重组人单链白细胞介素12融合基因(rhscIL-12)的构建和真核表达

克隆人 IL - 1 2 ( h IL - 1 2 ) p40和 p35亚单位 c DNA,构建人单链 IL- 1 2 ( rhsc IL- 1 2 )融合基因 ,并在哺乳动物细胞中进行表达。方法　从经 PDBu刺激的 EBV转化的人 B淋巴母细胞株 NC37中提取 m RNA,经 RT- PCR分别获得 h IL- 1 2 p40和 p35亚单位编码序列的 c DNA,运用重组 PCR技术将两段基因通过一疏水性多肽接头 ( Gly4 Ser) 3 DNA序列进行体外基因重组 ,构建 rhsc IL- 1 2融合基因 ,将其插入 pc DNA3.1 ( + )真核表达载体 ,经脂质体转染 COS7细胞进行表达 ,Western blot进行分析。结果　所克隆的 h IL- 1 2 p40、p35c DNA序列和构建的 rhsc IL - 1 2融合基因 DNA序列均经测序得以证实 ,融合基因可在 COS7中表达其产物 rhsc IL- 1 2融合蛋白 ,其分子量为 70 KD,可与鼠抗人 IL - 1 2 p40 /p70单克隆抗体特异性结合。结论　本研究结果为进一步探讨 rhsc IL- 1 2融合蛋白的生物学活性和特性奠定了基础 ,也为 rhsc IL- 1 2融合基因在原核细胞中的表达提供了可能性     

A rnold-Chiari畸形的诊断与外科治疗

探讨 Arnold- Chiari畸形的 MRI诊断及外科治疗效果。方法　回顾性分析了 1 2例 Arnold- Chiari畸形患者的临床表现、MRI诊断及手术方式和疗效。结果　术前 1 2例患者均经 MRI诊断。 4例行单纯后颅窝及颈 1～ 2椎板减压术 ,8例行后颅窝及颈椎板减压术加同期脊髓空洞—蛛网膜下腔分流术 ,手术有效率为 83%。结论　 MRI是 Arnold- Chiari畸形并脊髓空洞的最佳诊断手段 ;Arnold- Chiari畸形合并较大脊髓空洞时应同期行脊髓空洞—蛛网膜下腔分流术     

THE INVESTIGATION OF DIFFERENT POPULATION＇S TRANSFUSION-TRANSMITTED VIRUS-DNA IN SHAANXI PROVINCE

Objective To investigate transfusion transmitted virus (TTV) infection among population of different groups in Shaanxi Province. Methods A nested polymerase chain reaction (PCR) with primers from ORF1 of TTV genome was established to detect TTV-DNA in serum of the patients. Results TTV-DAN was detected in the sera of 3 of 50 cases of general population(6％), 2 of 30 cases of vocational blood donors(6.7%),21 of 97 cases with Type B hepatitis (21. 6％), 9 of 35 cases of Type C hepatitis (25. 7％),and 23 of 40 cases with non-A～ non-G hepatitis (57.5 ％ ). Conclusion There is TTV infection among general population in Shaanxi Province. TTV may be an impor- tant agent to cause non-A～non-G hepatitis. And the patients with HBV or HCV can have overlapping TTV infection.     

EXPRESSIONS OF P53, PROLIFERATING CELL NUCLEAR ANITIGEN, BCL-2 PROTEIN AND THEIR SIGNIFICANCE IN SALIVARY ADENOID CYSTIC CARCINOMA

Studiesonthecarcinomasshowedthatthespeedofcarcinomadevelopmentisdependentonthebalanceofcellproliferatingandapoptosis.Soitisveryimportanttostudythemechanismbywhichoncogenescontrolproliferationandapoptosisoftu-morcell.Moststudiesonsalivaryglandtumorshavelaidemphsisontheproliferatingfunctionofonco-genesathomeandabroad,andignoranteditsfunc-tiononapoptosis,especially,thestudieswerefew-.erincombiningtwokindsoffunction.Helen's[13studyonACCindicatedthatmutationofP,3doesn'tplayimportantroleincarcinog…     

