车板耦合作用对水泥砼路面疲劳开裂的影响研究

水泥砼路面开裂和破坏的影响因素较多 ,情况也比较复杂 .笔者首先分析路面板在运动荷载作用下的动力响应 ,建立粘弹性地基上板的动力模型 ,然后从理论上探讨路面不平整引起的车板耦合作用对路面板的挠度、应变及其路面疲劳开裂的影响 ,从而为水泥砼路面的抗裂研究提供依据     

酵母双杂交系统筛选HBV PreS1相互作用蛋白

目的利用Sos招募系统(SRS),构建含HBV PreS1基因的酵母双杂交诱饵载体,筛选人肝细胞与HBV PreS1蛋白相互作用的蛋白,进一步探讨HBV侵入肝细胞的机制。方法以HBV ayw亚型全长质粒PCP10为模板,PCR扩增HBV PreS1基因,克隆到酵母表达载体pSos中,构建诱饵质粒pSos-PreS1,将其转化cdc25酵母感受态细胞,提取酵母蛋白质进行Western blot分析,证实其在酵母细胞中的表达;将pSos-PreS1分别与pMyr Lamin C、pMyr SB共转化酵母,证实诱饵蛋白无自激活作用。将pSos-PreS1与人肝cDNA文库共转化cdc25酵母感受态细胞,通过营养及温度选择性培养筛选阳性菌落,扩增阳性菌落目的基因并测序。结果成功构建了HBV PreS1基因酵母双杂交诱饵载体pSos-PreS1,筛选得到5个准阳性克隆,序列分析表明其分别与人类钾通道调制因子1(KCMF1)、细胞色素C、VitD结合蛋白、人源去唾液酸糖蛋白受体(ASGPR)和人白蛋白具有高度同源性。结论利用SRS筛选出5个可能与HBV PreS1蛋白相互作用的蛋白,为进一步了解HBV侵入肝细胞的机制奠定了基础。     

钢管砼拱桥模型构建及自振特性对比分析

以云南省海口大桥为研究对象,基于ANSYS软件分别考虑墩底固结和土-桩-结构相互作用,建立了该桥的两种三维有限元模型,以此为基础进行了两种模型的自振特性对比分析;并系统地介绍了两种中承式钢管砼系杆拱桥简化模型的构建方法,可为相关研究提供参考.     

小直径钢管排桩边坡加固计算理论研究

通过分析小直径钢管排桩的受力特点,提出了一种考虑桩土相互作用空间效应的平面刚架分析模型。将桩间岩土体以水平弹簧模拟,基于Winkler弹性地基梁理论,推导了钢管排桩的内力位移计算公式。以一滑坡治理现场堆载试验为例,对提出的计算方法与现场测试数据进行分析对比,二者吻合良好,本计算方法的可靠性得到了验证。同时在此基础上,也进行了相关主要影响因子的分析,得出桩顶位移与桩长呈现函数关系。     

竖向荷载作用下预制管桩桩土共同作用的数值分析

以徐州市沛屯地区预制管桩的应用实例为基础,以FLAC3D软件为分析手段,建立了管桩桩土数值模型,通过该数值模型对管桩在竖向荷载作用下桩土共同作用机理,包括荷载传递规律、受力特性和破坏模式进行了分析,为进一步研究管桩承载机理提供了理论基础.     

冠状动脉结扎前后容量负荷对狗两心室功能的影响

本实验研究了狗左冠状动脉前降支(LAD)结扎前后,以动脉放血和静脉输液改变容量负荷(VL),对左、右心室功能的影响。结果表明,LAD 结扎前后,两心室舒缩性能和泵功能对 VL 改变的反应均有明显差别。提示,心肌梗塞后,降低 VL可改善左心室功能,应防止 VL 过重对心功能的不利影响。     

城市交通与土地利用互动机理研究

城市交通与土地利用之间存在着复杂的互动关系,研究二者的互动机理,对从根本上解决城市交通问题以及实现城市可持续发展具有重要的理论与现实意义。首先分析城市交通与土地利用的关系,指出二者之间存在相互依存、相互促进的互动关系;然后建立数学模型描述二者互动的方向及程度,从模型稳定性分析可以看出,当满足一定条件时,二者能够达到一种“互补共生”的稳定平衡状态;在此基础上,结合实例对模型进行仿真,验证了模型的稳定性条件;最后,基于二者的互动机理提出一些城市规划对策。     

A study on dynamic response of cable-seabed interaction

A numerical method is developed to investigate the dynamic response of cable-seabed interaction in this paper. The motion of cable is described by the Lumped Parameter Method, while the seabed, unlike the prevailing simplified model of elastic foundation, is modeled as an irregular continuous rigid surface with rebound and friction existing, and the forces exerted by the seabed consist of normal counterforce and isotropic tangential Coulomb friction resistance. To describe the detailed dynamic response, two coefficients are introduced by analogy with the theory of rigid body collision with friction. The cable-seabed kinematic and dynamic contact conditions are formulated subsequently, and are used to incorporate the seabed effect into the cable dynamics to produce a set of ordinary differential governing equations. In this paper, we employ 4th order Runge-Kutta method to solve these equations. Several simulation cases are presented to illustrate the seabed effect. The results show that friction and impact have a prominent influence on the statics and dynamics of the cable.     

Seasonal variability in sea surface oceanographic conditions in the Aegean Sea (Eastern Mediterranean): an overview

Seasonal variability and the spatial distribution of sea surface temperatures (SST) and salinities (SSS) are reviewed, in relation to the prevailing climatological conditions, heat fluxes, water budget and general water circulation patterns. Within this context, consideration is given to: sea surface temperatures; air temperatures; precipitation; evaporation; wind speeds and directions; freshwater (mainly riverine) discharges throughout the Aegean; and the exchange of water masses with the Black Sea and eastern Mediterranean Sea. The investigation of satellite images, covering a 6-yr period (1988–1994), has enabled a synthesis of the monthly sea surface thermal distribution to be established.The climate of the Aegean Sea is characterised by annual air temperatures of 16–19.5°C, precipitation of about 500 mm yr⁻¹ and evaporation of some 4 mm d⁻¹. The Aegean has a negative heat budget (approximately −25 W m⁻²) and positive water balance (+ 1.0 m yr⁻¹), when inflow from the Black Sea is considered. During the summer, the (northerly) Etesians are the dominant winds over the Sea.Mean monthly sea surface temperatures (SST) vary from 8°C in the north during winter, up to 26°C in the south during summer. SST depends mainly upon air temperature; there is a month's delay between the former and latter maxima. The sea surface salinity (SSS) varies also spatially and seasonally, ranging from less than 31 psu, in the north, to more than 39 psu, in the southeast; lower values (< 25 psu) occur adjacent to the river mouths. SSSs present their maximum differences during summer, whilst during winter and autumn the distribution of SSS is more uniform. The overall spatial SST and SSS distribution pattern is controlled by: distribution of the (colder) Black Sea Waters; advection of the (warmer) Levantine Waters, from the southeastern part of the Aegean; upwelling and downwelling; and, to a lesser extent, but locally important, freshwater riverine inflows.     

单桨船模自航试验的相似准则与模拟方法

本文提出一种新的船模自航模型化方法以“消除”船－桨干扰总理２的惊工影响，首先讨论船－桨组合体绕流（平均流动）应满足的相似准则，其中引进了两个新的准则以联系船 尾流的特征；尾流宽度与速度亏损，讨论了满足这些准则的模拟条件和方法，给出满足相似条件的船模－实船性能关系式，最后讨论了新模拟方法在船旋螺旋桨空的与噪声模型试验中的应用。