then the most probable window for each ping is detected with weighted neighborhood gray-level co-occurrence matrix; and finally the DTM is built by integrating the GPS data with sonar data for 3D visualization. The case of an underwater trench for immersed tube road tunnel is illustrated.     

Theoretical studies of active power/angle sub-matrix in power flow Jacobian for power system analysis

Properties of the active power/angle sub-matrix in the power flow Jacobian for power system analysis are studied. The sub-matrix is a dominant and irreducible matrix under very general conditions of power systems, so that it is invertible. Also the necessary conditions for its singularity are given. These theoretical results can be used to clarify the ambiguous understanding of the sub-matrix in current literature, and also provide the theoretical foundations for the applications based on reduced power flow Jacobian. Numerical simulation on the IEEE 118-bus power system is used to illustrate our results.     

服务台可修的离散时间GI/G/1重试排队系统

讨论了服务器可修的离散时间GI/G/1重试排队系统,其中重试时间服从Bernoulli分布,服务器的寿命为几何分布而修理时间为一般分布.将该系统转化为一个水平相依的拟生灭过程(QBD)并通过矩阵分析方法(MAM)进行分析.通过算法进行逼近,将一个水平相依的Markov链转化为一个有较大边界的与水平不相依的Markov链.最终得到了重试空间中顾客人数的分布,并且通过一些数值算例进一步说明了不同参数对系统的影响.     

基于马尔可夫链的燃气管道外防腐层预测

介绍了埋地燃气管道外防腐层老化状况评价标准,以沥青外防腐层为例,通过应用马尔可夫链理论,阐述了基于逆阵的外防腐层老化状况预测的方法,从而为确定整条管线外防腐层老化状况分布提供了理论依据。