RAPID MOLECULAR DETECTION OF METHICILLIN-RESISTANT S. AUREUS IN THE INTENSIVE CARE UNIT

Staphylococcus aureus is found in the nose andon the skin of a variety of healthy individuals,andis an opportunistpathogen in patients with loweredhost resistance.The isoxazolylpenicillin (e.g.me-thicillin,cloxacillin,flucloxacillin) have been themainstay of treatment for infection over2 5years.But emergence of antibiotic resistance(MRSA) is aproblem in hospitals due to transmission of epi-demic MRSA strains with antibiotic resistance.Ac-quisition of MRSA continues to be of major con-cern…     

CLONING AND SEQUENCING OF MATURE FRAGMENT OF HUMAN BMP4 GENE

Bone morphogenetic protein- 4(BMP- 4) is alow molecular weight glycoprotein ,classified as amorphogen.It is capable of inducing the formationof new cartilage and bone.This osteoinductiveability has led to the use of bone morphogeneticproteins as therapeutic agents for creation of newbone useful in treatment of skeletal injuries anddiseases,and in oral and maxillofacial applica-tions.Although many researchers have got the na-tive BMP from animal s demineralized bone by bio-chemical method,the…     

褪黑素对链脲佐菌素所致胰岛β细胞损伤的保护作用研究

用链脲佐菌素 (STZ)作用于离体胰岛β细胞 2 4 h,一氧化氮 (NO)、脂质过氧化产物丙二醛(MDA)生成增多 ,胰岛细胞膜流动性下降 ,微粘度增高 ,同时胰岛素分泌功能降低 ,细胞存活率下降。预先给高浓度褪黑素 (10 μmol/ L)处理 2 4 h者 ,可减少 NO、MDA的释放 ,提高超氧化物歧化酶的水平 ,使胰岛细胞的氧化损伤减轻 ,膜微粘度降低 ,膜流动性增加 ,细胞存活率显著提高。但低浓度褪黑素 (10 nmol/ L)无此作用。提示高浓度褪黑素对 STZ所致的胰岛 β细胞损伤有一定的保护作用。     

基于体积力法的半悬挂舵和全悬挂舵回转性对比

针对半悬挂舵和全悬挂舵回转性能差异问题,建立用于模拟螺旋桨推力的体积力模型,评估两种不同形式舵的回转性。对标模KCS船型回转运动进行计算流体动力学（Computational Fluid Dynamics,CFD）模拟,船后螺旋桨作用使用体积力模型替代。开展半悬挂舵回转性CFD模拟,并与试验结果对比验证。设计全悬挂舵并模拟其回转性,与半悬挂舵回转性进行比较。结果显示,半悬挂舵除战术直径与试验结果相差9.48%外,其他回转特性参数与试验结果相差均在3%以内,说明基于体积力法模拟回转性具有较高的可信度。全悬挂舵的回转特性参数均优于半悬挂舵,说明全悬挂舵的回转性能更佳。     

关角隧道设计方案比选

详细介绍了青藏线西格二线关角隧道的线路方案比选、施工方案的比选，主要进行了关角隧道钻爆法与TBM法施工的方案分析，以为国内其它长大隧道设计提供参考。     

地铁屏蔽门渗漏风量的计算分析及应用

以广州地铁某典型地下岛式车站为例,采用 通风网络计算法,分析计算列车停站期间通过车站屏 蔽门( PSD) 的渗漏风量; 与实际测试结果进行分析对 比,验证计算结果的准确性。实践表明,通风网络计算 法可以用来计算屏蔽门的渗漏风量,能克服实测中测 试设备布置的不便及数值模拟中建模时间长、计算复 杂的弊端,是一种快捷、准确且满足工程精度要求的计 算方法,对地铁车站空调通风系统的设计及运营有重 要的指导意义。