STAAD.Pro在海洋工程模块吊装设计中的应用

对STAAD.Pro软件在海洋工程模块建造的加工设计阶段的应用进行了探讨。通过STAAD.Pro软件的可视化操作平台,对实际海洋工程模块进行建模、加载、计算和分析,通过计算得出模块的质量和质心位置,并根据规范对模块进行整体强度校核,从而对海洋工程模块的吊点布置及其吊装过程起到实际的指导作用。     

船舶低硫柴油系统设计的分析与优化

就新辟航线首制船“汉亚直达”集装箱船的低硫柴油系统,叙述了船舶低硫柴油系统的设计经验,从当前国内外对船用燃油硫含量的要求、应对方案到船舶低硫柴油冷却方式选择、低硫柴油冷却系统设计、高/低硫柴油转换、使用低硫柴油风险分析及处理等方面进行详细叙述,为业内同行提供参考。     

Tyre/road noise damping characteristics using nomographs and fundamental vibroacoustical relationships

Several studies have successfully developed laboratory and field measurement techniques to estimate tyre/road noise damping characteristics. However, laboratory–field noise correlations of pavement types in the form of nomographs is essential to cognize the pavements’ acoustical properties from a practical perspective. A toolkit that readily provides field tyre/road noise intensity of different pavement materials is needed. Thus, the main objective of this study was to develop nomographs with relationships for tyre/road noise versus pavement materials’ viscoelastic–vibroacoustical properties. Isothermal and isochronal nomographs of phase angle–tyre/road noise as toolkits were developed based upon well-established phase angle and noise intensities typical of mixtures, making the nomograph correlations very authentic, realistic, and novel along with benefit to predict tyre/road noise at any desired temperature and traffic speed (or frequency) combination. This study is envisaged to benefit in the discernment of road materials’ damping capabilities from vibroacoustical and field noise–viscoelastic relation aspects.     

船舶推进同步电机PTI/PTO模式研究

PTI/PTO推进是最近发展起来的一种新型船舶推进方式,兼具传统柴油机推进和电力推进优点,可实现多种船舶工作模式,具有操控性好、安全、经济等特点,将是未来船舶推进方式发展方向之一.本文介绍了PTI/PTO推进模式理论和工作模式,便于理解该推进方式,希望有助于船舶推进技术的发展.     

STAAD/CHINA在搭架设计及强度校核中的应用

搭架作业是船舶修造日常工作中比较普通、常见的工程,合理、快速地设计和校验脚手架的强度,直接影响生产施工的安全性、经济性和效率。文章介绍了如何使用有限元软件STAAD/CHINA对搭架方案进行建模计算及校验,计算结果接近实际,提高了强度校验的效率。     

Dynamic demand estimation and prediction for traffic urban networks adopting new data sources

Nowadays, new mobility information can be derived from advanced traffic surveillance systems that collect updated traffic measurements, both in fixed locations and over specific corridors or paths. Such recent technological developments point to challenging and promising opportunities that academics and practitioners have only partially explored so far.The paper looks at some of these opportunities within the Dynamic Demand Estimation problem (DDEP). At first, data heterogeneity, accounting for different sets of data providing a wide spatial coverage, has been investigated for the benefit of off-line demand estimation. In an attempt to mimic the current urban networks monitoring, examples of complex real case applications are being reported where route travel times and route choice probabilities from probe vehicles are exploited together with common link traffic measurements.Subsequently, on-line detection of non-recurrent conditions is being recorded, adopting a sequential approach based on an extension of the Kalman Filter theory called Local Ensemble Transformed Kalman Filter (LETKF).Both the off-line and the on-line investigations adopt a simulation approach capable of capturing the highly nonlinear dependence between the travel demand and the traffic measurements through the use of dynamic traffic assignment models. Consequently, the possibility of using collected traffic information is enhanced, thus overcoming most of the limitations of current DDEP approaches found in the literature.     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

快速老化小鼠的学习记忆缺陷及其生化机制

目的用快速老化小鼠(SAMP8)模拟老年痴呆,观察其学习记忆能力,并探讨其学习记忆能力下降的可能机制。方法采用10月龄SAMP8,并用同龄抗快速老化小鼠(SAMR1)作为正常对照。用水迷路检测近记忆以及空间学习记忆能力。用生物化学方法检测大脑皮层线粒体膜电位和ATP酶活力,观察线粒体功能;检测皮层和海马胆碱乙酰基转移酶(ChAT)和乙酰胆碱酯酶(AChE)的活力,观察中枢胆碱能神经功能;检测血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,观察其全身性氧化应激状况。结果与SAMR1相比,SAMP8出现明显的衰老体征,其近记忆和空间学习记忆能力明显减弱,具有衰老和痴呆特征;皮层线粒体膜电位和ATP酶活力显著降低,海马ChAT活力显著下降,AChE活力显著升高,血清SOD活力稍有增高。结论SAMP8可以很好地模拟老年痴呆,其机制包括大脑皮层线粒体功能降低、海马胆碱能神经功能下降以及氧化应激等。     

基于ASP.NET的B/S架构的软件在Windows7系统下的部署

本文重点研究了对于B/S架构的软件在Windows7系统下部署时应该采用的一系列方法及在部署时遇到的一系列问题的解决方案。文中分析了利用ASP.NET开发的B/S架构的软件的优点并在此基础上介绍了软件部署的流程。本文在分析过程中结合具体实例提出了部署中一些常见问题的解决方案。     

姜黄素促进RAW264.7源性M1巨噬细胞向替代激活M2表型极化

目的观察姜黄素对LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)极化的影响及机制。方法不同浓度姜黄素(6.25、12.5、25μmol/L)干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,同时再加入20μmol/L GW9662与25μmol/L姜黄素共同干预LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)12h,采用Real-time PCR、ELISA及Western blot方法检测IL-1β、IL-6和M2表型标志分子KLF4、FIZZ1、MGL1、PPARγ的表达,及阻断PPARγ后KLF4和FIZZ1的表达。结果不同浓度姜黄素均能上调LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)的M2标志分子的表达,并且抑制炎症因子IL-1β和IL-6的分泌;阻断PPARγ后,RAW264.7巨噬细胞源性M1表型巨噬细胞表达M2标志分子下调。结论姜黄素通过活化PPARγ促进LPS和IFNγ诱导的RAW264.7巨噬细胞(M1)向M2表型极化。