HPLC法测定健宝胶囊中淫羊藿苷含量

目的　建立健宝胶囊中淫羊藿苷的含量测定方法。方法　采用高效液相色谱法 ,色谱柱为C18(15 0mm×4.6mm) ,流动相为乙腈 0 .0 75mol·L-1磷酸溶液 (2 5∶75 ) ,流速 1.0mL·min-1,检测波长为 2 70nm。结果　淫羊藿苷进样量在 0 .0 6～ 0 .30 μm范围内 ,线性关系良好 (r=0 .9991) ,方法回收率为 98.5 0 %(n =5 ,RSD =1.2 0 %)。结论　本方法准确、简便、快速 ,适合于该药的质量分析检验。     

Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin （HSA） was investigated using indirect chiral high performance liquid chromatography （HPLC） and ultrafiltration techniques. S-（-）-1-（1-naphthyl）- ethylamine （S-NEA） was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of S-enantiomer was stronger than R-enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.     

Comparative pharmacokinetics of chlorogenic acid after oral administration in rats

The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlo...     

多敏灵片中咖啡因和盐酸多塞平含量的测定

目的建立高效液相色谱法,测定多敏灵片中咖啡因、盐酸多塞平的含量。方法采用高效液相色谱法,以Kromasil C18(250 mm×4.6 mm,5μm)为分析柱,流动相为甲醇-水-三乙胺(75∶25∶1),流速1 mL/min,检测波长295 nm,柱温为室温。结果咖啡因、盐酸多塞平与其他组分分离良好,且不受其他成分的干扰;咖啡因回归方程为:Y=196 604X+218 976(r=0.999 8,n=5),咖啡因在0.08~0.24μg的范围内线性关系良好;盐酸多塞平回归方程为:Y=60 158X+58 230(r=0.999 9,n=5),盐酸多塞平在0.04~0.12μg的范围内线性关系良好。方法的平均回收率分别为99.06%、96.67%,精密度(RSD)值分别为1.04%、1.13%。结论本方法操作简便、快速、准确、灵敏,适用于多敏灵片的含量测定。     

HPLC法测定复方新诺明的含量

方法：采用高效液相色谱法测定复方新诺明片中磺胺甲嗯唑和甲氧苄啶含量。用C18。柱（5μm，0．46cm×25cm），用冰醋酸调节pH值至5．90±0．10，以水-乙腈-三乙胺（799：200：1）为流动相，流速为1．2ml／min，检测波长为240nm。结果：制剂中磺胺甲嗯唑和甲氧苄啶峰面积与含量的相关系数分别为0．9993和0．9995，回收率在99．1％和98．9％。结论：该方法分离效果好，适于制剂的质量控制与检测。     

平波电抗器高温过热故障的检测与诊断

通过检测分析变压器油中特征气体组分含量，考察油中特征气体组分的绝对产气速率，判断SS4改型电力机车变压器内部存在高温过热故障，经解体检查为平波电抗器牵头铜排烧断。     

Stability indicating high performance thin-layer chromatographic method for simultaneous estimation of pantoprazole sodium and itopride hydrochloride in combined dosage form

A specific, precise and stability indicating high-performance thin-layer chromatographic method for simultaneous estimation of pantoprazole sodium and itopride hydrochloride in pharmaceutical formulati...     

A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection

A rapid method has been developed based on the sample preparation procedure named as QuEChERS （Quick, Easy, Cheap, Effective, Rugged and Safe）, combined with reversed-phase high performance liquid chromatography with fluorescence detector and C18 column after precolumn derivatization using o-phthalaldehyde and 2-mercaptoethanol to determine dopamine in porcine muscle. Methanol and deionized water （0.1% acetic acid, v/v） with a ratio of 60：40 was used as mobile phase. The flow rate was 0.8 mL/min and dopamine was eluted within 15 min. The linearity range was 0.003-8 μg/mL with r=0.9992. The detection limit for dopamine was 4 μg/kg and the quantification limit was 9 μg/kg. Recovery studies were carried out at 0.1, 0.5 and 1.0 mg/kg fortification levels and the average recoveries obtained ranged from 90.4% to 98.2% with relative standard deviations between 3.5% and 8.1%. The method was found to be suitable for detection of dopamine in animal product tissues at the maximum residue level.     

RP-HPLC method for the quantitation of β-Sitosterol in Elaeagnus Gon yanthes Benth

Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth. Methods The separation was performed on a luna C8 (2) (150 mm×4.6 mm, 5μm) column with the mobile phase of methanol-water (88∶12, v/v) at a flow rate of 1.0 mL/min, the detection wavelength was set at 210 nm, and the temperature of the column was maintained at 35 ℃. Results The calibration curve of β-Sitosterol was linear over the concentration range of 0.075-0.375 mg/mL (r=0.9999) and the average recovery of β-Sitosterol was 96.30% with RSD of 3.60%(n=3). Conclusion The method is simple, rapid, and accurate, and can be used for the quality control of Elaeagnus Gonyanthes Benth.