谨记饭后的“八个不急”

一、不急于吸烟 饭后吸烟的危害比平时大10倍。这是由于进食后的消化道血液循环量增多，致使烟中有害成分大量吸收而损害肝、脑及心脏血管。专家分析：从医学角度来分析一下饭后吸烟的害处．是很明显的事实，显而易见。当人进食以后，消化系统立刻全面运动起来，进行消化和吸收等各种生理活动。     

PRELIMINARY STUDY OF A NOVEL HUMAN PAPILLOMAVIRUS TYPE 16L1／E6-E7 CHIMERIC RECOMBINANT DNA VACCINE

Objective Preparations of HPV16 L1/E6 and L1/E7 prophylactic and therapeutic DNA vaccines. Methods The nucleotides within HPV16 E6 and E7 genes, which are responsible for viral transforming activity, were mutated by mage primer site-directed mutagenesis method. The correctly mutated E6 and E7 fragments were separately cloned into an eukaryotic expression vector pVAX1, together with HPV16 L1 gene, generating chimeric recombinants plasmids 1MpVAX1-L1E6, 2MpVAX1-L1E6, 1MpVAX1-L1E7, 2MpVAX1-L1E7 and 3MpVAX1-L1E7. CHO cells were transiently transfected with the individual DNA vaccines by calcium phosphate method. Target protein expressions in the extracts of the transfected cell lines were measured by ELISA and immunohistochemistry, with HPV16 L1 and E6 specific monoclonal antibodies. Results ELISA assays showed the P/N ratios in the cell extracts transfected with L1E6 and L1E7 plasmids were more than 2.1. Immunohistochemistry revealed brownish precipitant signal in cytoplasm and nuclei of the transfected cells. Conclusion Successful constructions of prophylactic and therapeutic DNA vaccine plasmids lay solid foundation for future animal experiment and clinical trial.     

重症患者TAT/PIC水平与机械通气使用率间的关系

目的 探讨成年重症患者凝血酶-抗凝血酶复合物(thrombin-antithrombin complex, TAT)/纤溶酶-α2纤溶酶抑制物复合物(α2-plasmin inhibitor-plasmin complex, PIC)(TAT/PIC)水平与机械通气(mechanical ventilation, MV)使用率间的关系。方法 采用横断面研究,纳入单次入重症医学科且入科时长≥1 d且有血栓前四项检测记录的成年患者。在医院信息系统及实验室信息系统中回顾性收集患者年龄、性别、首次血栓前四项检测结果、弥散性血管内凝血(disseminated intravascular coagulation, DIC)评分、治疗方式及所有诊断信息。采用Logistic回归模型探讨TAT/PIC与MV使用率间的关系;采用交互作用分析、亚组分析探讨在不同年龄、性别、感染/未感染、患/未患DIC患者中TAT/PIC水平与MV使用间的关系是否存在统计学差异。结果 本研究共纳入1 176名患者,所有患者首次TAT/PIC的中位数为15.84(8.13-33.11)。多因素Logistic回归分析结果显...     

船舶舱室大气环境感染控制设计研究

对船舶舱室大气环境感染控制设计进行了研究.针对船舶的流动性、晃动性和空间局限性,对进、排风口选取和压差扰动因素规避及系统设备运行等,提出了控制设计方法.探讨舱室内新风量的选取和隔离病房内气流的组织形式.利用软件FloEFD对3种气流组织形式进行了比较,从降低交叉感染几率,保护医护工作者的角度,分析降低污染物浓度的清除效...